AcbaC.00082.a

porphobilinogen deaminase

CENTER ID: AcbaC.00082.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00082.a.B1.GE38580 full length 1 314
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3541
GenBank: AKA33300.1
PGAT: acinetobacter:ABUW_3614
UniProt: A0A0R4J824

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
LFDQPMLEFM KTLKIATRQS PLALWQAEHI RARLQERHPD LTVELVKFVT QGDKILDTPL AKIGGKGLFV KELEAALLDG RADLAVHSMK DVPMALPEGL TLAVICERED PLDAFVSNQF EKFADLPQGA KVGTSSLRRK SQILKQRPDL QIIDLRGNVG TRLAKLDDGQ YDAIILASAG LKRLGLAERI RHCIEPSVSL PAVGQGALGL ECRADDQEVL ALIQPLLHPE TDVCVRAERA FNAYLEGGCQ VPIAGYATLQ DGKIHIEGRV GSVDGQTLLR AELTDEANNA QQLGENLARN LLDQGAGDLL KALY
NT Sequence
TTGTTTGACC AGCCCATGCT TGAATTTATG AAAACCTTGA AAATTGCTAC ACGACAAAGC CCACTTGCTC TTTGGCAGGC AGAACATATT CGCGCCCGTT TACAGGAGCG TCACCCAGAT TTAACGGTGG AGTTGGTTAA GTTTGTCACG CAGGGCGATA AAATTTTAGA TACACCTTTA GCAAAAATTG GTGGTAAAGG GTTGTTCGTT AAAGAATTGG AAGCAGCACT TTTAGATGGT CGTGCGGACC TTGCTGTACA TTCAATGAAA GATGTTCCGA TGGCATTACC AGAAGGTTTA ACTTTGGCCG TAATTTGCGA ACGTGAAGAT CCGTTAGATG CTTTTGTTTC GAATCAATTT GAAAAATTTG CTGATTTGCC GCAAGGAGCA AAAGTTGGCA CCTCAAGTTT ACGTCGTAAA TCACAAATTT TAAAACAACG TCCAGATCTA CAAATTATTG ATTTACGTGG TAATGTCGGT ACCCGCTTAG CGAAGCTAGA CGATGGTCAA TATGATGCCA TTATCTTGGC GAGTGCTGGT TTAAAACGTT TAGGATTGGC AGAACGCATT CGCCACTGTA TCGAGCCAAG TGTAAGTTTG CCTGCGGTTG GTCAGGGCGC TCTTGGTTTG GAATGCCGTG CAGATGATCA AGAAGTGTTG GCTTTAATTC AGCCACTTCT ACACCCAGAG ACAGATGTAT GTGTACGTGC TGAGCGAGCA TTCAATGCTT ATCTTGAAGG TGGTTGTCAG GTGCCGATTG CAGGTTATGC AACACTCCAA GATGGTAAAA TTCATATTGA AGGCCGTGTA GGTAGTGTTG ATGGGCAAAC ATTATTACGG GCTGAGCTTA CAGATGAAGC GAATAATGCT CAGCAATTAG GTGAAAACCT TGCTCGGAAC TTACTTGATC AAGGCGCTGG TGATTTATTA AAGGCCCTCT ACTAA
Details for AcbaC.00082.a.B1.GE38580
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 35kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML FDQPMLEFMK TLKIATRQSP LALWQAEHIR ARLQERHPDL TVELVKFVTQ GDKILDTPLA KIGGKGLFVK ELEAALLDGR ADLAVHSMKD VPMALPEGLT LAVICEREDP LDAFVSNQFE KFADLPQGAK VGTSSLRRKS QILKQRPDLQ IIDLRGNVGT HLAKLDDGQY DAIILASAGL KRLGLAERIR HCIEPSVSLP AVGQGALGLE CRADDQEVLA LIQPLLHPET DVCVRAERAF NAYLEGGCQV PIAGYATLQD GKIHIEGRVG SVDGQTLLRA ELTDEANNAQ QLGENLARNL LDQGAXXXLK ALY
Validated NT Sequence
ttactcactt agtagagggc ctttaannnn nnnncagcgc cttgatcaag taagttccga gcaaggtttt cacctaattg ctgagcatta ttcgcttcat ctgtaagctc agcccgtaat aatgtttgcc catcaacact acctacacgg ccttcaatat gaattttacc atcttggagt gttgcataac ctgcaatcgg cacctgacaa ccaccttcaa gataagcatt gaatgctcgc tcagcacgta cacatacatc tgtctctggg tgtagaagtg gctgaattaa agccaacact tcttgatcat ctgcacggca ttccaaacca agagcgccct gaccaaccgc aggcaaactt acacttggct cgatacagtg gcgaatgcgt tctgccaatc ctaaacgttt taaaccagca ctcgccaaga taatggcatc atattgacca tcgtctagct tcgctaagtg ggtaccgaca ttaccacgta aatcaataat ttgtagatct ggacgttgtt ttaaaatttg tgatttacga cgtaaacttg aggtgccaac ttttgctcct tgcggcaaat cagcaaattt ttcaaattga ttcgaaacaa aagcatctaa cggatcttca cgttcgcaaa ttacggccaa agttaaacct tctggtaatg ccatcggaac atctttcatt gaatgtacag caaggtccgc acgaccatct aaaagtgctg cttccaattc tttaacgaac aaccctttac caccaatttt tgctaaaggt gtatctaaaa ttttatcgcc ctgcgtgaca aacttaacca actccaccgt taaatctggg tgacgctcct gtaaacgggc gcgaatatgt tctgcctgcc aaagagcaag tgggctttgt cgtgtagcaa ttttcaaggt tttcataaat tcaagcatgg gctggtcaaa caacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHLF DQPMLEFMKT LKIATRQSPL ALWQAEHIRA RLQERHPDLT VELVKFVTQG DKILDTPLAK IGGKGLFVKE LEAALLDGRA DLAVHSMKDV PMALPEGLTL AVICEREDPL DAFVSNQFEK FADLPQGAKV GTSSLRRKSQ ILKQRPDLQI IDLRGNVGTR LAKLDDGQYD AIILASAGLK RLGLAERIRH CIEPSVSLPA VGQGALGLEC RADDQEVLAL IQPLLHPETD VCVRAERAFN AYLEGGCQVP IAGYATLQDG KIHIEGRVGS VDGQTLLRAE LTDEANNAQQ LGENLARNLL DQGAGDLLKA LY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtttg accagcccat gcttgaattt atgaaaacct tgaaaattgc tacacgacaa agcccacttg ctctttggca ggcagaacat attcgcgccc gtttacagga gcgtcaccca gatttaacgg tggagttggt taagtttgtc acgcagggcg ataaaatttt agatacacct ttagcaaaaa ttggtggtaa agggttgttc gttaaagaat tggaagcagc acttttagat ggtcgtgcgg accttgctgt acattcaatg aaagatgttc cgatggcatt accagaaggt ttaactttgg ccgtaatttg cgaacgtgaa gatccgttag atgcttttgt ttcgaatcaa tttgaaaaat ttgctgattt gccgcaagga gcaaaagttg gcacctcaag tttacgtcgt aaatcacaaa ttttaaaaca acgtccagat ctacaaatta ttgatttacg tggtaatgtc ggtacccgct tagcgaagct agacgatggt caatatgatg ccattatctt ggcgagtgct ggtttaaaac gtttaggatt ggcagaacgc attcgccact gtatcgagcc aagtgtaagt ttgcctgcgg ttggtcaggg cgctcttggt ttggaatgcc gtgcagatga tcaagaagtg ttggctttaa ttcagccact tctacaccca gagacagatg tatgtgtacg tgctgagcga gcattcaatg cttatcttga aggtggttgt caggtgccga ttgcaggtta tgcaacactc caagatggta aaattcatat tgaaggccgt gtaggtagtg ttgatgggca aacattatta cgggctgagc ttacagatga agcgaataat gctcagcaat taggtgaaaa ccttgctcgg aacttacttg atcaaggcgc tggtgattta ttaaaggccc tctactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat