SSGCID: Target Page

AcbaC.00084.a

phosphopyruvate hydratase (enolase)

CENTER ID:	AcbaC.00084.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00084.a.B1.GE38581	full length		1	429

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.1782
GenBank:	AKA31562.1
PGAT:	acinetobacter:ABUW_1827
RefSeq:	WP_000078452.1
UniProt:	V5VCM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQIVDIRAR EILDSRGNPT IEADVILESG VVGRACAPSG ASTGSREALE LRDGDKSRYL GKGVRTAVQN VNSSIHELLV GQSVFEQKAL DEKMIAFDGT ENKSKLGANA TLAVSLAAAH AAAAEQKLPL FQYIANLRGQ TTLTMPVPMM NILNGGAHAD NTVDIQEFMI EPVGFTSFAE ALRAGAEVFH SLKSVLKKQG LNTAVGDEGG FAPNLRSNEE AITVILQAIE QTGYKAGSDI MLALDCASSE FYKNGQYILE GEGNKSFTSN QFADYLAGLV KQYPIISIED GLDESDWEGW SYLTSILGDK IQLVGDDLFV TNPKILQRGI DEKVGNSILI KYNQIGTLTE TLDAIYLAKA NGYTTVISHR SGETEDSTIA DLAVGTAAGQ IKTGSLCRSD RVSKYNQLLR IEELTKAVYR GKAEFKGLN

NT Sequence

ATGAGCCAAA TCGTTGACAT TCGTGCACGT GAAATTTTGG ACTCTCGTGG TAACCCAACC ATCGAAGCAG ACGTCATCTT AGAATCTGGG GTGGTTGGTC GTGCATGTGC ACCATCTGGT GCTTCAACTG GTTCTCGTGA AGCTTTAGAA CTTCGTGACG GCGACAAATC TCGTTACTTA GGTAAAGGCG TTCGTACTGC TGTTCAGAAT GTAAATAGCT CAATTCATGA ATTGCTTGTT GGTCAAAGTG TTTTTGAACA AAAAGCGCTT GATGAAAAAA TGATTGCTTT TGATGGCACT GAAAACAAAT CTAAATTAGG TGCTAATGCA ACTTTGGCGG TATCTTTAGC AGCAGCTCAC GCAGCAGCAG CTGAACAAAA ACTTCCATTG TTTCAATACA TTGCTAACTT ACGTGGTCAA ACTACTTTGA CTATGCCTGT ACCAATGATG AACATTTTGA ACGGTGGTGC TCACGCAGAT AACACTGTTG ATATTCAAGA GTTCATGATT GAGCCAGTTG GTTTCACTTC ATTCGCTGAA GCATTACGTG CTGGTGCAGA AGTATTCCAT TCTTTGAAAT CAGTTCTTAA GAAACAAGGT TTGAACACAG CTGTAGGTGA TGAAGGTGGT TTTGCGCCTA ACCTTCGTTC AAATGAAGAA GCGATTACAG TAATTCTTCA AGCAATTGAG CAAACTGGTT ATAAAGCTGG TTCAGATATT ATGCTTGCAC TTGACTGTGC ATCTTCAGAA TTCTACAAGA ATGGTCAATA TATTCTTGAA GGTGAAGGCA ACAAGTCTTT TACAAGCAAT CAATTTGCTG ATTACTTAGC TGGTCTTGTT AAGCAGTATC CAATTATCTC TATTGAAGAT GGTTTGGATG AGTCTGACTG GGAAGGCTGG AGTTACTTAA CTTCAATTCT TGGTGATAAA ATCCAATTGG TTGGTGACGA TTTATTCGTA ACTAATCCTA AGATTTTACA ACGTGGTATT GATGAAAAAG TTGGTAACTC AATTTTGATC AAATATAACC AGATTGGTAC ATTGACTGAA ACTTTAGATG CAATCTACCT TGCTAAAGCA AATGGTTACA CTACTGTTAT TTCTCACCGT TCTGGTGAAA CAGAAGATTC AACTATTGCT GATTTAGCAG TAGGTACAGC AGCAGGTCAA ATTAAGACTG GTTCACTTTG CCGTTCTGAC CGTGTTTCTA AATATAACCA ATTGCTTCGT ATTGAAGAGT TAACTAAAGC GGTTTACCGC GGTAAAGCTG AATTCAAAGG TTTAAACTAA

Details for AcbaC.00084.a.B1.GE38581

HARVESTED ON:	11/19/2014
SEQUENCED ON:	12/10/2014
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS QIVDIRAREI LDSRGNPTIE ADVILESGVV GRACAPSGAS TGSREALELR DGDKSRYLGK GVRTAVQNVN SSIHELLVGQ SVFEQKALDE KMIAFDGTEN KSKLGANATL AVSLAAAHAA AAEQKLPLFQ YIANLRGQTT LTMPVPMMNI LNGGAHADNT VDIQEFMIEP VGFTSFAEAL RAGAEVFHSL KSVLKKQGLN TAVGDEGGFA PNLRSNEEAI TVILQAIEQT GYKAGSDIML ALDCASSEFY KNGQYILEGE GNKSFTSNQF ADYLAGLVKQ YPIISIEDGL DESDWEGWSY LTSILGDKIQ LVGDDLFVTN PKILQRGIDE KVGNSILIKY NQIGTLTETL DAIYLAKANG YTTVISHRSG ETEDSTIADL AVGTAAGQIK TGSLCRSDRV SKYNQLLRIE ELTKAVYRG

Validated NT Sequence

ttaccgcggt aaaccgcttt agttaactct tcaatacgaa gcaattggtt atatttagaa acacggtcag aacggcaaag tgaaccagtc ttaatttgac ctgctgctgt acctactgct aaatcagcaa tagttgaatc ttctgtttca ccagaacggt gagaaataac agtagtgtaa ccatttgctt tagcaaggta gattgcatct aaagtttcag tcaatgtacc aatctggtta tatttgatca aaattgagtt accaactttt tcatcaatac cacgttgtaa aatcttagga ttagttacga ataaatcgtc accaaccaat tggattttat caccaagaat tgaagttaag taactccagc cttcccagtc agactcatcc aaaccatctt caatagagat aattggatac tgcttaacaa gaccagctaa gtaatcagca aattgattgc ttgtaaaaga cttgttgcct tcaccttcaa gaatatattg accattcttg tagaattctg aagatgcaca gtcaagtgca agcataatat ctgaaccagc tttataacca gtttgctcaa ttgcttgaag aattactgta atcgcttctt catttgaacg aaggttaggc gcaaaaccac cttcatcacc tacagctgtg ttcaaacctt gtttcttaag aactgatttc aaagaatgga atacttctgc accagcacgt aatgcttcag cgaatgaagt gaaaccaact ggctcaatca tgaactcttg aatatcaaca gtgttatctg cgtgagcacc accgttcaaa atgttcatca ttggtacagg catagtcaaa gtagtttgac cacgtaagtt agcaatgtat tgaaacaatg gaagtttttg ttcagctgct gctgcgtgag ctgctgctaa agataccgcc aaagttgcat tagcacctaa tttagatttg ttttcagtgc catcaaaagc aatcattttt tcatcaagcg ctttttgttc aaaaacactt tgaccaacaa gcaattcatg aattgagcta tttacattct gaacagcagt acgaacgcct ttacctaagt aacgagattt gtcgccgtca cgaagttcta aagcttcacg agaaccagtt gaagcaccag atggtgcaca tgcacgacca accaccccag attctaagat gacgtctgct tcgatggttg ggttaccacg agagtccaaa atttcacgtg cacgaatgtc aacgatttgg ctcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aaatcgttga cattcgtgca cgtgaaattt tggactctcg tggtaaccca accatcgaag cagacgtcat cttagaatct ggggtggttg gtcgtgcatg tgcaccatct ggtgcttcaa ctggttctcg tgaagcttta gaacttcgtg acggcgacaa atctcgttac ttaggtaaag gcgttcgtac tgctgttcag aatgtaaata gctcaattca tgaattgctt gttggtcaaa gtgtttttga acaaaaagcg cttgatgaaa aaatgattgc ttttgatggc actgaaaaca aatctaaatt aggtgctaat gcaactttgg cggtatcttt agcagcagct cacgcagcag cagctgaaca aaaacttcca ttgtttcaat acattgctaa cttacgtggt caaactactt tgactatgcc tgtaccaatg atgaacattt tgaacggtgg tgctcacgca gataacactg ttgatattca agagttcatg attgagccag ttggtttcac ttcattcgct gaagcattac gtgctggtgc agaagtattc cattctttga aatcagttct taagaaacaa ggtttgaaca cagctgtagg tgatgaaggt ggttttgcgc ctaaccttcg ttcaaatgaa gaagcgatta cagtaattct tcaagcaatt gagcaaactg gttataaagc tggttcagat attatgcttg cacttgactg tgcatcttca gaattctaca agaatggtca atatattctt gaaggtgaag gcaacaagtc ttttacaagc aatcaatttg ctgattactt agctggtctt gttaagcagt atccaattat ctctattgaa gatggtttgg atgagtctga ctgggaaggc tggagttact taacttcaat tcttggtgat aaaatccaat tggttggtga cgatttattc gtaactaatc ctaagatttt acaacgtggt attgatgaaa aagttggtaa ctcaattttg atcaaatata accagattgg tacattgact gaaactttag atgcaatcta ccttgctaaa gcaaatggtt acactactgt tatttctcac cgttctggtg aaacagaaga ttcaactatt gctgatttag cagtaggtac agcagcaggt caaattaaga ctggttcact ttgccgttct gaccgtgttt ctaaatataa ccaattgctt cgtattgaag agttaactaa agcggtttac cgcggtaaag ctgaattcaa aggtttaaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat