AcbaC.00008.a
glycine hydroxymethyltransferase
| CENTER ID: | AcbaC.00008.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00008.a.B1.GE38569 | full length | 1 | 417 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00008.a.B1.PW37629 | full length | 1 | 417 |
Structures
5VMB
DEPOSITED: 4/27/2017
DETERMINATION: XRay
CLONE: AcbaC.00008.a.B1.GE38569
PROTEIN: AcbaC.00008.a.B1.PW37629
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA31079.1 |
| PATRIC ID: | fig|470.1345.peg.1292 |
| PGAT: | acinetobacter:ABUW_1333 |
| RefSeq: | WP_000457893.1 |
| UniProt: | V5VBJ2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFANISISEF DPELAQAIAS EDERQEAHIE LIASENYCSP AVMEAQGSKL TNKYAEGYPG KRYYGGCEFV DVIEQMAIDR AKELFGADYA NVQPHAGSQA NSAVYLALLN PGDTVLGMSL AHGGHLTHGA KVSFSGKTYN AVQYGLNAET GEIDYEEVER LALEHKPRMI VAGFSAYSRV VDWQRFRDIA DKVGAYLFVD MAHVAGLVAA GVYPNPVQIA DVTTTTTHKT LRGPRSGLIL AKANEEIEKK LQSAVFPGNQ GGPLMHAIAA KAICFKEAMS DDFKAYQQQV VKNAQAMAEV FIARGYDVVS GGTDNHLFLL SLIKQDVTGK DADAWLGAAH ITVNKNSVPN DPRSPFVTSG IRIGTPAVTT RGFGEAEVRE LAGWIADVID SKGDEKVIAD VKAKVEAVCA KFPVYAK
NT Sequence
ATGTTTGCCA ATATTTCCAT TTCTGAATTT GATCCAGAAT TAGCTCAAGC AATTGCTTCT GAAGATGAGC GTCAAGAAGC ACATATCGAG TTAATTGCAT CTGAAAACTA TTGCTCTCCT GCGGTAATGG AAGCGCAAGG ATCAAAACTT ACGAACAAAT ACGCAGAAGG TTATCCTGGC AAACGCTATT ATGGCGGTTG CGAATTTGTA GATGTTATTG AGCAAATGGC GATTGACCGT GCTAAAGAAC TTTTTGGTGC AGATTACGCA AACGTTCAAC CACACGCTGG CTCACAAGCT AACTCTGCTG TTTATTTAGC GCTTCTTAAC CCGGGCGATA CAGTTTTAGG TATGAGCTTG GCTCACGGTG GTCACTTGAC TCACGGTGCA AAAGTAAGCT TCTCTGGTAA AACGTATAAC GCTGTTCAGT ATGGTCTAAA TGCTGAAACT GGTGAGATTG ATTACGAAGA AGTTGAACGT TTAGCATTGG AACACAAGCC ACGTATGATC GTTGCTGGTT TCTCTGCTTA TAGCCGTGTT GTAGATTGGC AACGTTTCCG TGACATCGCG GACAAAGTTG GTGCTTACCT TTTTGTTGAT ATGGCTCACG TTGCTGGTCT TGTTGCTGCT GGTGTATATC CAAACCCAGT TCAAATTGCT GACGTAACAA CAACGACGAC TCACAAAACA CTTCGTGGTC CACGTTCTGG TTTAATCCTT GCGAAAGCAA ATGAAGAAAT CGAGAAAAAA CTTCAATCAG CTGTATTCCC TGGTAACCAA GGCGGTCCAT TAATGCATGC GATTGCTGCT AAAGCAATCT GCTTCAAAGA AGCAATGTCT GACGACTTTA AAGCTTACCA ACAACAAGTT GTGAAAAATG CTCAAGCAAT GGCTGAAGTA TTCATTGCTC GTGGTTATGA TGTTGTTTCT GGTGGTACAG ACAACCACTT ATTCTTGTTA TCTTTAATCA AACAAGACGT AACTGGTAAA GATGCAGACG CTTGGTTAGG TGCTGCTCAC ATTACTGTGA ACAAAAACTC AGTTCCAAAT GACCCACGTT CTCCATTTGT GACTTCTGGT ATCCGTATCG GTACTCCGGC AGTAACAACT CGTGGTTTTG GTGAAGCTGA AGTTCGTGAA CTTGCTGGTT GGATCGCTGA CGTGATTGAC AGCAAAGGTG ACGAAAAAGT TATTGCTGAT GTAAAAGCAA AAGTTGAAGC TGTTTGTGCA AAATTCCCTG TATATGCAAA ATAA
Details for AcbaC.00008.a.B1.GE38569
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMF ANISISEFDP ELAQAIASED ERQEAHIELI ASENYCSPAV MEAQGSKLTN KYAEGYPGKR YYGGCEFVDV IEQMAIDRAK ELFGADYANV QPHAGSQANS AVYLALLNPG DTVLGMSLAH GGHLTHGAKV SFSGKTYNAV QYGLNAETGE IDYEEVERLA LEHKPRMIVA GFSAYSRVVD WQRFRDIADK VGAYLFVDMA HVAGLVAAGV YPNPVQIADV TTTTTHKTLR GPRSGLILAK ANEEIEKKLQ SAVFPGNQGG PLMHAIAAKA ICFKEAMSDD FKAYQQQVVK NAQAMAEVFI ARGYDVVSGG TDNHLFLLSL IKQDVTGKDA DAWLGAAHIT VNKNSVPNDP RSPFVTSGIR IGTPAVTTRG FGEAEVRELA GWIADVIDSK GDEKVIADVK AKVEAVC
Validated NT Sequence
tgcacaaaca gcttcaactt ttgcttttac atcagcaata actttttcgt cacctttgct gtcaatcacg tcagcgatcc aaccagcaag ttcacgaact tcagcttcac caaaaccacg agttgttact gccggagtac cgatacggat accagaagtc acaaatggag aacgtgggtc atttggaact gagtttttgt tcacagtaat gtgagcagca cctaaccaag cgtctgcatc tttaccagtt acgtcttgtt tgattaaaga taacaagaat aagtggttgt ctgtaccacc agaaacaaca tcataaccac gagcaatgaa tacttcagcc attgcttgag catttttcac aacttgttgt tggtaagctt taaagtcgtc agacattgct tctttgaagc agattgcttt agcagcaatc gcatgcatta atggaccgcc ttggttacca gggaatacag ctgattgaag ttttttctcg atttcttcat ttgctttcgc aaggattaaa ccagaacgtg gaccacgaag tgttttgtga gtcgtcgttg ttgttacgtc agcaatttga actgggtttg gatatacacc agcagcaaca agaccagcaa cgtgagccat atcaacaaaa aggtaagcac caactttgtc cgcgatgtca cggaaacgtt gccaatctac aacacggcta taagcagaga aaccagcaac gatcatacgt ggcttgtgtt ccaatgctaa acgttcaact tcttcgtaat caatctcacc agtttcagca tttagaccat actgaacagc gttatacgtt ttaccagaga agcttacttt tgcaccgtga gtcaagtgac caccgtgagc caagctcata cctaaaactg tatcgcccgg gttaagaagc gctaaataaa cagcagagtt agcttgtgag ccagcgtgtg gttgaacgtt tgcgtaatct gcaccaaaaa gttctttagc acggtcaatc gccatttgct caataacatc tacaaattcg caaccgccat aatagcgttt gccaggataa ccttctgcgt atttgttcgt aagttttgat ccttgcgctt ccattaccgc aggagagcaa tagttttcag atgcaattaa ctcgatatgt gcttcttgac gctcatcttc agaagcaatt gcttgagcta attctggatc aaattcagaa atggaaatat tggcaaacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMF ANISISEFDP ELAQAIASED ERQEAHIELI ASENYCSPAV MEAQGSKLTN KYAEGYPGKR YYGGCEFVDV IEQMAIDRAK ELFGADYANV QPHAGSQANS AVYLALLNPG DTVLGMSLAH GGHLTHGAKV SFSGKTYNAV QYGLNAETGE IDYEEVERLA LEHKPRMIVA GFSAYSRVVD WQRFRDIADK VGAYLFVDMA HVAGLVAAGV YPNPVQIADV TTTTTHKTLR GPRSGLILAK ANEEIEKKLQ SAVFPGNQGG PLMHAIAAKA ICFKEAMSDD FKAYQQQVVK NAQAMAEVFI ARGYDVVSGG TDNHLFLLSL IKQDVTGKDA DAWLGAAHIT VNKNSVPNDP RSPFVTSGIR IGTPAVTTRG FGEAEVRELA GWIADVIDSK GDEKVIADVK AKVEAVCAKF PVYAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg ccaatatttc catttctgaa tttgatccag aattagctca agcaattgct tctgaagatg agcgtcaaga agcacatatc gagttaattg catctgaaaa ctattgctct cctgcggtaa tggaagcgca aggatcaaaa cttacgaaca aatacgcaga aggttatcct ggcaaacgct attatggcgg ttgcgaattt gtagatgtta ttgagcaaat ggcgattgac cgtgctaaag aactttttgg tgcagattac gcaaacgttc aaccacacgc tggctcacaa gctaactctg ctgtttattt agcgcttctt aacccgggcg atacagtttt aggtatgagc ttggctcacg gtggtcactt gactcacggt gcaaaagtaa gcttctctgg taaaacgtat aacgctgttc agtatggtct aaatgctgaa actggtgaga ttgattacga agaagttgaa cgtttagcat tggaacacaa gccacgtatg atcgttgctg gtttctctgc ttatagccgt gttgtagatt ggcaacgttt ccgtgacatc gcggacaaag ttggtgctta cctttttgtt gatatggctc acgttgctgg tcttgttgct gctggtgtat atccaaaccc agttcaaatt gctgacgtaa caacaacgac gactcacaaa acacttcgtg gtccacgttc tggtttaatc cttgcgaaag caaatgaaga aatcgagaaa aaacttcaat cagctgtatt ccctggtaac caaggcggtc cattaatgca tgcgattgct gctaaagcaa tctgcttcaa agaagcaatg tctgacgact ttaaagctta ccaacaacaa gttgtgaaaa atgctcaagc aatggctgaa gtattcattg ctcgtggtta tgatgttgtt tctggtggta cagacaacca cttattcttg ttatctttaa tcaaacaaga cgtaactggt aaagatgcag acgcttggtt aggtgctgct cacattactg tgaacaaaaa ctcagttcca aatgacccac gttctccatt tgtgacttct ggtatccgta tcggtactcc ggcagtaaca actcgtggtt ttggtgaagc tgaagttcgt gaacttgctg gttggatcgc tgacgtgatt gacagcaaag gtgacgaaaa agttattgct gatgtaaaag caaaagttga agctgtttgt gcaaaattcc ctgtatatgc aaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for AcbaC.00008.a.B1.PW37629
| PURIFICATION DATe: | 1/12/2015 |
| CONCENTRATION: | 37mg/ml |
| OBSERVED MW: | 48kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF ANISISEFDP ELAQAIASED ERQEAHIELI ASENYCSPAV MEAQGSKLTN KYAEGYPGKR YYGGCEFVDV IEQMAIDRAK ELFGADYANV QPHAGSQANS AVYLALLNPG DTVLGMSLAH GGHLTHGAKV SFSGKTYNAV QYGLNAETGE IDYEEVERLA LEHKPRMIVA GFSAYSRVVD WQRFRDIADK VGAYLFVDMA HVAGLVAAGV YPNPVQIADV TTTTTHKTLR GPRSGLILAK ANEEIEKKLQ SAVFPGNQGG PLMHAIAAKA ICFKEAMSDD FKAYQQQVVK NAQAMAEVFI ARGYDVVSGG TDNHLFLLSL IKQDVTGKDA DAWLGAAHIT VNKNSVPNDP RSPFVTSGIR IGTPAVTTRG FGEAEVRELA GWIADVIDSK GDEKVIADVK AKVEAVC
Validated NT Sequence
tgcacaaaca gcttcaactt ttgcttttac atcagcaata actttttcgt cacctttgct gtcaatcacg tcagcgatcc aaccagcaag ttcacgaact tcagcttcac caaaaccacg agttgttact gccggagtac cgatacggat accagaagtc acaaatggag aacgtgggtc atttggaact gagtttttgt tcacagtaat gtgagcagca cctaaccaag cgtctgcatc tttaccagtt acgtcttgtt tgattaaaga taacaagaat aagtggttgt ctgtaccacc agaaacaaca tcataaccac gagcaatgaa tacttcagcc attgcttgag catttttcac aacttgttgt tggtaagctt taaagtcgtc agacattgct tctttgaagc agattgcttt agcagcaatc gcatgcatta atggaccgcc ttggttacca gggaatacag ctgattgaag ttttttctcg atttcttcat ttgctttcgc aaggattaaa ccagaacgtg gaccacgaag tgttttgtga gtcgtcgttg ttgttacgtc agcaatttga actgggtttg gatatacacc agcagcaaca agaccagcaa cgtgagccat atcaacaaaa aggtaagcac caactttgtc cgcgatgtca cggaaacgtt gccaatctac aacacggcta taagcagaga aaccagcaac gatcatacgt ggcttgtgtt ccaatgctaa acgttcaact tcttcgtaat caatctcacc agtttcagca tttagaccat actgaacagc gttatacgtt ttaccagaga agcttacttt tgcaccgtga gtcaagtgac caccgtgagc caagctcata cctaaaactg tatcgcccgg gttaagaagc gctaaataaa cagcagagtt agcttgtgag ccagcgtgtg gttgaacgtt tgcgtaatct gcaccaaaaa gttctttagc acggtcaatc gccatttgct caataacatc tacaaattcg caaccgccat aatagcgttt gccaggataa ccttctgcgt atttgttcgt aagttttgat ccttgcgctt ccattaccgc aggagagcaa tagttttcag atgcaattaa ctcgatatgt gcttcttgac gctcatcttc agaagcaatt gcttgagcta attctggatc aaattcagaa atggaaatat tggcaaacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMF ANISISEFDP ELAQAIASED ERQEAHIELI ASENYCSPAV MEAQGSKLTN KYAEGYPGKR YYGGCEFVDV IEQMAIDRAK ELFGADYANV QPHAGSQANS AVYLALLNPG DTVLGMSLAH GGHLTHGAKV SFSGKTYNAV QYGLNAETGE IDYEEVERLA LEHKPRMIVA GFSAYSRVVD WQRFRDIADK VGAYLFVDMA HVAGLVAAGV YPNPVQIADV TTTTTHKTLR GPRSGLILAK ANEEIEKKLQ SAVFPGNQGG PLMHAIAAKA ICFKEAMSDD FKAYQQQVVK NAQAMAEVFI ARGYDVVSGG TDNHLFLLSL IKQDVTGKDA DAWLGAAHIT VNKNSVPNDP RSPFVTSGIR IGTPAVTTRG FGEAEVRELA GWIADVIDSK GDEKVIADVK AKVEAVCAKF PVYAK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTG CCAATATTTC CATTTCTGAA TTTGATCCAG AATTAGCTCA AGCAATTGCT TCTGAAGATG AGCGTCAAGA AGCACATATC GAGTTAATTG CATCTGAAAA CTATTGCTCT CCTGCGGTAA TGGAAGCGCA AGGATCAAAA CTTACGAACA AATACGCAGA AGGTTATCCT GGCAAACGCT ATTATGGCGG TTGCGAATTT GTAGATGTTA TTGAGCAAAT GGCGATTGAC CGTGCTAAAG AACTTTTTGG TGCAGATTAC GCAAACGTTC AACCACACGC TGGCTCACAA GCTAACTCTG CTGTTTATTT AGCGCTTCTT AACCCGGGCG ATACAGTTTT AGGTATGAGC TTGGCTCACG GTGGTCACTT GACTCACGGT GCAAAAGTAA GCTTCTCTGG TAAAACGTAT AACGCTGTTC AGTATGGTCT AAATGCTGAA ACTGGTGAGA TTGATTACGA AGAAGTTGAA CGTTTAGCAT TGGAACACAA GCCACGTATG ATCGTTGCTG GTTTCTCTGC TTATAGCCGT GTTGTAGATT GGCAACGTTT CCGTGACATC GCGGACAAAG TTGGTGCTTA CCTTTTTGTT GATATGGCTC ACGTTGCTGG TCTTGTTGCT GCTGGTGTAT ATCCAAACCC AGTTCAAATT GCTGACGTAA CAACAACGAC GACTCACAAA ACACTTCGTG GTCCACGTTC TGGTTTAATC CTTGCGAAAG CAAATGAAGA AATCGAGAAA AAACTTCAAT CAGCTGTATT CCCTGGTAAC CAAGGCGGTC CATTAATGCA TGCGATTGCT GCTAAAGCAA TCTGCTTCAA AGAAGCAATG TCTGACGACT TTAAAGCTTA CCAACAACAA GTTGTGAAAA ATGCTCAAGC AATGGCTGAA GTATTCATTG CTCGTGGTTA TGATGTTGTT TCTGGTGGTA CAGACAACCA CTTATTCTTG TTATCTTTAA TCAAACAAGA CGTAACTGGT AAAGATGCAG ACGCTTGGTT AGGTGCTGCT CACATTACTG TGAACAAAAA CTCAGTTCCA AATGACCCAC GTTCTCCATT TGTGACTTCT GGTATCCGTA TCGGTACTCC GGCAGTAACA ACTCGTGGTT TTGGTGAAGC TGAAGTTCGT GAACTTGCTG GTTGGATCGC TGACGTGATT GACAGCAAAG GTGACGAAAA AGTTATTGCT GATGTAAAAG CAAAAGTTGA AGCTGTTTGT GCAAAATTCC CTGTATATGC AAAATGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT