AcbaC.00097.a

pantoate--beta-alanine ligase

CENTER ID: AcbaC.00097.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00097.a.B2.GE38582 mature protein 20 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3229
GenBank: AKA32991.1
PGAT: acinetobacter:ABUW_3295
RefSeq: WP_000846362.1
UniProt: V5VGP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTETTIQGL AASLNPARAA RKIIGFVPTM GNLHEGHLTL VREAKKLCDV VVVSIFVNPT QFGPGEDFDN YPRTLEQDSR LLADVGCDII FAPSVEQMYG TQPRLTNISV SQITDDLCGS SRPGHFDGVA LVVTKLFNIV QPNYAFFGQK DYQQLAVIRQ FVQDLNIPLE VIGVPIVRAE DGLALSSRNG YLTPEQRQVA PVIYQGLKQA EEQLHQGKDL QQVLADLKTL LTDNGFVVDY VEARQPNLLA ASQFDRDIVL FVAAKLGGTR LIDNLQVAFT PQ
NT Sequence
ATGAAAACAG AAACCACTAT ACAAGGCTTA GCTGCTTCTT TAAATCCTGC TAGAGCTGCA CGTAAAATCA TTGGTTTTGT CCCAACGATG GGAAATCTAC ATGAAGGCCA CCTCACTCTT GTTCGTGAAG CGAAAAAGTT ATGTGATGTT GTGGTTGTGA GTATCTTCGT GAACCCAACA CAGTTTGGTC CGGGTGAAGA TTTTGATAAT TATCCGCGTA CGTTAGAGCA GGATAGTCGT CTACTTGCAG ATGTTGGCTG TGACATTATT TTTGCACCAT CAGTTGAACA AATGTACGGT ACACAACCTC GCTTAACTAA CATTAGCGTC AGTCAAATTA CTGATGATTT ATGTGGAAGC TCACGTCCGG GGCACTTTGA TGGCGTAGCA CTGGTTGTCA CCAAACTCTT TAATATTGTG CAACCGAATT ATGCTTTCTT TGGTCAAAAA GACTACCAGC AATTAGCAGT GATTCGCCAG TTTGTACAGG ATTTGAATAT TCCGTTAGAA GTTATTGGCG TACCAATTGT TCGTGCAGAA GATGGTTTGG CACTGAGTTC AAGAAATGGT TATTTGACTC CTGAACAGCG CCAGGTTGCA CCAGTCATTT ATCAAGGTTT AAAACAAGCC GAAGAGCAAT TACACCAAGG TAAAGATTTA CAACAAGTCT TGGCAGATTT GAAAACACTG TTGACAGACA ATGGTTTTGT TGTGGATTAT GTGGAGGCTC GCCAACCTAA TTTGTTAGCT GCTTCTCAAT TTGATCGTGA TATTGTGTTG TTTGTCGCGG CAAAATTAGG TGGAACGCGC TTAATTGATA ATCTACAAGT TGCCTTTACA CCTCAATAA
Details for AcbaC.00097.a.B2.GE38582
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA RKIIGFVPTM GNLHEGHLTL VREAKKLCDV VVVSIFVNPT QFGPGEDFDN YPRTLEQDSR LLADVGCDII FAPSVEQMYG TQPRLTNISV SQITDDLCGS SRPGHFDGVA LVVTKLFNIV QPNYAFFGQK DYQQLAVIRQ FVQDLNIPLE VIGVPIVRAE DGLALSSRNG YLTPEQRQVA PVIYQGLKQA EEQLHQGKDL QQVLADLKTL LTDNGFVVDY VEARQPNLLA ASQFDRDIVL FVAAKLGGTR LIDNLQVAFT PQ
Validated NT Sequence
atcttactca cttattgagg tgtaaaggca acttgtagat tatcaattaa gcgcgttcca cctaattttg ccgcgacaaa caacacaata tcacgatcaa attgagaagc agctaacaaa ttaggttggc gagcctccac ataatccaca acaaaaccat tgtctgtcaa cagtgttttc aaatctgcca agacttgttg taaatcttta ccttggtgta attgctcttc ggcttgtttt aaaccttgat aaatgactgg tgcaacctgg cgctgttcag gagtcaaata accatttctt gaactcagtg ccaaaccatc ttctgcacga acaattggta cgccaataac ttctaacgga atattcaaat cctgtacaaa ctggcgaatc actgctaatt gctggtagtc tttttgacca aagaaagcat aattcggttg cacaatatta aagagtttgg tgacaaccag tgctacgcca tcaaagtgcc ccggacgtga gcttccacat aaatcatcag taatttgact gacgctaatg ttagttaagc gaggttgtgt accgtacatt tgttcaactg atggtgcaaa aataatgtca cagccaacat ctgcaagtag acgactatcc tgctctaacg tacgcggata attatcaaaa tcttcacccg gaccaaactg tgttgggttc acgaagatac tcacaaccac aacatcacat aactttttcg cttcacgaac aagagtgagg tggccttcat gtagatttcc catcgttggg acaaaaccaa tgattttacg tgccatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHAR KIIGFVPTMG NLHEGHLTLV REAKKLCDVV VVSIFVNPTQ FGPGEDFDNY PRTLEQDSRL LADVGCDIIF APSVEQMYGT QPRLTNISVS QITDDLCGSS RPGHFDGVAL VVTKLFNIVQ PNYAFFGQKD YQQLAVIRQF VQDLNIPLEV IGVPIVRAED GLALSSRNGY LTPEQRQVAP VIYQGLKQAE EQLHQGKDLQ QVLADLKTLL TDNGFVVDYV EARQPNLLAA SQFDRDIVLF VAAKLGGTRL IDNLQVAFTP Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcacgta aaatcattgg ttttgtccca acgatgggaa atctacatga aggccacctc actcttgttc gtgaagcgaa aaagttatgt gatgttgtgg ttgtgagtat cttcgtgaac ccaacacagt ttggtccggg tgaagatttt gataattatc cgcgtacgtt agagcaggat agtcgtctac ttgcagatgt tggctgtgac attatttttg caccatcagt tgaacaaatg tacggtacac aacctcgctt aactaacatt agcgtcagtc aaattactga tgatttatgt ggaagctcac gtccggggca ctttgatggc gtagcactgg ttgtcaccaa actctttaat attgtgcaac cgaattatgc tttctttggt caaaaagact accagcaatt agcagtgatt cgccagtttg tacaggattt gaatattccg ttagaagtta ttggcgtacc aattgttcgt gcagaagatg gtttggcact gagttcaaga aatggttatt tgactcctga acagcgccag gttgcaccag tcatttatca aggtttaaaa caagccgaag agcaattaca ccaaggtaaa gatttacaac aagtcttggc agatttgaaa acactgttga cagacaatgg ttttgttgtg gattatgtgg aggctcgcca acctaatttg ttagctgctt ctcaatttga tcgtgatatt gtgttgtttg tcgcggcaaa attaggtgga acgcgcttaa ttgataatct acaagttgcc tttacacctc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat