AcbaC.00002.a
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
| CENTER ID: | AcbaC.00002.a |
| ORGANISM: | Acinetobacter baumannii AB5075-UW |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00002.a.B1.GE38567 | full length | 1 | 273 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaC.00002.a.B1.PS02398 | full length | 1 | 273 |
Structures
6AMY
DEPOSITED: 8/11/2017
DETERMINATION: XRay
CLONE: AcbaC.00002.a.B1.GE38567
PROTEIN: AcbaC.00002.a.B1.PS02398
6AMZ
DEPOSITED: 8/11/2017
DETERMINATION: XRay
CLONE: AcbaC.00002.a.B1.GE38567
PROTEIN: AcbaC.00002.a.B1.PS02398
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | AKA30768.1 |
| PATRIC ID: | fig|470.1345.peg.979 |
| PGAT: | acinetobacter:ABUW_1014 |
| RefSeq: | WP_000080867.1 |
| UniProt: | V5VB11 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQLSTIIEQ AFEDRANFTA ADCPSEIRQA VEEAIAGLDN GTLRVAEKIN GEWVVHQWLK KAVLLSFKLN DNKPIESCDL RFYDKVETKF SGWTEEQFKA AGVRVVPPAV ARRGSFQAKN VVLMPSYVNI GAYVDEGTMV DTWATVGSCA QIGKNVHLSG GVGIGGVLEP LQANPTIIED NCFIGARSEI VEGVIVEEGS VISMGVYIGQ STRIYDRETG EIHYGRVPAG SVVVPGNLPS ADGKYSLYAA IIVKKVDAQT RAKTSLNDLL RAD
NT Sequence
ATGTCTCAGC TTTCTACGAT CATTGAGCAA GCATTTGAAG ACCGCGCAAA TTTCACTGCA GCAGATTGCC CAAGCGAAAT TCGCCAAGCT GTTGAAGAAG CAATTGCTGG TTTAGATAAC GGTACACTTC GAGTTGCAGA AAAAATCAAT GGTGAATGGG TTGTTCATCA ATGGTTAAAA AAAGCTGTTT TATTATCATT TAAACTTAAT GACAATAAAC CAATCGAGTC ATGTGATCTT CGTTTCTACG ACAAAGTTGA AACTAAATTC TCTGGTTGGA CTGAAGAGCA ATTTAAAGCT GCGGGTGTTC GTGTTGTTCC TCCTGCTGTT GCTCGTCGTG GTAGTTTCCA AGCTAAAAAT GTGGTGTTAA TGCCATCTTA TGTCAACATT GGTGCTTACG TAGATGAAGG CACAATGGTT GATACATGGG CAACTGTAGG TTCATGTGCT CAAATCGGTA AAAACGTTCA CTTATCAGGT GGTGTTGGTA TCGGTGGTGT TTTAGAACCA TTACAAGCTA ACCCAACCAT TATTGAAGAC AACTGTTTTA TCGGCGCACG TTCTGAAATC GTTGAAGGTG TTATTGTCGA AGAAGGCTCT GTTATTTCTA TGGGTGTTTA TATCGGTCAA TCTACACGTA TTTATGATCG TGAGACTGGT GAAATCCATT ATGGCCGTGT TCCTGCTGGC TCAGTTGTTG TACCAGGTAA CTTACCGTCA GCTGATGGTA AATACAGCTT ATACGCTGCA ATTATCGTGA AAAAAGTTGA TGCACAAACA CGTGCAAAAA CAAGTTTGAA CGATTTGTTA CGCGCTGACT AA
Details for AcbaC.00002.a.B1.GE38567
| HARVESTED ON: | 11/19/2014 |
| SEQUENCED ON: | 12/10/2014 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS QLSTIIEQAF EDRANFTAAD CPSEIRQAVE EAIAGLDNGT LRVAEKINGE WVVHQWLKKA VLLSFKLNDN KPIESCDLRF YDKVETKFSG WTEEQFKAAG VRVVPPAVAR RGSFQAKNVV LMPSYVNIGA YVDEGTMVDT WATVGSCAQI GKNVHLSGGV GIGGVLEPLQ ANPTIIEDNC FIGARSEIVE GVIVEEGSVI SMGVYIGQST RIYDRETGEI HYGRVPAGSV VVPGNLPSAD GKYSLYAAII VKKVDAQTRA KTSLNDLLRA D
Validated NT Sequence
atcttactca cttagtcagc gcgtaacaaa tcgttcaaac ttgtttttgc acgtgtttgt gcatcaactt ttttcacgat aattgcagcg tataagctgt atttaccatc agctgacggt aagttacctg gtacaacaac tgagccagca ggaacacggc cataatggat ttcaccagtc tcacgatcat aaatacgtgt agattgaccg atataaacac ccatagaaat aacagagcct tcttcgacaa taacaccttc aacgatttca gaacgtgcgc cgataaaaca gttgtcttca ataatggttg ggttagcttg taatggttct aaaacaccac cgataccaac accacctgat aagtgaacgt ttttaccgat ttgagcacat gaacctacag ttgcccatgt atcaaccatt gtgccttcat ctacgtaagc accaatgttg acataagatg gcattaacac cacattttta gcttggaaac taccacgacg agcaacagca ggaggaacaa cacgaacacc cgcagcttta aattgctctt cagtccaacc agagaattta gtttcaactt tgtcgtagaa acgaagatca catgactcga ttggtttatt gtcattaagt ttaaatgata ataaaacagc tttttttaac cattgatgaa caacccattc accattgatt ttttctgcaa ctcgaagtgt accgttatct aaaccagcaa ttgcttcttc aacagcttgg cgaatttcgc ttgggcaatc tgctgcagtg aaatttgcgc ggtcttcaaa tgcttgctca atgatcgtag aaagctgaga catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMS QLSTIIEQAF EDRANFTAAD CPSEIRQAVE EAIAGLDNGT LRVAEKINGE WVVHQWLKKA VLLSFKLNDN KPIESCDLRF YDKVETKFSG WTEEQFKAAG VRVVPPAVAR RGSFQAKNVV LMPSYVNIGA YVDEGTMVDT WATVGSCAQI GKNVHLSGGV GIGGVLEPLQ ANPTIIEDNC FIGARSEIVE GVIVEEGSVI SMGVYIGQST RIYDRETGEI HYGRVPAGSV VVPGNLPSAD GKYSLYAAII VKKVDAQTRA KTSLNDLLRA D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtctc agctttctac gatcattgag caagcatttg aagaccgcgc aaatttcact gcagcagatt gcccaagcga aattcgccaa gctgttgaag aagcaattgc tggtttagat aacggtacac ttcgagttgc agaaaaaatc aatggtgaat gggttgttca tcaatggtta aaaaaagctg ttttattatc atttaaactt aatgacaata aaccaatcga gtcatgtgat cttcgtttct acgacaaagt tgaaactaaa ttctctggtt ggactgaaga gcaatttaaa gctgcgggtg ttcgtgttgt tcctcctgct gttgctcgtc gtggtagttt ccaagctaaa aatgtggtgt taatgccatc ttatgtcaac attggtgctt acgtagatga aggcacaatg gttgatacat gggcaactgt aggttcatgt gctcaaatcg gtaaaaacgt tcacttatca ggtggtgttg gtatcggtgg tgttttagaa ccattacaag ctaacccaac cattattgaa gacaactgtt ttatcggcgc acgttctgaa atcgttgaag gtgttattgt cgaagaaggc tctgttattt ctatgggtgt ttatatcggt caatctacac gtatttatga tcgtgagact ggtgaaatcc attatggccg tgttcctgct ggctcagttg ttgtaccagg taacttaccg tcagctgatg gtaaatacag cttatacgct gcaattatcg tgaaaaaagt tgatgcacaa acacgtgcaa aaacaagttt gaacgatttg ttacgcgctg actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for AcbaC.00002.a.B1.PS02398
| PURIFICATION DATe: | 5/8/2015 |
| CONCENTRATION: | 46.12mg/ml |
| OBSERVED MW: | 29kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QLSTIIEQAF EDRANFTAAD CPSEIRQAVE EAIAGLDNGT LRVAEKINGE WVVHQWLKKA VLLSFKLNDN KPIESCDLRF YDKVETKFSG WTEEQFKAAG VRVVPPAVAR RGSFQAKNVV LMPSYVNIGA YVDEGTMVDT WATVGSCAQI GKNVHLSGGV GIGGVLEPLQ ANPTIIEDNC FIGARSEIVE GVIVEEGSVI SMGVYIGQST RIYDRETGEI HYGRVPAGSV VVPGNLPSAD GKYSLYAAII VKKVDAQTRA KTSLNDLLRA D
Validated NT Sequence
atcttactca cttagtcagc gcgtaacaaa tcgttcaaac ttgtttttgc acgtgtttgt gcatcaactt ttttcacgat aattgcagcg tataagctgt atttaccatc agctgacggt aagttacctg gtacaacaac tgagccagca ggaacacggc cataatggat ttcaccagtc tcacgatcat aaatacgtgt agattgaccg atataaacac ccatagaaat aacagagcct tcttcgacaa taacaccttc aacgatttca gaacgtgcgc cgataaaaca gttgtcttca ataatggttg ggttagcttg taatggttct aaaacaccac cgataccaac accacctgat aagtgaacgt ttttaccgat ttgagcacat gaacctacag ttgcccatgt atcaaccatt gtgccttcat ctacgtaagc accaatgttg acataagatg gcattaacac cacattttta gcttggaaac taccacgacg agcaacagca ggaggaacaa cacgaacacc cgcagcttta aattgctctt cagtccaacc agagaattta gtttcaactt tgtcgtagaa acgaagatca catgactcga ttggtttatt gtcattaagt ttaaatgata ataaaacagc tttttttaac cattgatgaa caacccattc accattgatt ttttctgcaa ctcgaagtgt accgttatct aaaccagcaa ttgcttcttc aacagcttgg cgaatttcgc ttgggcaatc tgctgcagtg aaatttgcgc ggtcttcaaa tgcttgctca atgatcgtag aaagctgaga catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMS QLSTIIEQAF EDRANFTAAD CPSEIRQAVE EAIAGLDNGT LRVAEKINGE WVVHQWLKKA VLLSFKLNDN KPIESCDLRF YDKVETKFSG WTEEQFKAAG VRVVPPAVAR RGSFQAKNVV LMPSYVNIGA YVDEGTMVDT WATVGSCAQI GKNVHLSGGV GIGGVLEPLQ ANPTIIEDNC FIGARSEIVE GVIVEEGSVI SMGVYIGQST RIYDRETGEI HYGRVPAGSV VVPGNLPSAD GKYSLYAAII VKKVDAQTRA KTSLNDLLRA D
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCTC AGCTTTCTAC GATCATTGAG CAAGCATTTG AAGACCGCGC AAATTTCACT GCAGCAGATT GCCCAAGCGA AATTCGCCAA GCTGTTGAAG AAGCAATTGC TGGTTTAGAT AACGGTACAC TTCGAGTTGC AGAAAAAATC AATGGTGAAT GGGTTGTTCA TCAATGGTTA AAAAAAGCTG TTTTATTATC ATTTAAACTT AATGACAATA AACCAATCGA GTCATGTGAT CTTCGTTTCT ACGACAAAGT TGAAACTAAA TTCTCTGGTT GGACTGAAGA GCAATTTAAA GCTGCGGGTG TTCGTGTTGT TCCTCCTGCT GTTGCTCGTC GTGGTAGTTT CCAAGCTAAA AATGTGGTGT TAATGCCATC TTATGTCAAC ATTGGTGCTT ACGTAGATGA AGGCACAATG GTTGATACAT GGGCAACTGT AGGTTCATGT GCTCAAATCG GTAAAAACGT TCACTTATCA GGTGGTGTTG GTATCGGTGG TGTTTTAGAA CCATTACAAG CTAACCCAAC CATTATTGAA GACAACTGTT TTATCGGCGC ACGTTCTGAA ATCGTTGAAG GTGTTATTGT CGAAGAAGGC TCTGTTATTT CTATGGGTGT TTATATCGGT CAATCTACAC GTATTTATGA TCGTGAGACT GGTGAAATCC ATTATGGCCG TGTTCCTGCT GGCTCAGTTG TTGTACCAGG TAACTTACCG TCAGCTGATG GTAAATACAG CTTATACGCT GCAATTATCG TGAAAAAAGT TGATGCACAA ACACGTGCAA AAACAAGTTT GAACGATTTG TTACGCGCTG ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT