AcbaC.00030.a

ATP-dependent Clp protease, ATP-binding subunit ClpX

CENTER ID: AcbaC.00030.a
ORGANISM: Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaC.00030.a.B1.GE38573 full length 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|470.1345.peg.3336
GenBank: AKA33096.1
PGAT: acinetobacter:ABUW_3400
RefSeq: WP_001289250.1
UniProt: V5VID9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEHPQGQKH CSFCGKTQSE VGKLIAGEDA YICNECVDVC LDLVQTSQQV EAGDWASKAL PKPHEIRAAL DQYVIGQDLA KKTLSVAVYN HYKRLKVGQS GHVSKDVEIA KSNILLIGPT GSGKTLLAQT LARLLDVPFA MADATTLTEA GYVGEDVENI VQKLLQKADY DVEKAQKGII YIDEIDKITR KSENPSITRD VSGEGVQQAL LKMIEGTVAS IPPQGGRKHP QQEFIQIDTS NILFICGGAF AGLEKIVQQR QEKGGIGFTA DVKNKDETKK LAELFRQVEP TDLVKFGLIP EFIGRLPVIA TLEELDEEAL MQILTEPKNA LTRQYQYLFN MENVDLVFED SALRAVAKRA LERNTGARGL RSILENVLLE TMYDLPSRTD VGTVFINEAV INGEAEPVYK SERQPKEAVT HESVAKADLK VIDSKSA
NT Sequence
ATGTCCGAAC ATCCTCAAGG ACAAAAACAT TGTTCATTTT GCGGTAAAAC GCAGTCTGAA GTCGGGAAAC TGATTGCGGG CGAGGACGCA TATATTTGTA ATGAGTGTGT AGATGTCTGC TTAGACCTTG TACAAACCAG CCAACAGGTT GAAGCAGGTG ACTGGGCGAG CAAGGCATTG CCAAAACCAC ATGAAATACG TGCTGCGCTT GATCAATATG TAATTGGTCA GGATCTTGCC AAAAAGACAT TATCTGTTGC CGTTTATAAC CATTATAAGC GTTTGAAAGT CGGGCAATCT GGTCATGTGT CTAAAGATGT GGAAATTGCT AAAAGTAACA TTCTACTGAT TGGGCCTACC GGTTCAGGTA AAACATTACT TGCTCAAACA TTGGCTCGTT TGTTAGATGT GCCGTTTGCA ATGGCAGATG CGACTACTTT GACTGAAGCG GGTTATGTTG GCGAAGACGT TGAAAATATC GTACAAAAGC TTTTGCAAAA AGCAGATTAC GATGTAGAAA AAGCTCAAAA GGGCATTATC TACATTGATG AAATCGACAA GATTACACGT AAATCTGAAA ATCCGTCGAT TACTCGTGAC GTGTCTGGTG AAGGTGTACA ACAAGCTTTG CTTAAGATGA TTGAAGGTAC TGTAGCCTCA ATTCCACCAC AAGGTGGACG TAAGCATCCA CAGCAAGAGT TCATTCAAAT TGATACCTCA AATATTCTAT TTATTTGTGG TGGTGCGTTT GCTGGACTGG AAAAAATTGT ACAACAGCGT CAAGAGAAAG GCGGAATTGG CTTTACAGCT GATGTTAAAA ACAAAGATGA AACCAAAAAA CTTGCTGAAT TATTCCGTCA AGTTGAGCCA ACTGATTTAG TTAAATTTGG TTTAATTCCA GAATTCATTG GCCGTTTACC AGTGATTGCA ACGCTTGAAG AGCTTGATGA AGAAGCATTA ATGCAAATTT TGACTGAACC GAAAAATGCA TTAACTCGTC AGTATCAATA CCTGTTCAAT ATGGAAAATG TTGACCTCGT ATTTGAAGAT TCAGCTTTAC GTGCAGTGGC TAAGCGTGCG CTTGAGCGTA ATACCGGAGC TCGTGGCCTA CGCTCGATTT TGGAAAATGT TCTACTTGAA ACGATGTATG ACTTGCCAAG CCGTACAGAT GTAGGAACAG TTTTCATTAA TGAAGCAGTT ATTAATGGTG AAGCTGAACC TGTCTACAAG TCAGAACGTC AGCCTAAAGA AGCAGTAACA CATGAAAGTG TTGCTAAGGC AGATTTAAAA GTAATTGATT CGAAGTCTGC TTAG
Details for AcbaC.00030.a.B1.GE38573
HARVESTED ON: 11/19/2014
SEQUENCED ON: 12/10/2014
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS EHPQGQKHCS FCGKTQSEVG KLIAGEDAYI CNECVDVCLD LVQTSQQVEA GDWASKALPK PHEIRAALDQ YVIGQDLAKK TLSVAVYNHY KRLKVGQSGH VSKDVEIAKS NILLIGPTGS GKTLLAQTLA RLLDVPFAMA DATTLTEAGY VGEDVENIVQ KLLQKADYDV EKAQKGIIYI DEIDKITRKS ENPSITRDVS GEGVQQALLK MIEGTVASIP PQGGRKHPQQ EFIQIDTSNI LFICGGAFAG LEKIVQQRQE KGGIGFTADV KNKDETKKLA ELFRQVEPTD LVKFGLIPEF IGRLPVIATL EELDEEALMQ ILTEPKNALT RQYQYMFNME NVDLVFEDSA LRAVAKRALE RNTGARGLRS ILENVLLETM YDLPSRTDVG TVFINEAVIN GEAEPVYKSE RQPKEAVTHE SVAKADL
Validated NT Sequence
tttaaatctg ccttagcaac actttcatgt gttactgctt ctttaggctg acgttctgac ttgtagacag gttcagcttc accattaata actgcttcat taatgaaaac tgttcctaca tctgtacggc ttggcaagtc atacatcgtt tcaagtagaa cattttccaa aatcgagcgt aggccacgag ctccggtatt acgctcaagc gcacgcttag ccactgcacg taaagctgaa tcttcaaata cgaggtcaac attttccata ttgaacatgt attgatactg acgagttaat gcatttttcg gttcagtcaa aatttgcatt aatgcttctt catcaagctc ttcaagcgtt gcaatcactg gtaaacggcc aatgaattct ggaattaaac caaatttaac taaatcagtt ggctcaactt gacggaataa ttcagcaagt tttttggttt catctttgtt tttaacatca gctgtaaagc caattccgcc tttctcttga cgctgttgta caattttttc cagtccagca aacgcaccac cacaaataaa tagaatattt gaggtatcaa tttgaatgaa ctcttgctgt ggatgcttac gtccaccttg tggtggaatt gaggctacag taccttcaat catcttaagc aaagcttgtt gtacaccttc accagacacg tcacgagtaa tcgacggatt ttcagattta cgtgtaatct tgtcgatttc atcaatgtag ataatgccct tttgagcttt ttctacatcg taatctgctt tttgcaaaag cttttgtacg atattttcaa cgtcttcgcc aacataaccc gcttcagtca aagtagtcgc atctgccatt gcaaacggca catctaacaa acgagccaat gtttgagcaa gtaatgtttt acctgaaccg gtaggcccaa tcagtagaat gttactttta gcaatttcca catctttaga cacatgacca gattgcccga ctttcaaacg cttataatgg ttataaacgg caacagataa tgtctttttg gcaagatcct gaccaattac atattgatca agcgcagcac gtatttcatg tggttttggc aatgccttgc tcgcccagtc acctgcttca acctgttggc tggtttgtac aaggtctaag cagacatcta cacactcatt acaaatatat gcgtcctcgc ccgcaatcag tttcccgact tcagactgcg ttttaccgca aaatgaacaa tgtttttgtc cttgaggatg ttcggacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS EHPQGQKHCS FCGKTQSEVG KLIAGEDAYI CNECVDVCLD LVQTSQQVEA GDWASKALPK PHEIRAALDQ YVIGQDLAKK TLSVAVYNHY KRLKVGQSGH VSKDVEIAKS NILLIGPTGS GKTLLAQTLA RLLDVPFAMA DATTLTEAGY VGEDVENIVQ KLLQKADYDV EKAQKGIIYI DEIDKITRKS ENPSITRDVS GEGVQQALLK MIEGTVASIP PQGGRKHPQQ EFIQIDTSNI LFICGGAFAG LEKIVQQRQE KGGIGFTADV KNKDETKKLA ELFRQVEPTD LVKFGLIPEF IGRLPVIATL EELDEEALMQ ILTEPKNALT RQYQYLFNME NVDLVFEDSA LRAVAKRALE RNTGARGLRS ILENVLLETM YDLPSRTDVG TVFINEAVIN GEAEPVYKSE RQPKEAVTHE SVAKADLKVI DSKSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aacatcctca aggacaaaaa cattgttcat tttgcggtaa aacgcagtct gaagtcggga aactgattgc gggcgaggac gcatatattt gtaatgagtg tgtagatgtc tgcttagacc ttgtacaaac cagccaacag gttgaagcag gtgactgggc gagcaaggca ttgccaaaac cacatgaaat acgtgctgcg cttgatcaat atgtaattgg tcaggatctt gccaaaaaga cattatctgt tgccgtttat aaccattata agcgtttgaa agtcgggcaa tctggtcatg tgtctaaaga tgtggaaatt gctaaaagta acattctact gattgggcct accggttcag gtaaaacatt acttgctcaa acattggctc gtttgttaga tgtgccgttt gcaatggcag atgcgactac tttgactgaa gcgggttatg ttggcgaaga cgttgaaaat atcgtacaaa agcttttgca aaaagcagat tacgatgtag aaaaagctca aaagggcatt atctacattg atgaaatcga caagattaca cgtaaatctg aaaatccgtc gattactcgt gacgtgtctg gtgaaggtgt acaacaagct ttgcttaaga tgattgaagg tactgtagcc tcaattccac cacaaggtgg acgtaagcat ccacagcaag agttcattca aattgatacc tcaaatattc tatttatttg tggtggtgcg tttgctggac tggaaaaaat tgtacaacag cgtcaagaga aaggcggaat tggctttaca gctgatgtta aaaacaaaga tgaaaccaaa aaacttgctg aattattccg tcaagttgag ccaactgatt tagttaaatt tggtttaatt ccagaattca ttggccgttt accagtgatt gcaacgcttg aagagcttga tgaagaagca ttaatgcaaa ttttgactga accgaaaaat gcattaactc gtcagtatca atacctgttc aatatggaaa atgttgacct cgtatttgaa gattcagctt tacgtgcagt ggctaagcgt gcgcttgagc gtaataccgg agctcgtggc ctacgctcga ttttggaaaa tgttctactt gaaacgatgt atgacttgcc aagccgtaca gatgtaggaa cagttttcat taatgaagca gttattaatg gtgaagctga acctgtctac aagtcagaac gtcagcctaa agaagcagta acacatgaaa gtgttgctaa ggcagattta aaagtaattg attcgaagtc tgcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat