AcbaC.00111.a

glutamine synthetase, type I

CENTER ID: AcbaC.00111.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00111.a.B1.GE38585 full length 1 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00111.a.B1.PW37680 full length 1 471

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.1171
GenBank: AKA30958.1
PGAT: acinetobacter:ABUW_1207
UniProt: A0A090B781

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMANKVLQL IQESGAKWVD FRFTDTKGKE QHVTYPADSI DEDTFEDGKM FDGSSIAGWK GIEASDMILR PDAETGFIDP FFAEPTVVVT CDVIEPSTGQ GYERDPRSIA RRAEEYLKST GIGDTAFFGP EPEFFVFDEV KWDIDMSGAR HTLIAEEAAW STGKDYESGN SGHRPRVKGG YFPVPPVDSA QDMRAEMCAK IEDIMGPGRV EVHHHEVASC QLEIGVSFNT LVRKADEVQQ FKYAVWNVAH QYAKTATFMP KPMVGDNGSG MHVHMSISKD GKNLFAGDEY AGLSEMALYF IGGIIKHARA LNAITNPSTN SYKRLVPHFE APIMLAYSAR NRSASIRIPY VSNPKGKRIE ARFPDPMMNP YLGFAALLMA GIDGIQNKIH PGEAADKNLY DLPPEEEAKI PTVAHSLDMA LEALQADHEF LLKGGVFTKE MLDAYIELKT EDVRRLNTTT HPVEFDMYYS L
NT Sequence
ATGAGCATGG CGAACAAGGT CCTTCAACTC ATACAAGAAA GTGGCGCAAA ATGGGTCGAT TTTCGCTTTA CTGATACTAA GGGTAAAGAA CAGCACGTAA CTTACCCAGC TGATTCTATT GATGAAGATA CTTTTGAAGA CGGTAAAATG TTCGATGGTT CTTCAATCGC TGGTTGGAAA GGTATTGAAG CATCTGACAT GATTTTACGT CCGGATGCAG AAACTGGTTT CATCGACCCG TTCTTTGCTG AGCCAACAGT AGTTGTGACT TGTGACGTTA TTGAACCTTC AACTGGTCAA GGTTATGAAC GTGACCCACG TTCGATTGCT CGCCGTGCAG AAGAATACTT AAAATCTACA GGTATCGGTG ATACTGCATT CTTTGGTCCA GAGCCAGAAT TCTTTGTATT TGACGAAGTG AAATGGGACA TCGATATGTC TGGCGCTCGT CATACATTAA TCGCTGAAGA GGCTGCTTGG TCTACTGGTA AAGATTACGA GTCTGGTAAC TCAGGTCACC GTCCACGTGT TAAAGGTGGT TACTTCCCAG TTCCTCCAGT TGACTCTGCA CAAGATATGC GTGCAGAAAT GTGTGCAAAA ATTGAAGACA TTATGGGTCC AGGTCGTGTA GAAGTACACC ACCACGAAGT TGCTTCTTGC CAATTAGAAA TTGGTGTGAG CTTCAATACG CTTGTACGTA AAGCTGACGA AGTTCAACAG TTCAAATATG CTGTTTGGAA CGTTGCACAT CAATATGCAA AAACAGCTAC GTTTATGCCT AAACCAATGG TAGGTGATAA CGGTTCTGGT ATGCACGTTC ACATGTCTAT CTCTAAAGAT GGTAAGAACT TGTTTGCTGG TGATGAATAT GCAGGCCTTT CAGAAATGGC GTTGTACTTC ATCGGTGGTA TCATCAAGCA CGCTCGTGCA TTGAATGCAA TCACAAACCC ATCTACAAAC TCATACAAGC GTTTGGTTCC TCACTTCGAA GCACCAATTA TGCTTGCTTA CTCAGCGCGT AACCGTTCTG CGTCTATCCG TATTCCTTAC GTTTCTAACC CTAAAGGCAA GCGTATCGAA GCTCGTTTCC CAGACCCAAT GATGAACCCG TACTTAGGTT TCGCTGCGTT ATTAATGGCT GGTATCGACG GTATCCAAAA CAAGATCCAT CCGGGCGAAG CTGCTGACAA GAACTTGTAC GATCTTCCTC CAGAAGAAGA AGCAAAAATC CCAACAGTTG CTCATAGCCT AGATATGGCT CTTGAAGCAC TTCAAGCAGA TCACGAATTC TTGTTAAAAG GTGGCGTGTT CACTAAAGAA ATGCTTGATG CATACATCGA ACTTAAAACT GAAGATGTAC GTCGTCTTAA CACGACTACT CACCCAGTTG AATTCGATAT GTACTACAGC CTGTAA
Details for AcbaC.00111.a.B1.GE38585
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS MANKVLQLIQ ESGAKWVDFR FTDTKGKEQH VTYPADSIDE DTFEDGKMFD GSSIAGWKGI EASDMILRPD AETGFIDPFF AEPTVVVTCD VIEPSTGQGY ERDPRSIARR AEEYLKSTGI GDTAFFGPEP EFFVFDEVKW DIDMSGARHT LIAEEAAWST GKDYESGNSG HRPRVKGGYF PVPPVDSAQD MRAEMCAKIE DIMGPGRVEV HHHEVASCQL EIGVSFNTLV RKADEVQQFK YAVWNVAHQY AKTATFMPKP MVGDNGSGMH VHMSISKDGK NLFAGDEYAG LSEMALYFIG GIIKHARALN AITNPSTNSY KRLVPHFEAP IMLAYSARNR SASIRIPYVS NPKGKRIEAR FPDPMMNPYL GFAALLMAGI DGIQNKIHPG EAADKNLYDL PPEEEAKIPT VAHSLDMALE ALQADHEFLL KGGVFTKEML DAYIELKTED VRRLNTT
Validated NT Sequence
agtagtcgtg ttaagacgac gtacatcttc agttttaagt tcgatgtatg catcaagcat ttctttagtg aacacgccac cttttaacaa gaattcgtga tctgcttgaa gtgcttcaag agccatatct aggctatgag caactgttgg gatttttgct tcttcttctg gaggaagatc gtacaagttc ttgtcagcag cttcgcccgg atggatcttg ttttggatac cgtcgatacc agccattaat aacgcagcga aacctaagta cgggttcatc attgggtctg ggaaacgagc ttcgatacgc ttgcctttag ggttagaaac gtaaggaata cggatagacg cagaacggtt acgcgctgag taagcaagca taattggtgc ttcgaagtga ggaaccaaac gcttgtatga gtttgtagat gggtttgtga ttgcattcaa tgcacgagcg tgcttgatga taccaccgat gaagtacaac gccatttctg aaaggcctgc atattcatca ccagcaaaca agttcttacc atctttagag atagacatgt gaacgtgcat accagaaccg ttatcaccta ccattggttt aggcataaac gtagctgttt ttgcatattg atgtgcaacg ttccaaacag catatttgaa ctgttgaact tcgtcagctt tacgtacaag cgtattgaag ctcacaccaa tttctaattg gcaagaagca acttcgtggt ggtgtacttc tacacgacct ggacccataa tgtcttcaat ttttgcacac atttctgcac gcatatcttg tgcagagtca actggaggaa ctgggaagta accaccttta acacgtggac ggtgacctga gttaccagac tcgtaatctt taccagtaga ccaagcagcc tcttcagcga ttaatgtatg acgagcgcca gacatatcga tgtcccattt cacttcgtca aatacaaaga attctggctc tggaccaaag aatgcagtat caccgatacc tgtagatttt aagtattctt ctgcacggcg agcaatcgaa cgtgggtcac gttcataacc ttgaccagtt gaaggttcaa taacgtcaca agtcacaact actgttggct cagcaaagaa cgggtcgatg aaaccagttt ctgcatccgg acgtaaaatc atgtcagatg cttcaatacc tttccaacca gcgattgaag aaccatcgaa cattttaccg tcttcaaaag tatcttcatc aatagaatca gctgggtaag ttacgtgctg ttctttaccc ttagtatcag taaagcgaaa atcgacccat tttgcgccac tttcttgtat gagttgaagg accttgttcg ccatgctcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS MANKVLQLIQ ESGAKWVDFR FTDTKGKEQH VTYPADSIDE DTFEDGKMFD GSSIAGWKGI EASDMILRPD AETGFIDPFF AEPTVVVTCD VIEPSTGQGY ERDPRSIARR AEEYLKSTGI GDTAFFGPEP EFFVFDEVKW DIDMSGARHT LIAEEAAWST GKDYESGNSG HRPRVKGGYF PVPPVDSAQD MRAEMCAKIE DIMGPGRVEV HHHEVASCQL EIGVSFNTLV RKADEVQQFK YAVWNVAHQY AKTATFMPKP MVGDNGSGMH VHMSISKDGK NLFAGDEYAG LSEMALYFIG GIIKHARALN AITNPSTNSY KRLVPHFEAP IMLAYSARNR SASIRIPYVS NPKGKRIEAR FPDPMMNPYL GFAALLMAGI DGIQNKIHPG EAADKNLYDL PPEEEAKIPT VAHSLDMALE ALQADHEFLL KGGVFTKEML DAYIELKTED VRRLNTTTHP VEFDMYYSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca tggcgaacaa ggtccttcaa ctcatacaag aaagtggcgc aaaatgggtc gattttcgct ttactgatac taagggtaaa gaacagcacg taacttaccc agctgattct attgatgaag atacttttga agacggtaaa atgttcgatg gttcttcaat cgctggttgg aaaggtattg aagcatctga catgatttta cgtccggatg cagaaactgg tttcatcgac ccgttctttg ctgagccaac agtagttgtg acttgtgacg ttattgaacc ttcaactggt caaggttatg aacgtgaccc acgttcgatt gctcgccgtg cagaagaata cttaaaatct acaggtatcg gtgatactgc attctttggt ccagagccag aattctttgt atttgacgaa gtgaaatggg acatcgatat gtctggcgct cgtcatacat taatcgctga agaggctgct tggtctactg gtaaagatta cgagtctggt aactcaggtc accgtccacg tgttaaaggt ggttacttcc cagttcctcc agttgactct gcacaagata tgcgtgcaga aatgtgtgca aaaattgaag acattatggg tccaggtcgt gtagaagtac accaccacga agttgcttct tgccaattag aaattggtgt gagcttcaat acgcttgtac gtaaagctga cgaagttcaa cagttcaaat atgctgtttg gaacgttgca catcaatatg caaaaacagc tacgtttatg cctaaaccaa tggtaggtga taacggttct ggtatgcacg ttcacatgtc tatctctaaa gatggtaaga acttgtttgc tggtgatgaa tatgcaggcc tttcagaaat ggcgttgtac ttcatcggtg gtatcatcaa gcacgctcgt gcattgaatg caatcacaaa cccatctaca aactcataca agcgtttggt tcctcacttc gaagcaccaa ttatgcttgc ttactcagcg cgtaaccgtt ctgcgtctat ccgtattcct tacgtttcta accctaaagg caagcgtatc gaagctcgtt tcccagaccc aatgatgaac ccgtacttag gtttcgctgc gttattaatg gctggtatcg acggtatcca aaacaagatc catccgggcg aagctgctga caagaacttg tacgatcttc ctccagaaga agaagcaaaa atcccaacag ttgctcatag cctagatatg gctcttgaag cacttcaagc agatcacgaa ttcttgttaa aaggtggcgt gttcactaaa gaaatgcttg atgcatacat cgaacttaaa actgaagatg tacgtcgtct taacacgact actcacccag ttgaattcga tatgtactac agcctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for AcbaC.00111.a.B1.PW37680
PURIFICATION DATe: 3/24/2015
CONCENTRATION: 14mg/ml
OBSERVED MW: 64kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS MANKVLQLIQ ESGAKWVDFR FTDTKGKEQH VTYPADSIDE DTFEDGKMFD GSSIAGWKGI EASDMILRPD AETGFIDPFF AEPTVVVTCD VIEPSTGQGY ERDPRSIARR AEEYLKSTGI GDTAFFGPEP EFFVFDEVKW DIDMSGARHT LIAEEAAWST GKDYESGNSG HRPRVKGGYF PVPPVDSAQD MRAEMCAKIE DIMGPGRVEV HHHEVASCQL EIGVSFNTLV RKADEVQQFK YAVWNVAHQY AKTATFMPKP MVGDNGSGMH VHMSISKDGK NLFAGDEYAG LSEMALYFIG GIIKHARALN AITNPSTNSY KRLVPHFEAP IMLAYSARNR SASIRIPYVS NPKGKRIEAR FPDPMMNPYL GFAALLMAGI DGIQNKIHPG EAADKNLYDL PPEEEAKIPT VAHSLDMALE ALQADHEFLL KGGVFTKEML DAYIELKTED VRRLNTT
Validated NT Sequence
agtagtcgtg ttaagacgac gtacatcttc agttttaagt tcgatgtatg catcaagcat ttctttagtg aacacgccac cttttaacaa gaattcgtga tctgcttgaa gtgcttcaag agccatatct aggctatgag caactgttgg gatttttgct tcttcttctg gaggaagatc gtacaagttc ttgtcagcag cttcgcccgg atggatcttg ttttggatac cgtcgatacc agccattaat aacgcagcga aacctaagta cgggttcatc attgggtctg ggaaacgagc ttcgatacgc ttgcctttag ggttagaaac gtaaggaata cggatagacg cagaacggtt acgcgctgag taagcaagca taattggtgc ttcgaagtga ggaaccaaac gcttgtatga gtttgtagat gggtttgtga ttgcattcaa tgcacgagcg tgcttgatga taccaccgat gaagtacaac gccatttctg aaaggcctgc atattcatca ccagcaaaca agttcttacc atctttagag atagacatgt gaacgtgcat accagaaccg ttatcaccta ccattggttt aggcataaac gtagctgttt ttgcatattg atgtgcaacg ttccaaacag catatttgaa ctgttgaact tcgtcagctt tacgtacaag cgtattgaag ctcacaccaa tttctaattg gcaagaagca acttcgtggt ggtgtacttc tacacgacct ggacccataa tgtcttcaat ttttgcacac atttctgcac gcatatcttg tgcagagtca actggaggaa ctgggaagta accaccttta acacgtggac ggtgacctga gttaccagac tcgtaatctt taccagtaga ccaagcagcc tcttcagcga ttaatgtatg acgagcgcca gacatatcga tgtcccattt cacttcgtca aatacaaaga attctggctc tggaccaaag aatgcagtat caccgatacc tgtagatttt aagtattctt ctgcacggcg agcaatcgaa cgtgggtcac gttcataacc ttgaccagtt gaaggttcaa taacgtcaca agtcacaact actgttggct cagcaaagaa cgggtcgatg aaaccagttt ctgcatccgg acgtaaaatc atgtcagatg cttcaatacc tttccaacca gcgattgaag aaccatcgaa cattttaccg tcttcaaaag tatcttcatc aatagaatca gctgggtaag ttacgtgctg ttctttaccc ttagtatcag taaagcgaaa atcgacccat tttgcgccac tttcttgtat gagttgaagg accttgttcg ccatgctcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMS MANKVLQLIQ ESGAKWVDFR FTDTKGKEQH VTYPADSIDE DTFEDGKMFD GSSIAGWKGI EASDMILRPD AETGFIDPFF AEPTVVVTCD VIEPSTGQGY ERDPRSIARR AEEYLKSTGI GDTAFFGPEP EFFVFDEVKW DIDMSGARHT LIAEEAAWST GKDYESGNSG HRPRVKGGYF PVPPVDSAQD MRAEMCAKIE DIMGPGRVEV HHHEVASCQL EIGVSFNTLV RKADEVQQFK YAVWNVAHQY AKTATFMPKP MVGDNGSGMH VHMSISKDGK NLFAGDEYAG LSEMALYFIG GIIKHARALN AITNPSTNSY KRLVPHFEAP IMLAYSARNR SASIRIPYVS NPKGKRIEAR FPDPMMNPYL GFAALLMAGI DGIQNKIHPG EAADKNLYDL PPEEEAKIPT VAHSLDMALE ALQADHEFLL KGGVFTKEML DAYIELKTED VRRLNTTTHP VEFDMYYSL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA TGGCGAACAA GGTCCTTCAA CTCATACAAG AAAGTGGCGC AAAATGGGTC GATTTTCGCT TTACTGATAC TAAGGGTAAA GAACAGCACG TAACTTACCC AGCTGATTCT ATTGATGAAG ATACTTTTGA AGACGGTAAA ATGTTCGATG GTTCTTCAAT CGCTGGTTGG AAAGGTATTG AAGCATCTGA CATGATTTTA CGTCCGGATG CAGAAACTGG TTTCATCGAC CCGTTCTTTG CTGAGCCAAC AGTAGTTGTG ACTTGTGACG TTATTGAACC TTCAACTGGT CAAGGTTATG AACGTGACCC ACGTTCGATT GCTCGCCGTG CAGAAGAATA CTTAAAATCT ACAGGTATCG GTGATACTGC ATTCTTTGGT CCAGAGCCAG AATTCTTTGT ATTTGACGAA GTGAAATGGG ACATCGATAT GTCTGGCGCT CGTCATACAT TAATCGCTGA AGAGGCTGCT TGGTCTACTG GTAAAGATTA CGAGTCTGGT AACTCAGGTC ACCGTCCACG TGTTAAAGGT GGTTACTTCC CAGTTCCTCC AGTTGACTCT GCACAAGATA TGCGTGCAGA AATGTGTGCA AAAATTGAAG ACATTATGGG TCCAGGTCGT GTAGAAGTAC ACCACCACGA AGTTGCTTCT TGCCAATTAG AAATTGGTGT GAGCTTCAAT ACGCTTGTAC GTAAAGCTGA CGAAGTTCAA CAGTTCAAAT ATGCTGTTTG GAACGTTGCA CATCAATATG CAAAAACAGC TACGTTTATG CCTAAACCAA TGGTAGGTGA TAACGGTTCT GGTATGCACG TTCACATGTC TATCTCTAAA GATGGTAAGA ACTTGTTTGC TGGTGATGAA TATGCAGGCC TTTCAGAAAT GGCGTTGTAC TTCATCGGTG GTATCATCAA GCACGCTCGT GCATTGAATG CAATCACAAA CCCATCTACA AACTCATACA AGCGTTTGGT TCCTCACTTC GAAGCACCAA TTATGCTTGC TTACTCAGCG CGTAACCGTT CTGCGTCTAT CCGTATTCCT TACGTTTCTA ACCCTAAAGG CAAGCGTATC GAAGCTCGTT TCCCAGACCC AATGATGAAC CCGTACTTAG GTTTCGCTGC GTTATTAATG GCTGGTATCG ACGGTATCCA AAACAAGATC CATCCGGGCG AAGCTGCTGA CAAGAACTTG TACGATCTTC CTCCAGAAGA AGAAGCAAAA ATCCCAACAG TTGCTCATAG CCTAGATATG GCTCTTGAAG CACTTCAAGC AGATCACGAA TTCTTGTTAA AAGGTGGCGT GTTCACTAAA GAAATGCTTG ATGCATACAT CGAACTTAAA ACTGAAGATG TACGTCGTCT TAACACGACT ACTCACCCAG TTGAATTCGA TATGTACTAC AGCCTGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T