SSGCID: Target Page

AcbaC.00011.a

cell division protein FtsZ

CENTER ID:	AcbaC.00011.a
ORGANISM:	Acinetobacter baumannii AB5075-UW
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaC.00011.a.B1.GE38571	full length		1	391

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1345.peg.147
GenBank:	AKA29946.1
PGAT:	acinetobacter:ABUW_0151
RefSeq:	WP_000135734.1
UniProt:	V5V8B6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASFEFIEDE LNDGNGQARF TVFGVGGGGG NAVQHMVQSD IQGVKFVCAN TDKQALDCMN APFKIQLGEQ STRGLGAGAN PEVGQVAAEE SREIIRQHLE GTDMVFVTAG MGGGTGTGAA PVVAEVAKEM GILTVGVVTT PFNFEGRRRQ KSAERGIEAL EAHVDSLIII PNQRLLSVYG DISMKDAYKK ADDVLLNAVR SIFDLVVNRG HINLDFADLK TAMSTRGYAM MGAGLGRGED RARQAAEQAI RSPLLDNVNI INAKGVLINI TGGDDITLRE TEIITDVVNQ IVDLDEGEIF YGTVFDPDAR DELRVTVIAT GLTRNAADAE PRKRNTVSHT STQSAQSVDE DDVPAINKRQ NAENDVNNAP SSTPRSSPMS IQDYLKNQQR K

NT Sequence

ATGGCCTCAT TTGAATTTAT AGAAGATGAA CTAAACGATG GCAACGGTCA AGCCCGTTTC ACTGTATTTG GTGTAGGTGG CGGTGGCGGT AATGCCGTTC AGCACATGGT ACAGTCTGAT ATTCAGGGTG TTAAATTCGT TTGTGCTAAT ACAGATAAAC AAGCACTTGA CTGTATGAAT GCCCCTTTCA AAATTCAGTT AGGTGAGCAA AGTACACGCG GTCTAGGTGC AGGTGCTAAC CCTGAAGTAG GCCAGGTAGC AGCAGAAGAA AGCCGTGAAA TTATTCGTCA GCACCTTGAA GGGACTGACA TGGTGTTTGT AACCGCTGGT ATGGGTGGTG GTACAGGTAC TGGTGCAGCT CCGGTTGTTG CCGAAGTTGC TAAAGAAATG GGGATTTTGA CTGTTGGTGT TGTAACAACT CCATTTAACT TTGAAGGCCG TCGTCGTCAA AAGTCTGCTG AGCGTGGTAT TGAAGCGCTT GAAGCTCATG TTGATTCATT GATTATTATT CCTAACCAAC GTTTATTAAG TGTTTATGGC GATATTTCAA TGAAAGATGC CTATAAAAAG GCAGATGACG TATTGTTAAA CGCTGTACGC AGTATCTTTG ATCTTGTGGT TAACCGTGGT CACATTAACC TTGACTTCGC GGACTTGAAA ACTGCAATGA GTACTCGTGG TTACGCAATG ATGGGTGCTG GTTTGGGCCG TGGTGAAGAC CGTGCGCGTC AAGCAGCTGA ACAAGCTATC CGTAGCCCAT TGCTTGATAA TGTGAATATT ATTAATGCGA AAGGCGTGTT AATTAACATT ACTGGTGGCG ATGACATCAC ATTACGTGAA ACAGAAATCA TTACTGACGT TGTTAACCAG ATTGTTGACC TCGATGAAGG TGAAATCTTC TACGGTACAG TATTTGACCC AGATGCACGT GATGAATTAC GCGTAACGGT GATTGCAACT GGTTTAACAC GTAATGCCGC TGATGCAGAG CCAAGAAAGC GTAATACAGT TAGCCATACA TCAACTCAAT CTGCACAATC TGTAGATGAA GATGACGTTC CTGCAATTAA TAAACGCCAA AATGCGGAAA ATGACGTGAA TAATGCGCCA AGTTCAACAC CGCGTTCTTC ACCAATGAGT ATTCAAGATT ACTTGAAAAA TCAGCAACGT AAGTAA

Details for AcbaC.00011.a.B1.GE38571

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/10/2014
EXPECTED MW:	43kDa
OBSERVED MW:	13kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMA SFEFIEDELN DGNGQARFTV FGVGGGGGNA VQHMVQSDIQ GVKFVCANTD KQALDCMNAP FKIQLGEQST RGLGAGANPE VGQVAAEESR EIIRQHLEGT DMVFVTAGMG GGTGTGAAPV VAEVAKEMGI LTVGVVTTPF NFEGRRRQKS AERGIEALEA HVDSLIIIPN QRLLSVYGDI SMKDAYKKAD DVLLNAVRSI FDLVVNRGHI NLDFADLKTA MSTRGYAMMG AGLGRGEDRA RQAAEQAIRS PLLDNVNIIN AKGVLINITG GDDITLRETE IITDVVNQIV DLDEGEIFYG TVFDPDARDE LRVTVIATGL TRNAADAEPR KRNTVSHTST QSAQSVDEDD VPAINKRQNA ENDVNNAPSS TPRSSPMSIQ

Validated NT Sequence

atcttgaata ctcattggtg aagaacgcgg tgttgaactt ggcgcattat tcacgtcatt ttccgcattt tggcgtttat taattgcagg aacgtcatct tcatctacag attgtgcaga ttgagttgat gtatggctaa ctgtattacg ctttcttggc tctgcatcag cggcattacg tgttaaacca gttgcaatca ccgttacgcg taattcatca cgtgcatctg ggtcaaatac tgtaccgtag aagatttcac cttcatcgag gtcaacaatc tggttaacaa cgtcagtaat gatttctgtt tcacgtaatg tgatgtcatc gccaccagta atgttaatta acacgccttt cgcattaata atattcacat tatcaagcaa tgggctacgg atagcttgtt cagctgcttg acgcgcacgg tcttcaccac ggcccaaacc agcacccatc attgcgtaac cacgagtact cattgcagtt ttcaagtccg cgaagtcaag gttaatgtga ccacggttaa ccacaagatc aaagatactg cgtacagcgt ttaacaatac gtcatctgcc tttttatagg catctttcat tgaaatatcg ccataaacac ttaataaacg ttggttagga ataataatca atgaatcaac atgagcttca agcgcttcaa taccacgctc agcagacttt tgacgacgac ggccttcaaa gttaaatgga gttgttacaa caccaacagt caaaatcccc atttctttag caacttcggc aacaaccgga gctgcaccag tacctgtacc accacccata ccagcggtta caaacaccat gtcagtccct tcaaggtgct gacgaataat ttcacggctt tcttctgctg ctacctggcc tacttcaggg ttagcacctg cacctagacc gcgtgtactt tgctcaccta actgaatttt gaaaggggca ttcatacagt caagtgcttg tttatctgta ttagcacaaa cgaatttaac accctgaata tcagactgta ccatgtgctg aacggcatta ccgccaccgc cacctacacc aaatacagtg aaacgggctt gaccgttgcc atcgtttagt tcatcttcta taaattcaaa tgaggccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcct catttgaatt tatagaagat gaactaaacg atggcaacgg tcaagcccgt ttcactgtat ttggtgtagg tggcggtggc ggtaatgccg ttcagcacat ggtacagtct gatattcagg gtgttaaatt cgtttgtgct aatacagata aacaagcact tgactgtatg aatgcccctt tcaaaattca gttaggtgag caaagtacac gcggtctagg tgcaggtgct aaccctgaag taggccaggt agcagcagaa gaaagccgtg aaattattcg tcagcacctt gaagggactg acatggtgtt tgtaaccgct ggtatgggtg gtggtacagg tactggtgca gctccggttg ttgccgaagt tgctaaagaa atggggattt tgactgttgg tgttgtaaca actccattta actttgaagg ccgtcgtcgt caaaagtctg ctgagcgtgg tattgaagcg cttgaagctc atgttgattc attgattatt attcctaacc aacgtttatt aagtgtttat ggcgatattt caatgaaaga tgcctataaa aaggcagatg acgtattgtt aaacgctgta cgcagtatct ttgatcttgt ggttaaccgt ggtcacatta accttgactt cgcggacttg aaaactgcaa tgagtactcg tggttacgca atgatgggtg ctggtttggg ccgtggtgaa gaccgtgcgc gtcaagcagc tgaacaagct atccgtagcc cattgcttga taatgtgaat attattaatg cgaaaggcgt gttaattaac attactggtg gcgatgacat cacattacgt gaaacagaaa tcattactga cgttgttaac cagattgttg acctcgatga aggtgaaatc ttctacggta cagtatttga cccagatgca cgtgatgaat tacgcgtaac ggtgattgca actggtttaa cacgtaatgc cgctgatgca gagccaagaa agcgtaatac agttagccat acatcaactc aatctgcaca atctgtagat gaagatgacg ttcctgcaat taataaacgc caaaatgcgg aaaatgacgt gaataatgcg ccaagttcaa caccgcgttc ttcaccaatg agtattcaag attacttgaa aaatcagcaa cgtaagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t