AcbaC.00035.a

phosphoribosylpyrophosphate synthetase

CENTER ID: AcbaC.00035.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00035.a.B1.GE38574 full length 1 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3042
GenBank: AKA32804.1
PGAT: acinetobacter:ABUW_3097
RefSeq: WP_001133287.1
UniProt: A0A0Q1HQY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPNLVVFSGN AHPQFAQKVV SHLHIPLGAA SVGQFSDGEI SVEITENVRG KDVFIVQPTC APTNDNLMEI LVMADALRRA SAGRITAVIP YFGYARQDRR PRSARVPITA KVVADMLTTV GIDRVVMIDL HADQIQGFFD IPVDNIYGTP ALLADLRQQQ HDNLMVVSPD VGGVVRARAV AKQMGDIDLA IIDKRRQKAN ESQVMHLIGD VKDRDCVIVD DMVDTAGTLC KAADALKQFG ARRVVAYATH PVLSGKAIEN LRNSVIDELV VTDTIPLSEE ALNLGKIRQV SVASMVAETI RRINNEESIS AMFDSL
NT Sequence
ATGCCCAATC TTGTCGTTTT TAGTGGAAAT GCTCATCCAC AGTTCGCTCA AAAAGTCGTA AGTCACTTAC ATATCCCTCT AGGTGCTGCA TCAGTCGGTC AGTTCTCTGA TGGTGAAATT TCAGTAGAAA TTACTGAAAA TGTTCGTGGT AAAGACGTAT TTATCGTTCA GCCTACTTGT GCTCCAACCA ATGACAACCT TATGGAAATC TTGGTTATGG CAGATGCTTT GCGTCGTGCA AGTGCTGGTC GTATTACTGC TGTTATCCCT TATTTTGGTT ATGCTCGCCA AGACCGTCGC CCACGTTCTG CACGTGTGCC GATCACAGCG AAAGTTGTTG CAGACATGCT TACCACTGTT GGTATTGACC GTGTCGTAAT GATTGACTTA CATGCTGACC AAATCCAGGG CTTCTTCGAT ATTCCAGTCG ACAACATCTA CGGTACTCCT GCTTTGCTTG CTGACTTACG TCAACAACAA CATGACAACC TTATGGTGGT TTCTCCTGAC GTTGGCGGTG TTGTACGTGC TCGTGCCGTT GCTAAACAGA TGGGTGATAT CGATTTAGCA ATCATCGACA AACGTCGTCA AAAAGCCAAC GAGTCGCAAG TTATGCACTT GATTGGTGAC GTAAAAGATC GTGATTGCGT TATCGTAGAC GACATGGTTG ATACAGCTGG TACATTGTGT AAAGCAGCTG ATGCGCTTAA ACAATTCGGT GCACGTCGTG TTGTTGCTTA CGCAACTCAC CCTGTACTTT CAGGTAAAGC AATTGAGAAC TTACGTAACT CTGTTATTGA TGAGTTAGTT GTAACTGACA CAATTCCTCT TTCTGAAGAA GCATTAAACT TAGGTAAAAT TCGCCAAGTT TCTGTTGCTA GCATGGTTGC TGAAACGATT CGTCGTATTA ATAACGAAGA GTCTATCAGC GCGATGTTCG ATAGTTTATA A
Details for AcbaC.00035.a.B1.GE38574
HARVESTED ON: data unavailable
SEQUENCED ON: 12/10/2014
EXPECTED MW: 35kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP NLVVFSGNAH PQFAQKVVSH LHIPLGAASV GQFSDGEISV EITENVRGKD VFIVQPTCAP TNDNLMEILV MADALRRASA GRITAVIPYF GYARQDRRPR SARVPITAKV VADMLTTVGI DRVVMIDLHA DQIQGFFDIP VDNIYGTPAL LADLRQQQHD NLMVVSPDVG GVVRARAVAK QMGDIDLAII DKRRQKANES QVMHLIGDVK DRDCVIVDDM VDTAGTLCKA ADALKQFGAR RVVAYATHPV LSGKAIENLR NSVIDELVVT DTIPLSEEAL NLGKIRQVSV ASMVAETIRR INNEESIXXM FDSL
Validated NT Sequence
ttactcactt ataaactatc gaacatnnnn nngatagact cttcgttatt aatacgacga atcgtttcag caaccatgct agcaacagaa acttggcgaa ttttacctaa gtttaatgct tcttcagaaa gaggaattgt gtcagttaca actaactcat caataacaga gttacgtaag ttctcaattg ctttacctga aagtacaggg tgagttgcgt aagcaacaac acgacgtgca ccgaattgtt taagcgcatc agctgcttta cacaatgtac cagctgtatc aaccatgtcg tctacgataa cgcaatcacg atcttttacg tcaccaatca agtgcataac ttgcgactcg ttggcttttt gacgacgttt gtcgatgatt gctaaatcga tatcacccat ctgtttagca acggcacgag cacgtacaac accgccaacg tcaggagaaa ccaccataag gttgtcatgt tgttgttgac gtaagtcagc aagcaaagca ggagtaccgt agatgttgtc gactggaata tcgaagaagc cctggatttg gtcagcatgt aagtcaatca ttacgacacg gtcaatacca acagtggtaa gcatgtctgc aacaactttc gctgtgatcg gcacacgtgc agaacgtggg cgacggtctt ggcgagcata accaaaataa gggataacag cagtaatacg accagcactt gcacgacgca aagcatctgc cataaccaag atttccataa ggttgtcatt ggttggagca caagtaggct gaacgataaa tacgtcttta ccacgaacat tttcagtaat ttctactgaa atttcaccat cagagaactg accgactgat gcagcaccta gagggatatg taagtgactt acgacttttt gagcgaactg tggatgagca tttccactaa aaacgacaag attgggcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMP NLVVFSGNAH PQFAQKVVSH LHIPLGAASV GQFSDGEISV EITENVRGKD VFIVQPTCAP TNDNLMEILV MADALRRASA GRITAVIPYF GYARQDRRPR SARVPITAKV VADMLTTVGI DRVVMIDLHA DQIQGFFDIP VDNIYGTPAL LADLRQQQHD NLMVVSPDVG GVVRARAVAK QMGDIDLAII DKRRQKANES QVMHLIGDVK DRDCVIVDDM VDTAGTLCKA ADALKQFGAR RVVAYATHPV LSGKAIENLR NSVIDELVVT DTIPLSEEAL NLGKIRQVSV ASMVAETIRR INNEESISAM FDSL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca atcttgtcgt ttttagtgga aatgctcatc cacagttcgc tcaaaaagtc gtaagtcact tacatatccc tctaggtgct gcatcagtcg gtcagttctc tgatggtgaa atttcagtag aaattactga aaatgttcgt ggtaaagacg tatttatcgt tcagcctact tgtgctccaa ccaatgacaa ccttatggaa atcttggtta tggcagatgc tttgcgtcgt gcaagtgctg gtcgtattac tgctgttatc ccttattttg gttatgctcg ccaagaccgt cgcccacgtt ctgcacgtgt gccgatcaca gcgaaagttg ttgcagacat gcttaccact gttggtattg accgtgtcgt aatgattgac ttacatgctg accaaatcca gggcttcttc gatattccag tcgacaacat ctacggtact cctgctttgc ttgctgactt acgtcaacaa caacatgaca accttatggt ggtttctcct gacgttggcg gtgttgtacg tgctcgtgcc gttgctaaac agatgggtga tatcgattta gcaatcatcg acaaacgtcg tcaaaaagcc aacgagtcgc aagttatgca cttgattggt gacgtaaaag atcgtgattg cgttatcgta gacgacatgg ttgatacagc tggtacattg tgtaaagcag ctgatgcgct taaacaattc ggtgcacgtc gtgttgttgc ttacgcaact caccctgtac tttcaggtaa agcaattgag aacttacgta actctgttat tgatgagtta gttgtaactg acacaattcc tctttctgaa gaagcattaa acttaggtaa aattcgccaa gtttctgttg ctagcatggt tgctgaaacg attcgtcgta ttaataacga agagtctatc agcgcgatgt tcgatagttt atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat