SSGCID: Target Page

AcbaC.00113.a

3-oxoacyl-[acyl-carrier-protein] synthase 1

CENTER ID:	AcbaC.00113.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00113.a.B1.GE38586	full length		1	409

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.3016
GenBank:	AKA32778.1
PGAT:	acinetobacter:ABUW_3065
RefSeq:	WP_000832710.1
UniProt:	V5VFT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRVVITGMG INSCIGNSLE EVTHSLKNGI SGTRFNPTYA ELNFKSHVSA AAEQDFDNID RKLKRFMGVC AMYAYNSAVA AVEHAGLKAE DLADNPRYGI AGGSGGGSTA SVVEMTELLE TKGARKVGPF FVPRNMTNTI TANVGVAFKL QGIAHSIASA CATSADAIGY AYNLIALGKQ DLMLAGGGEE DHWSQSLLFD AMGALCSKYN DTPETASRPY SKDRDGFVIA GGGGFVVLES LEHAQARGAN ILAEVVAYAA NSDGADMVAP SGEGATRCIL MALEEAKQHG VDKIDYVNTH GTSTPAGDVT ELKAMERAFG EGKVPPLSST KSMTGHSLGA AGVHEAIYSV LMLQNDFIAP NINVTELDEG TEGFDIVLEK RDTKLNTVMS NSFGFGGVNA CLIFKKWDA

NT Sequence

ATGAAACGTG TTGTAATCAC TGGTATGGGT ATTAACTCAT GTATCGGTAA CTCTTTAGAA GAAGTTACTC ATTCACTTAA AAATGGAATC TCCGGGACAC GTTTTAACCC AACTTATGCT GAACTCAATT TTAAAAGTCA TGTCAGCGCT GCGGCAGAAC AAGATTTTGA CAATATTGAT CGTAAATTAA AACGGTTCAT GGGCGTATGT GCAATGTACG CTTATAACTC TGCTGTTGCA GCCGTTGAAC ATGCTGGCTT AAAAGCAGAA GATTTAGCAG ATAATCCACG TTATGGTATT GCCGGTGGTT CAGGCGGTGG TTCAACTGCT TCTGTTGTAG AAATGACTGA ACTTTTAGAA ACTAAAGGTG CTCGTAAAGT TGGTCCTTTC TTTGTTCCAC GTAATATGAC AAATACAATC ACTGCAAACG TGGGTGTAGC ATTTAAACTT CAAGGTATTG CTCACTCAAT TGCAAGTGCT TGTGCAACTT CTGCAGACGC AATTGGTTAT GCATATAACC TTATCGCTCT AGGCAAACAA GATCTTATGC TTGCTGGTGG TGGTGAAGAA GATCACTGGT CTCAAAGCCT ATTGTTTGAT GCAATGGGTG CTTTGTGTTC TAAATACAAC GACACACCTG AAACAGCGTC TCGTCCATAT TCTAAAGACC GTGACGGTTT CGTTATTGCT GGCGGTGGCG GTTTCGTTGT TCTTGAATCT CTTGAACATG CTCAAGCACG TGGTGCAAAT ATTCTTGCTG AAGTTGTTGC TTATGCTGCA AACAGTGACG GCGCTGACAT GGTAGCTCCA AGTGGTGAAG GTGCAACTCG CTGTATCTTG ATGGCTCTTG AAGAAGCGAA ACAACACGGC GTAGATAAAA TTGACTATGT AAATACGCAT GGTACTTCTA CACCAGCAGG TGACGTGACT GAGCTTAAAG CAATGGAACG TGCTTTCGGT GAAGGTAAAG TTCCACCTCT TAGTTCAACT AAATCTATGA CTGGTCACAG CCTAGGTGCT GCTGGTGTTC ATGAAGCAAT TTACTCTGTA TTAATGCTTC AAAATGACTT TATCGCGCCA AACATCAACG TGACTGAGCT TGATGAAGGT ACTGAAGGCT TTGATATTGT GCTTGAAAAA CGTGATACAA AATTGAATAC TGTAATGAGT AACAGTTTTG GCTTTGGCGG TGTTAACGCT TGCCTAATTT TCAAGAAGTG GGATGCATAA

Details for AcbaC.00113.a.B1.GE38586

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	44kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMK RVVITGMGIN SCIGNSLEEV THSLKNGISG TRFNPTYAEL NFKSHVSAAA EQDFDNIDRK LKRFMGVCAM YAYNSAVAAV EHAGLKAEDL ADNPRYGIAG GSGGGSTASV VEMTELLETK GARKVGPFFV PRNMTNTITA NVGVAFKLQG IAHSIASACA TSADAIGYAY NLIALGKQDL MLAGGGEEDH WSQSLLFDAM GALCSKYNDT PETASRPYSK DRDGFVIAGG GGFVVLESLE HAQARGANIL AEVVAYAANS DGADMVAPSG EGATRCILMA LEEAKQHGVD KIDYVNTHGT STPAGDVTEL KAMERAFGEG KVPPLSSTKS MTGHSLGAAG VHEAIYSVLM LQNDFIAPNI NVTELDEGTE GFDIVLEKRD TKLNTVMSNS FGFGGVNAC

Validated NT Sequence

taggcaagcg ttaacaccgc caaagccaaa actgttactc attacagtat tcaattttgt atcacgtttt tcaagcacaa tatcaaagcc ttcagtacct tcatcaagct cagtcacgtt gatgtttggc gcgataaagt cattttgaag cattaataca gagtaaattg cttcatgaac accagcagca cctaggctgt gaccagtcat agatttagtt gaactaagag gtggaacttt accttcaccg aaagcacgtt ccattgcttt aagctcagtc acgtcacctg ctggtgtaga agtaccatgc gtatttacat agtcaatttt atctacgccg tgttgtttcg cttcttcaag agccatcaag atacagcgag ttgcaccttc accacttgga gctaccatgt cagcgccgtc actgtttgca gcataagcaa caacttcagc aagaatattt gcaccacgtg cttgagcatg ttcaagagat tcaagaacaa cgaaaccgcc accgccagca ataacgaaac cgtcacggtc tttagaatat ggacgagacg ctgtttcagg tgtgtcgttg tatttagaac acaaagcacc cattgcatca aacaataggc tttgagacca gtgatcttct tcaccaccac cagcaagcat aagatcttgt ttgcctagag cgataaggtt atatgcataa ccaattgcgt ctgcagaagt tgcacaagca cttgcaattg agtgagcaat accttgaagt ttaaatgcta cacccacgtt tgcagtgatt gtatttgtca tattacgtgg aacaaagaaa ggaccaactt tacgagcacc tttagtttct aaaagttcag tcatttctac aacagaagca gttgaaccac cgcctgaacc accggcaata ccataacgtg gattatctgc taaatcttct gcttttaagc cagcatgttc aacggctgca acagcagagt tataagcgta cattgcacat acgcccatga accgttttaa tttacgatca atattgtcaa aatcttgttc tgccgcagcg ctgacatgac ttttaaaatt gagttcagca taagttgggt taaaacgtgt cccggagatt ccatttttaa gtgaatgagt aacttcttct aaagagttac cgatacatga gttaataccc ataccagtga ttacaacacg tttcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac gtgttgtaat cactggtatg ggtattaact catgtatcgg taactcttta gaagaagtta ctcattcact taaaaatgga atctccggga cacgttttaa cccaacttat gctgaactca attttaaaag tcatgtcagc gctgcggcag aacaagattt tgacaatatt gatcgtaaat taaaacggtt catgggcgta tgtgcaatgt acgcttataa ctctgctgtt gcagccgttg aacatgctgg cttaaaagca gaagatttag cagataatcc acgttatggt attgccggtg gttcaggcgg tggttcaact gcttctgttg tagaaatgac tgaactttta gaaactaaag gtgctcgtaa agttggtcct ttctttgttc cacgtaatat gacaaataca atcactgcaa acgtgggtgt agcatttaaa cttcaaggta ttgctcactc aattgcaagt gcttgtgcaa cttctgcaga cgcaattggt tatgcatata accttatcgc tctaggcaaa caagatctta tgcttgctgg tggtggtgaa gaagatcact ggtctcaaag cctattgttt gatgcaatgg gtgctttgtg ttctaaatac aacgacacac ctgaaacagc gtctcgtcca tattctaaag accgtgacgg tttcgttatt gctggcggtg gcggtttcgt tgttcttgaa tctcttgaac atgctcaagc acgtggtgca aatattcttg ctgaagttgt tgcttatgct gcaaacagtg acggcgctga catggtagct ccaagtggtg aaggtgcaac tcgctgtatc ttgatggctc ttgaagaagc gaaacaacac ggcgtagata aaattgacta tgtaaatacg catggtactt ctacaccagc aggtgacgtg actgagctta aagcaatgga acgtgctttc ggtgaaggta aagttccacc tcttagttca actaaatcta tgactggtca cagcctaggt gctgctggtg ttcatgaagc aatttactct gtattaatgc ttcaaaatga ctttatcgcg ccaaacatca acgtgactga gcttgatgaa ggtactgaag gctttgatat tgtgcttgaa aaacgtgata caaaattgaa tactgtaatg agtaacagtt ttggctttgg cggtgttaac gcttgcctaa ttttcaagaa gtgggatgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat