AcbaC.00057.a

ABC transporter, ATP-binding protein

CENTER ID: AcbaC.00057.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00057.a.B1.GE38577 full length 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.2599
GenBank: AKA32367.1
PGAT: acinetobacter:ABUW_2645
RefSeq: WP_001183458.1
UniProt: V5VEU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQQALQQPQT LCIKHLAKNY SKRWVVKDVS FSMQSGQIVG LLGPNGAGKT TSFYMVVGLV RMDKGEIHLD DLDMSDLAMH ERARKGIGYL PQEASIFRKL TIAENIMSIL ETRKDLNKQQ RQQRLTELLN DFKISHIKDS LGMSVSGGER RRAEIARALA ADPKFMLLDE PFAGVDPISV GDIKDIIQTL KDRGIGVLIT DHNVRETLAI CERAYIVSEG SVIAEGTPQE ILDNEQVRKV YLGDDFTV
NT Sequence
ATGCAGCAAG CTCTTCAACA ACCGCAAACT TTGTGTATTA AACACTTAGC AAAAAACTAT AGTAAGCGTT GGGTTGTCAA AGACGTCTCT TTTAGTATGC AAAGTGGACA GATTGTTGGT TTGCTTGGTC CTAACGGCGC TGGAAAAACC ACCAGTTTCT ATATGGTGGT TGGACTAGTG CGCATGGATA AAGGTGAGAT TCATCTTGAT GACTTGGATA TGTCAGACCT AGCGATGCAT GAACGTGCAC GTAAGGGTAT TGGTTATTTA CCTCAAGAAG CTTCTATCTT TAGAAAATTG ACGATTGCTG AAAATATTAT GTCGATCTTA GAGACGCGTA AAGATTTGAA TAAACAGCAA CGTCAGCAGC GCCTTACTGA GTTATTAAAT GACTTTAAAA TTAGTCATAT TAAAGATTCA TTGGGAATGA GTGTATCTGG TGGTGAACGT CGTCGTGCAG AAATTGCACG TGCTTTGGCC GCAGATCCGA AATTTATGCT TCTGGATGAG CCGTTCGCTG GTGTTGACCC AATTTCAGTA GGCGATATTA AAGATATTAT CCAGACCTTA AAGGATCGCG GTATTGGTGT CCTCATTACC GATCACAATG TTCGTGAAAC TTTAGCAATC TGTGAACGTG CTTATATTGT AAGTGAAGGC TCGGTAATTG CCGAAGGCAC ACCACAAGAA ATTTTAGACA ATGAACAGGT ACGTAAAGTC TATTTAGGTG ATGATTTCAC AGTTTAA
Details for AcbaC.00057.a.B1.GE38577
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 29kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMQ QALQQPQTLC IKHLAKNYSK RWVVKDVSFS MQSGQIVGLL GPNGAGKTTS FYMVVGLVRM DKGEIHLDDL DMSDLAMHER ARKGIGYLPQ EASIFRKLTI AENIMSILET RKDLNKQQRQ QRLTELLNDF KISHIKDSLG MSVSGGERRR AEIARALAAD PKFMLLDEPF AGVDPISVGD IKDIIQTLKD RGIGVLITDH NVRETLAICE RAYIVSEGSV IAEGTPQEIL DNEQVRKVYL GDDFTV
Validated NT Sequence
atcttactca cttaaactgt gaaatcatca cctaaataga ctttacgtac ctgttcattg tctaaaattt cttgtggtgt gccttcggca attaccgagc cttcacttac aatataagca cgttcacaga ttgctaaagt ttcacgaaca ttgtgatcgg taatgaggac accaataccg cgatccttta aggtctggat aatatcttta atatcgccta ctgaaattgg gtcaacacca gcgaacggct catccagaag cataaatttc ggatctgcgg ccaaagcacg tgcaatttct gcacgacgac gttcaccacc agatacactc attcccaatg aatctttaat atgactaatt ttaaagtcat ttaataactc agtaaggcgc tgctgacgtt gctgtttatt caaatcttta cgcgtctcta agatcgacat aatattttca gcaatcgtca attttctaaa gatagaagct tcttgaggta aataaccaat acccttacgt gcacgttcat gcatcgctag gtctgacata tccaagtcat caagatgaat ctcaccttta tccatgcgca ctagtccaac caccatatag aaactggtgg tttttccagc gccgttagga ccaagcaaac caacaatctg tccactttgc atactaaaag agacgtcttt gacaacccaa cgcttactat agttttttgc taagtgttta atacacaaag tttgcggttg ttgaagagct tgctgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMQ QALQQPQTLC IKHLAKNYSK RWVVKDVSFS MQSGQIVGLL GPNGAGKTTS FYMVVGLVRM DKGEIHLDDL DMSDLAMHER ARKGIGYLPQ EASIFRKLTI AENIMSILET RKDLNKQQRQ QRLTELLNDF KISHIKDSLG MSVSGGERRR AEIARALAAD PKFMLLDEPF AGVDPISVGD IKDIIQTLKD RGIGVLITDH NVRETLAICE RAYIVSEGSV IAEGTPQEIL DNEQVRKVYL GDDFTV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagc aagctcttca acaaccgcaa actttgtgta ttaaacactt agcaaaaaac tatagtaagc gttgggttgt caaagacgtc tcttttagta tgcaaagtgg acagattgtt ggtttgcttg gtcctaacgg cgctggaaaa accaccagtt tctatatggt ggttggacta gtgcgcatgg ataaaggtga gattcatctt gatgacttgg atatgtcaga cctagcgatg catgaacgtg cacgtaaggg tattggttat ttacctcaag aagcttctat ctttagaaaa ttgacgattg ctgaaaatat tatgtcgatc ttagagacgc gtaaagattt gaataaacag caacgtcagc agcgccttac tgagttatta aatgacttta aaattagtca tattaaagat tcattgggaa tgagtgtatc tggtggtgaa cgtcgtcgtg cagaaattgc acgtgctttg gccgcagatc cgaaatttat gcttctggat gagccgttcg ctggtgttga cccaatttca gtaggcgata ttaaagatat tatccagacc ttaaaggatc gcggtattgg tgtcctcatt accgatcaca atgttcgtga aactttagca atctgtgaac gtgcttatat tgtaagtgaa ggctcggtaa ttgccgaagg cacaccacaa gaaattttag acaatgaaca ggtacgtaaa gtctatttag gtgatgattt cacagtttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at