AcbaC.00063.a
histidyl-tRNA synthetase
| CENTER ID: | AcbaC.00063.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00063.a.B1.GE38578 | full length | 1 | 430 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00063.a.B1.PW37682 | full length | 1 | 430 |
Structures
5E3I
DEPOSITED: 10/2/2015
DETERMINATION: XRay
CLONE: AcbaC.00063.a.B1.GE38578
PROTEIN: AcbaC.00063.a.B1.PW37682
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA33072.1 |
| PATRIC ID: | fig|470.1345.peg.3311 |
| PGAT: | acinetobacter:ABUW_3376 |
| RefSeq: | WP_000095254.1 |
| UniProt: | A0A086HW61 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSIVAIKGF NDVLPTQTAA WRRLEQHLAS LMDAYGYQQI RLPIVEQTGL FKRAIGDATD IVEKEMYTFF DKGNPPESLT LRPEGTAGCV RALVEHNLLR GATPRVWYMG PMFRYEKPQK GRYRQFHQFG VETFGVATPD IDAELIMLTA RLWKRMGVDH MVQLELNTLG ETDERTEYRN ALVAFLNEHK DALDEDSQRR LTTNPLRILD SKIESTQKIL ENAPKLHDFL KEDSLSHFQQ LQDYLTAAGI KFVINQKLVR GLDYYNKTVF EWTTTALGSQ GTVCAGGRYD GLVGQLKGKA DQSVPAVGFA MGMERLLLLL EQVEQAEIVR DCEAFLVAEP AYQSKALVLA EQLRDQLEAA NSNIRIKTGS QGSMKSQMKK ADQAGAVYAI ILGEREWEAQ QLAVKELATA EQSQVALAEL VPFLIEKFTK
NT Sequence
ATGAGTTCAA TTGTCGCAAT CAAAGGTTTT AACGACGTTT TACCAACGCA AACAGCAGCG TGGAGACGTC TCGAGCAGCA TTTAGCATCT TTAATGGATG CTTATGGTTA CCAACAAATT CGTTTGCCGA TCGTTGAACA AACGGGACTG TTTAAACGTG CTATTGGTGA TGCTACCGAT ATCGTAGAAA AAGAAATGTA CACATTCTTC GATAAAGGAA ACCCACCTGA ATCACTGACT TTACGTCCGG AAGGAACAGC GGGTTGTGTT CGTGCGTTGG TTGAACATAA CTTGTTGCGT GGTGCTACGC CACGTGTGTG GTATATGGGA CCTATGTTCC GTTATGAAAA ACCACAAAAA GGCCGTTACC GCCAGTTCCA CCAGTTTGGT GTGGAAACTT TTGGTGTAGC GACTCCTGAT ATCGACGCTG AATTGATTAT GTTGACTGCC CGTTTGTGGA AGCGTATGGG TGTTGATCAC ATGGTTCAAC TTGAGTTGAA TACTTTGGGT GAAACAGATG AGCGTACGGA ATATCGTAAT GCTTTGGTTG CGTTCTTAAA TGAACATAAA GATGCTTTGG ATGAAGATTC ACAGCGCCGT TTAACAACAA ACCCATTACG TATTTTAGAT TCTAAAATTG AGTCGACTCA GAAAATTCTG GAAAATGCGC CGAAATTACA TGACTTCTTA AAAGAAGACA GTTTAAGCCA CTTCCAACAA TTACAAGATT ATTTGACTGC TGCAGGCATT AAATTTGTAA TTAACCAAAA ATTGGTACGT GGTCTAGATT ATTACAATAA AACTGTTTTT GAATGGACAA CAACAGCATT AGGTTCGCAA GGTACAGTAT GTGCAGGTGG ACGCTATGAT GGTTTGGTTG GCCAGTTAAA AGGTAAGGCT GATCAGTCTG TTCCAGCGGT CGGTTTTGCA ATGGGGATGG AGCGTTTATT GCTTCTACTT GAGCAAGTCG AACAAGCTGA AATTGTACGT GACTGTGAAG CTTTCTTAGT TGCGGAGCCT GCTTATCAAT CTAAAGCTTT AGTTTTGGCA GAACAATTAC GTGATCAGTT AGAAGCTGCG AATAGTAACA TCCGCATTAA AACTGGTTCA CAAGGCAGCA TGAAGAGTCA GATGAAAAAA GCTGATCAGG CTGGGGCTGT TTACGCCATA ATTTTGGGTG AACGTGAGTG GGAAGCACAG CAGCTTGCTG TAAAAGAATT GGCAACAGCT GAGCAATCAC AAGTTGCATT GGCTGAACTC GTACCATTCT TAATTGAAAA ATTTACAAAA TAA
Details for AcbaC.00063.a.B1.GE38578
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS SIVAIKGFND VLPTQTAAWR RLEQHLASLM DAYGYQQIRL PIVEQTGLFK RAIGDATDIV EKEMYTFFDK GNPPESLTLR PEGTAGCVRA LVEHNLLRGA TPRVWYMGPM FRYEKPQKGR YRQFHQFGVE TFGVATPDID AELIMLTARL WKRMGVDHMV QLELNTLGET DERTEYRNAL VAFLNEHKDA LDEDSQRRLT TNPLRILDSK IESTQKILEN APKLHDFLKE DSLSHFQQLQ DYLTAAGIKF VINQKLVRGL DYYNKTVFEW TTTALGSQGT VCAGGRYDGL VGQLKGKADQ SVPAVGFAMG MERLLLLLEQ VEQAEIVRDC EAFLVAEPAY QSKALVLAEQ LRDQLEAANS NIRIKTGSQG SMKSQMKKAD QAGAVYAIIL GEREWEAQQL AVKELATAEQ SQVALAELV
Validated NT Sequence
tggtacgagt tcagccaatg caacttgtga ttgctcagct gttgccaatt cttttacagc aagctgctgt gcttcccact cacgttcacc caaaattatg gcgtaaacag ccccagcctg atcagctttt ttcatctgac tcttcatgct gccttgtgaa ccagttttaa tgcggatgtt actattcgca gcttctaact gatcacgtaa ttgttctgcc aaaactaaag ctttagattg ataagcaggc tccgcaacta agaaagcttc acagtcacgt acaatttcag cttgttcgac ttgctcaagt agaagcaata aacgctccat ccccattgca aaaccgaccg ctggaacaga ctgatcagcc ttacctttta actggccaac caaaccatca tagcgtccac ctgcacatac tgtaccttgc gaacctaatg ctgttgttgt ccattcaaaa acagttttat tgtaataatc tagaccacgt accaattttt ggttaattac aaatttaatg cctgcagcag tcaaataatc ttgtaattgt tggaagtggc ttaaactgtc ttcttttaag aagtcatgta atttcggcgc attttccaga attttctgag tcgactcaat tttagaatct aaaatacgta atgggtttgt tgttaaacgg cgctgtgaat cttcatccaa agcatcttta tgttcattta agaacgcaac caaagcatta cgatattccg tacgctcatc tgtttcaccc aaagtattca actcaagttg aaccatgtga tcaacaccca tacgcttcca caaacgggca gtcaacataa tcaattcagc gtcgatatca ggagtcgcta caccaaaagt ttccacacca aactggtgga actggcggta acggcctttt tgtggttttt cataacggaa cataggtccc atataccaca cacgtggcgt agcaccacgc aacaagttat gttcaaccaa cgcacgaaca caacccgctg ttccttccgg acgtaaagtc agtgattcag gtgggtttcc tttatcgaag aatgtgtaca tttctttttc tacgatatcg gtagcatcac caatagcacg tttaaacagt cccgtttgtt caacgatcgg caaacgaatt tgttggtaac cataagcatc cattaaagat gctaaatgct gctcgagacg tctccacgct gctgtttgcg ttggtaaaac gtcgttaaaa cctttgattg cgacaattga actcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS SIVAIKGFND VLPTQTAAWR RLEQHLASLM DAYGYQQIRL PIVEQTGLFK RAIGDATDIV EKEMYTFFDK GNPPESLTLR PEGTAGCVRA LVEHNLLRGA TPRVWYMGPM FRYEKPQKGR YRQFHQFGVE TFGVATPDID AELIMLTARL WKRMGVDHMV QLELNTLGET DERTEYRNAL VAFLNEHKDA LDEDSQRRLT TNPLRILDSK IESTQKILEN APKLHDFLKE DSLSHFQQLQ DYLTAAGIKF VINQKLVRGL DYYNKTVFEW TTTALGSQGT VCAGGRYDGL VGQLKGKADQ SVPAVGFAMG MERLLLLLEQ VEQAEIVRDC EAFLVAEPAY QSKALVLAEQ LRDQLEAANS NIRIKTGSQG SMKSQMKKAD QAGAVYAIIL GEREWEAQQL AVKELATAEQ SQVALAELVP FLIEKFTK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt caattgtcgc aatcaaaggt tttaacgacg ttttaccaac gcaaacagca gcgtggagac gtctcgagca gcatttagca tctttaatgg atgcttatgg ttaccaacaa attcgtttgc cgatcgttga acaaacggga ctgtttaaac gtgctattgg tgatgctacc gatatcgtag aaaaagaaat gtacacattc ttcgataaag gaaacccacc tgaatcactg actttacgtc cggaaggaac agcgggttgt gttcgtgcgt tggttgaaca taacttgttg cgtggtgcta cgccacgtgt gtggtatatg ggacctatgt tccgttatga aaaaccacaa aaaggccgtt accgccagtt ccaccagttt ggtgtggaaa cttttggtgt agcgactcct gatatcgacg ctgaattgat tatgttgact gcccgtttgt ggaagcgtat gggtgttgat cacatggttc aacttgagtt gaatactttg ggtgaaacag atgagcgtac ggaatatcgt aatgctttgg ttgcgttctt aaatgaacat aaagatgctt tggatgaaga ttcacagcgc cgtttaacaa caaacccatt acgtatttta gattctaaaa ttgagtcgac tcagaaaatt ctggaaaatg cgccgaaatt acatgacttc ttaaaagaag acagtttaag ccacttccaa caattacaag attatttgac tgctgcaggc attaaatttg taattaacca aaaattggta cgtggtctag attattacaa taaaactgtt tttgaatgga caacaacagc attaggttcg caaggtacag tatgtgcagg tggacgctat gatggtttgg ttggccagtt aaaaggtaag gctgatcagt ctgttccagc ggtcggtttt gcaatgggga tggagcgttt attgcttcta cttgagcaag tcgaacaagc tgaaattgta cgtgactgtg aagctttctt agttgcggag cctgcttatc aatctaaagc tttagttttg gcagaacaat tacgtgatca gttagaagct gcgaatagta acatccgcat taaaactggt tcacaaggca gcatgaagag tcagatgaaa aaagctgatc aggctggggc tgtttacgcc ataattttgg gtgaacgtga gtgggaagca cagcagcttg ctgtaaaaga attggcaaca gctgagcaat cacaagttgc attggctgaa ctcgtaccat tcttaattga aaaatttaca aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for AcbaC.00063.a.B1.PW37682
| PURIFICATION DATe: | 3/25/2015 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS SIVAIKGFND VLPTQTAAWR RLEQHLASLM DAYGYQQIRL PIVEQTGLFK RAIGDATDIV EKEMYTFFDK GNPPESLTLR PEGTAGCVRA LVEHNLLRGA TPRVWYMGPM FRYEKPQKGR YRQFHQFGVE TFGVATPDID AELIMLTARL WKRMGVDHMV QLELNTLGET DERTEYRNAL VAFLNEHKDA LDEDSQRRLT TNPLRILDSK IESTQKILEN APKLHDFLKE DSLSHFQQLQ DYLTAAGIKF VINQKLVRGL DYYNKTVFEW TTTALGSQGT VCAGGRYDGL VGQLKGKADQ SVPAVGFAMG MERLLLLLEQ VEQAEIVRDC EAFLVAEPAY QSKALVLAEQ LRDQLEAANS NIRIKTGSQG SMKSQMKKAD QAGAVYAIIL GEREWEAQQL AVKELATAEQ SQVALAELV
Validated NT Sequence
tggtacgagt tcagccaatg caacttgtga ttgctcagct gttgccaatt cttttacagc aagctgctgt gcttcccact cacgttcacc caaaattatg gcgtaaacag ccccagcctg atcagctttt ttcatctgac tcttcatgct gccttgtgaa ccagttttaa tgcggatgtt actattcgca gcttctaact gatcacgtaa ttgttctgcc aaaactaaag ctttagattg ataagcaggc tccgcaacta agaaagcttc acagtcacgt acaatttcag cttgttcgac ttgctcaagt agaagcaata aacgctccat ccccattgca aaaccgaccg ctggaacaga ctgatcagcc ttacctttta actggccaac caaaccatca tagcgtccac ctgcacatac tgtaccttgc gaacctaatg ctgttgttgt ccattcaaaa acagttttat tgtaataatc tagaccacgt accaattttt ggttaattac aaatttaatg cctgcagcag tcaaataatc ttgtaattgt tggaagtggc ttaaactgtc ttcttttaag aagtcatgta atttcggcgc attttccaga attttctgag tcgactcaat tttagaatct aaaatacgta atgggtttgt tgttaaacgg cgctgtgaat cttcatccaa agcatcttta tgttcattta agaacgcaac caaagcatta cgatattccg tacgctcatc tgtttcaccc aaagtattca actcaagttg aaccatgtga tcaacaccca tacgcttcca caaacgggca gtcaacataa tcaattcagc gtcgatatca ggagtcgcta caccaaaagt ttccacacca aactggtgga actggcggta acggcctttt tgtggttttt cataacggaa cataggtccc atataccaca cacgtggcgt agcaccacgc aacaagttat gttcaaccaa cgcacgaaca caacccgctg ttccttccgg acgtaaagtc agtgattcag gtgggtttcc tttatcgaag aatgtgtaca tttctttttc tacgatatcg gtagcatcac caatagcacg tttaaacagt cccgtttgtt caacgatcgg caaacgaatt tgttggtaac cataagcatc cattaaagat gctaaatgct gctcgagacg tctccacgct gctgtttgcg ttggtaaaac gtcgttaaaa cctttgattg cgacaattga actcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS SIVAIKGFND VLPTQTAAWR RLEQHLASLM DAYGYQQIRL PIVEQTGLFK RAIGDATDIV EKEMYTFFDK GNPPESLTLR PEGTAGCVRA LVEHNLLRGA TPRVWYMGPM FRYEKPQKGR YRQFHQFGVE TFGVATPDID AELIMLTARL WKRMGVDHMV QLELNTLGET DERTEYRNAL VAFLNEHKDA LDEDSQRRLT TNPLRILDSK IESTQKILEN APKLHDFLKE DSLSHFQQLQ DYLTAAGIKF VINQKLVRGL DYYNKTVFEW TTTALGSQGT VCAGGRYDGL VGQLKGKADQ SVPAVGFAMG MERLLLLLEQ VEQAEIVRDC EAFLVAEPAY QSKALVLAEQ LRDQLEAANS NIRIKTGSQG SMKSQMKKAD QAGAVYAIIL GEREWEAQQL AVKELATAEQ SQVALAELVP FLIEKFTK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT CAATTGTCGC AATCAAAGGT TTTAACGACG TTTTACCAAC GCAAACAGCA GCGTGGAGAC GTCTCGAGCA GCATTTAGCA TCTTTAATGG ATGCTTATGG TTACCAACAA ATTCGTTTGC CGATCGTTGA ACAAACGGGA CTGTTTAAAC GTGCTATTGG TGATGCTACC GATATCGTAG AAAAAGAAAT GTACACATTC TTCGATAAAG GAAACCCACC TGAATCACTG ACTTTACGTC CGGAAGGAAC AGCGGGTTGT GTTCGTGCGT TGGTTGAACA TAACTTGTTG CGTGGTGCTA CGCCACGTGT GTGGTATATG GGACCTATGT TCCGTTATGA AAAACCACAA AAAGGCCGTT ACCGCCAGTT CCACCAGTTT GGTGTGGAAA CTTTTGGTGT AGCGACTCCT GATATCGACG CTGAATTGAT TATGTTGACT GCCCGTTTGT GGAAGCGTAT GGGTGTTGAT CACATGGTTC AACTTGAGTT GAATACTTTG GGTGAAACAG ATGAGCGTAC GGAATATCGT AATGCTTTGG TTGCGTTCTT AAATGAACAT AAAGATGCTT TGGATGAAGA TTCACAGCGC CGTTTAACAA CAAACCCATT ACGTATTTTA GATTCTAAAA TTGAGTCGAC TCAGAAAATT CTGGAAAATG CGCCGAAATT ACATGACTTC TTAAAAGAAG ACAGTTTAAG CCACTTCCAA CAATTACAAG ATTATTTGAC TGCTGCAGGC ATTAAATTTG TAATTAACCA AAAATTGGTA CGTGGTCTAG ATTATTACAA TAAAACTGTT TTTGAATGGA CAACAACAGC ATTAGGTTCG CAAGGTACAG TATGTGCAGG TGGACGCTAT GATGGTTTGG TTGGCCAGTT AAAAGGTAAG GCTGATCAGT CTGTTCCAGC GGTCGGTTTT GCAATGGGGA TGGAGCGTTT ATTGCTTCTA CTTGAGCAAG TCGAACAAGC TGAAATTGTA CGTGACTGTG AAGCTTTCTT AGTTGCGGAG CCTGCTTATC AATCTAAAGC TTTAGTTTTG GCAGAACAAT TACGTGATCA GTTAGAAGCT GCGAATAGTA ACATCCGCAT TAAAACTGGT TCACAAGGCA GCATGAAGAG TCAGATGAAA AAAGCTGATC AGGCTGGGGC TGTTTACGCC ATAATTTTGG GTGAACGTGA GTGGGAAGCA CAGCAGCTTG CTGTAAAAGA ATTGGCAACA GCTGAGCAAT CACAAGTTGC ATTGGCTGAA CTCGTACCAT TCTTAATTGA AAAATTTACA AAATGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT