AcbaC.00054.a
tRNA (guanine-N1)-methyltransferase
| CENTER ID: | AcbaC.00054.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00054.a.B1.GE43932 | full length | 1 | 246 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00054.a.B1.PW38905 | full length | 1 | 246 |
Structures
7MYQ
DEPOSITED: 5/21/2021
DETERMINATION: XRay
CLONE: AcbaC.00054.a.B1.GE43932
PROTEIN: AcbaC.00054.a.B1.PW38905
7MYS
DEPOSITED: 5/21/2021
DETERMINATION: XRay
CLONE: AcbaC.00054.a.B1.GE43932
PROTEIN: AcbaC.00054.a.B1.PW38905
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30108.1 |
| PATRIC ID: | fig|470.1345.peg.309 |
| PGAT: | acinetobacter:ABUW_0322 |
| RefSeq: | WP_000464595.1 |
| UniProt: | A0A0D5YDX5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
VFFAVITLFP EMFDAITAYG ISGRAAKRDI VQVTCINPRD FAEGNYRRVD ERPFGGGPGM VMMAEPLAKA INHAKQLASR AGCVHVPVVY MSPQGKTLNE QAVQQFVDYD GLIVLCGRYE GVDERLIQHY VDQEWSIGDY VLSGGELPAM VLLDSIIRRL PNVMSDEQSA IQDSFVDGLL DCPQYTKPDQ FEGLDVPEIL KSGHHANIEK WRFLQRYQRT LERRPELIEQ VTLTKQQKKW LSDEQG
NT Sequence
GTGTTTTTTG CAGTCATTAC GCTTTTTCCT GAAATGTTTG ACGCGATTAC AGCCTACGGG ATTAGCGGGC GTGCAGCAAA ACGTGACATA GTACAAGTCA CTTGTATTAA TCCACGTGAT TTTGCTGAAG GGAATTACCG AAGAGTGGAT GAACGTCCAT TTGGTGGTGG CCCTGGTATG GTGATGATGG CGGAGCCTTT GGCAAAAGCA ATTAACCATG CCAAACAACT TGCTTCGCGA GCAGGATGTG TTCATGTACC TGTGGTGTAT ATGTCTCCAC AAGGCAAAAC TTTAAATGAA CAGGCAGTAC AGCAGTTTGT CGATTATGAT GGCTTAATTG TATTGTGTGG GCGTTATGAA GGCGTCGATG AGCGTTTGAT CCAGCATTAT GTTGATCAGG AATGGTCGAT TGGAGATTAC GTCTTATCTG GTGGTGAACT ACCTGCCATG GTGTTGCTTG ATAGTATTAT TCGCCGTTTG CCGAATGTAA TGTCAGATGA GCAATCAGCG ATACAGGATT CTTTTGTAGA TGGTCTTTTG GATTGTCCGC AATACACTAA GCCAGACCAG TTTGAAGGGT TAGATGTTCC TGAAATTTTA AAGTCGGGAC ATCATGCCAA TATTGAGAAA TGGCGTTTTT TGCAGCGTTA TCAACGAACT TTAGAGCGTC GACCTGAGTT GATTGAACAG GTTACGTTGA CTAAGCAGCA AAAAAAATGG CTGTCAGATG AACAAGGGTA A
Details for AcbaC.00054.a.B1.GE43932
| HARVESTED ON: | 12/3/2020 |
| SEQUENCED ON: | 12/16/2020 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV FFAVITLFPE MFDAITAYGI SGRAAKRDIV QVTCINPRDF AEGNYRRVDE RPFGGGPGMV MMAEPLAKAI NHAKQLASRA GCVHVPVVYM SPQGKTLNEQ AVQQFVDYDG LIVLCGRYEG VDERLIQHYV DQEWSIGDYV LSGGELPAMV LLDSIIRRLP NVMSDEQSAI QDSFVDGLLD CPQYTKPDQF EGLDVPEILK SGHHANIEKW RFLQRYQRTL ERRPELIEQV TLTKQQKKWL SDEQG
Validated NT Sequence
atggctcacc accaccacca ccaccaccat atggtgtccg tcccgaccca tcagcaagac ctgatcgccc tgctcgaaga gcgtggcttc gtccaccagt gcaccgaccg cgacggcctc gccgcgcacc tggccgccgg tccggcaacc gcctacctgg gcttcgacgc caccgccgac agcctgcacg tcggccacct acagggcctg atgctgatgc gctggctaca gaaggccggg caccgcccgt tgctgctgat cggtggcgcg accacccgga tcggcgatcc gagcttccgc gattcgagcc ggccgatcct caccgaggcg cagatccagg ccaacatcga cggcatcgcc cgggttttct cgcgctacgt cgaactgcac gacgacagcc tggtgaacaa cgccgaatgg ctggacggcg tcggctacct ggagttcctc gaccgggtcg gccggcactt ctcgatcaac cgcctgctga ccttcgacgc catcaggcag cgcctggacc gcgagcattc gctgtcgttc ctcgagttcg gctacaccct gctgcaggcc tacgatttcg tcgaactgtc gcgccggcgc ggctgcaccc tgcaactcgg cggcgccgac cagtgggcga acatcatcaa cggcgtggag ctgtcgcgac gccagggcgg cgcccagctg ttcggcctga ccatgccgct gctggccacc agtgacgggc gcaagatggg caagtcggcg canggtgcgg tatggctcaa cgccgagcgc ctggcgccgt tcgacttctg gcagttctgg cgcaactgcg atgatcgcga cgtcggtcgc ttcctcgccc tgttcagcga actgccgatg gat
Expected Protein Sequence
MAHHHHHHVF FAVITLFPEM FDAITAYGIS GRAAKRDIVQ VTCINPRDFA EGNYRRVDER PFGGGPGMVM MAEPLAKAIN HAKQLASRAG CVHVPVVYMS PQGKTLNEQA VQQFVDYDGL IVLCGRYEGV DERLIQHYVD QEWSIGDYVL SGGELPAMVL LDSIIRRLPN VMSDEQSAIQ DSFVDGLLDC PQYTKPDQFE GLDVPEILKS GHHANIEKWR FLQRYQRTLE RRPELIEQVT LTKQQKKWLS DEQG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtttt ttgcagtcat tacgcttttt cctgaaatgt ttgacgcgat tacagcctac gggattagcg ggcgtgcagc aaaacgtgac atagtacaag tcacttgtat taatccacgt gattttgctg aagggaatta ccgaagagtg gatgaacgtc catttggtgg tggccctggt atggtgatga tggcggagcc tttggcaaaa gcaattaacc atgccaaaca acttgcttcg cgagcaggat gtgttcatgt acctgtggtg tatatgtctc cacaaggcaa aactttaaat gaacaggcag tacagcagtt tgtcgattat gatggcttaa ttgtattgtg tgggcgttat gaaggcgtcg atgagcgttt gatccagcat tatgttgatc aggaatggtc gattggagat tacgtcttat ctggtggtga actacctgcc atggtgttgc ttgatagtat tattcgccgt ttgccgaatg taatgtcaga tgagcaatca gcgatacagg attcttttgt agatggtctt ttggattgtc cgcaatacac taagccagac cagtttgaag ggttagatgt tcctgaaatt ttaaagtcgg gacatcatgc caatattgag aaatggcgtt ttttgcagcg ttatcaacga actttagagc gtcgacctga gttgattgaa caggttacgt tgactaagca gcaaaaaaaa tggctgtcag atgaacaagg gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for AcbaC.00054.a.B1.PW38905
| PURIFICATION DATe: | 12/11/2020 |
| CONCENTRATION: | 27mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | MAHHHHHHVF FAVITLFPEM FDAITAYGIS GRAAKRDIVQ VTCINPRDFA EGNYRRVDER PFGGGPGMVM MAEPLAKAIN HAKQLASRAG CVHVPVVYMS PQGKTLNEQA VQQFVDYDGL IVLCGRYEGV DERLIQHYVD QEWSIGDYVL SGGELPAMVL LDSIIRRLPN VMSDEQSAIQ DSFVDGLLDC PQYTKPDQFE GLDVPEILKS GHHANIEKWR FLQRYQRTLE RRPELIEQVT LTKQQKKWLS DEQG |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV FFAVITLFPE MFDAITAYGI SGRAAKRDIV QVTCINPRDF AEGNYRRVDE RPFGGGPGMV MMAEPLAKAI NHAKQLASRA GCVHVPVVYM SPQGKTLNEQ AVQQFVDYDG LIVLCGRYEG VDERLIQHYV DQEWSIGDYV LSGGELPAMV LLDSIIRRLP NVMSDEQSAI QDSFVDGLLD CPQYTKPDQF EGLDVPEILK SGHHANIEKW RFLQRYQRTL ERRPELIEQV TLTKQQKKWL SDEQG
Validated NT Sequence
atggctcacc accaccacca ccaccaccat atggtgtccg tcccgaccca tcagcaagac ctgatcgccc tgctcgaaga gcgtggcttc gtccaccagt gcaccgaccg cgacggcctc gccgcgcacc tggccgccgg tccggcaacc gcctacctgg gcttcgacgc caccgccgac agcctgcacg tcggccacct acagggcctg atgctgatgc gctggctaca gaaggccggg caccgcccgt tgctgctgat cggtggcgcg accacccgga tcggcgatcc gagcttccgc gattcgagcc ggccgatcct caccgaggcg cagatccagg ccaacatcga cggcatcgcc cgggttttct cgcgctacgt cgaactgcac gacgacagcc tggtgaacaa cgccgaatgg ctggacggcg tcggctacct ggagttcctc gaccgggtcg gccggcactt ctcgatcaac cgcctgctga ccttcgacgc catcaggcag cgcctggacc gcgagcattc gctgtcgttc ctcgagttcg gctacaccct gctgcaggcc tacgatttcg tcgaactgtc gcgccggcgc ggctgcaccc tgcaactcgg cggcgccgac cagtgggcga acatcatcaa cggcgtggag ctgtcgcgac gccagggcgg cgcccagctg ttcggcctga ccatgccgct gctggccacc agtgacgggc gcaagatggg caagtcggcg canggtgcgg tatggctcaa cgccgagcgc ctggcgccgt tcgacttctg gcagttctgg cgcaactgcg atgatcgcga cgtcggtcgc ttcctcgccc tgttcagcga actgccgatg gat
Expressed Protein Sequence
MAHHHHHHVF FAVITLFPEM FDAITAYGIS GRAAKRDIVQ VTCINPRDFA EGNYRRVDER PFGGGPGMVM MAEPLAKAIN HAKQLASRAG CVHVPVVYMS PQGKTLNEQA VQQFVDYDGL IVLCGRYEGV DERLIQHYVD QEWSIGDYVL SGGELPAMVL LDSIIRRLPN VMSDEQSAIQ DSFVDGLLDC PQYTKPDQFE GLDVPEILKS GHHANIEKWR FLQRYQRTLE RRPELIEQVT LTKQQKKWLS DEQG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTTTT TTGCAGTCAT TACGCTTTTT CCTGAAATGT TTGACGCGAT TACAGCCTAC GGGATTAGCG GGCGTGCAGC AAAACGTGAC ATAGTACAAG TCACTTGTAT TAATCCACGT GATTTTGCTG AAGGGAATTA CCGAAGAGTG GATGAACGTC CATTTGGTGG TGGCCCTGGT ATGGTGATGA TGGCGGAGCC TTTGGCAAAA GCAATTAACC ATGCCAAACA ACTTGCTTCG CGAGCAGGAT GTGTTCATGT ACCTGTGGTG TATATGTCTC CACAAGGCAA AACTTTAAAT GAACAGGCAG TACAGCAGTT TGTCGATTAT GATGGCTTAA TTGTATTGTG TGGGCGTTAT GAAGGCGTCG ATGAGCGTTT GATCCAGCAT TATGTTGATC AGGAATGGTC GATTGGAGAT TACGTCTTAT CTGGTGGTGA ACTACCTGCC ATGGTGTTGC TTGATAGTAT TATTCGCCGT TTGCCGAATG TAATGTCAGA TGAGCAATCA GCGATACAGG ATTCTTTTGT AGATGGTCTT TTGGATTGTC CGCAATACAC TAAGCCAGAC CAGTTTGAAG GGTTAGATGT TCCTGAAATT TTAAAGTCGG GACATCATGC CAATATTGAG AAATGGCGTT TTTTGCAGCG TTATCAACGA ACTTTAGAGC GTCGACCTGA GTTGATTGAA CAGGTTACGT TGACTAAGCA GCAAAAAAAA TGGCTGTCAG ATGAACAAGG GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT