AcbaC.00063.a
histidyl-tRNA synthetase
CENTER ID:
AcbaC.00063.a
ORGANISM:
Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
AcbaC.00063.a.B1.GE38578
full length
info
1
430
AcbaC.00063.a.B1.GE38578
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
AcbaC.00063.a.B1.PW37682
full length
info
1
430
PW37682
Structures
5E3I
DEPOSITED: 10/2/2015
DETERMINATION: XRay
CLONE: AcbaC.00063.a.B1.GE38578
PROTEIN: AcbaC.00063.a.B1.PW37682
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PDB:
4YL5 ,
4YWR ,
4ZIY ,
4ZJU ,
4ZN6 ,
4ZR8 ,
5BT8 ,
5CC8 ,
5CM7 ,
5CY4 ,
5DD7 ,
5E3I ,
5EKS ,
5EO6 ,
5HA4 ,
5IDV ,
5J3B ,
5KHA ,
5U7S ,
5UDF ,
5URB ,
5VMB ,
5VMK ,
5VPU ,
6AMY ,
6AMZ ,
6BNG ,
6BQY ,
6BQZ ,
6C49 ,
6CAU ,
6D6K ,
6MFM ,
6MPR ,
6MRF
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSIVAIKGF NDVLPTQTAA WRRLEQHLAS LMDAYGYQQI RLPIVEQTGL FKRAIGDATD IVEKEMYTFF DKGNPPESLT LRPEGTAGCV RALVEHNLLR GATPRVWYMG PMFRYEKPQK GRYRQFHQFG VETFGVATPD IDAELIMLTA RLWKRMGVDH MVQLELNTLG ETDERTEYRN ALVAFLNEHK DALDEDSQRR LTTNPLRILD SKIESTQKIL ENAPKLHDFL KEDSLSHFQQ LQDYLTAAGI KFVINQKLVR GLDYYNKTVF EWTTTALGSQ GTVCAGGRYD GLVGQLKGKA DQSVPAVGFA MGMERLLLLL EQVEQAEIVR DCEAFLVAEP AYQSKALVLA EQLRDQLEAA NSNIRIKTGS QGSMKSQMKK ADQAGAVYAI ILGEREWEAQ QLAVKELATA EQSQVALAEL VPFLIEKFTK
NT Sequence
ATGAGTTCAA TTGTCGCAAT CAAAGGTTTT AACGACGTTT TACCAACGCA AACAGCAGCG TGGAGACGTC TCGAGCAGCA TTTAGCATCT TTAATGGATG CTTATGGTTA CCAACAAATT CGTTTGCCGA TCGTTGAACA AACGGGACTG TTTAAACGTG CTATTGGTGA TGCTACCGAT ATCGTAGAAA AAGAAATGTA CACATTCTTC GATAAAGGAA ACCCACCTGA ATCACTGACT TTACGTCCGG AAGGAACAGC GGGTTGTGTT CGTGCGTTGG TTGAACATAA CTTGTTGCGT GGTGCTACGC CACGTGTGTG GTATATGGGA CCTATGTTCC GTTATGAAAA ACCACAAAAA GGCCGTTACC GCCAGTTCCA CCAGTTTGGT GTGGAAACTT TTGGTGTAGC GACTCCTGAT ATCGACGCTG AATTGATTAT GTTGACTGCC CGTTTGTGGA AGCGTATGGG TGTTGATCAC ATGGTTCAAC TTGAGTTGAA TACTTTGGGT GAAACAGATG AGCGTACGGA ATATCGTAAT GCTTTGGTTG CGTTCTTAAA TGAACATAAA GATGCTTTGG ATGAAGATTC ACAGCGCCGT TTAACAACAA ACCCATTACG TATTTTAGAT TCTAAAATTG AGTCGACTCA GAAAATTCTG GAAAATGCGC CGAAATTACA TGACTTCTTA AAAGAAGACA GTTTAAGCCA CTTCCAACAA TTACAAGATT ATTTGACTGC TGCAGGCATT AAATTTGTAA TTAACCAAAA ATTGGTACGT GGTCTAGATT ATTACAATAA AACTGTTTTT GAATGGACAA CAACAGCATT AGGTTCGCAA GGTACAGTAT GTGCAGGTGG ACGCTATGAT GGTTTGGTTG GCCAGTTAAA AGGTAAGGCT GATCAGTCTG TTCCAGCGGT CGGTTTTGCA ATGGGGATGG AGCGTTTATT GCTTCTACTT GAGCAAGTCG AACAAGCTGA AATTGTACGT GACTGTGAAG CTTTCTTAGT TGCGGAGCCT GCTTATCAAT CTAAAGCTTT AGTTTTGGCA GAACAATTAC GTGATCAGTT AGAAGCTGCG AATAGTAACA TCCGCATTAA AACTGGTTCA CAAGGCAGCA TGAAGAGTCA GATGAAAAAA GCTGATCAGG CTGGGGCTGT TTACGCCATA ATTTTGGGTG AACGTGAGTG GGAAGCACAG CAGCTTGCTG TAAAAGAATT GGCAACAGCT GAGCAATCAC AAGTTGCATT GGCTGAACTC GTACCATTCT TAATTGAAAA ATTTACAAAA TAA
Details for AcbaC.00063.a.B1.GE38578
HARVESTED ON:
11/19/2014
SEQUENCED ON:
12/10/2014
EXPECTED MW:
49kDa
OBSERVED MW:
47kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Too many to count (100+)
TOTAL EXPRESSION LEVEL:
Low Expression
SOLUBLE EXPRESSION LEVEL
Moderate Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
100
PERCENT COVERAGE:
97
Details for AcbaC.00063.a.B1.PW37682
PURIFICATION DATe:
3/25/2015
CONCENTRATION:
26mg/ml
OBSERVED MW:
46kDa
EXPRESSION LEVEL:
Low Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
7
VIAL VOLUME:
200µl
PERCENT IDENTITY:
100
PERCENT COVERAGE:
97