SSGCID: Target Page

AcbaB.19551.a

hypothetical protein

CENTER ID:	AcbaB.19551.a
ORGANISM:	Acinetobacter baumannii ATCC 17978 / NCDC KC 755
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaB.19551.a.B2.GE41701	mature protein		32	569

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaB.19551.a.B2.PW38401	mature protein		32	569

External Resources

RESOURCE	REFERENCE ID
GenBank:	ABO11270.1
PGAT:	acinetobacter:A1S_0836

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSRINFNGA SIRQYSTTFL LLGSMSSYVY ARPVQETYAV HSFDQALQQS MVAEFALAYD DIPTALHNYT VLAIKSNSTT IKQRALDVAL EYNDLQAALN IATHWVVQEP KDVPALFYLS HIALKTHEYQ LAAETLDKIL KIDPTADLEQ ILASIAPENA QDREVLLTAL RSSAEKDNPS ILALIANLEA QNGQLEPALS TINRALRRRP KVTSFILMKA NLLIALSDQE GALKWYAKSS RKYKKNLDIR LAEVRYLIQI NQPQLALEKL EKIIESNPHA EEALFIAGLT SIDLKQYDKA EQYLVDLRNS AKYQNEAYYY LAINAERKQH YETAKAYYRL VDGSLYIVSR RNLIAIYDKQ ENLHDALRFL TQERVNYPQH ASFLYQAQAE ILKKMGNKKA ALNLLDEAIK NLPDDPELIY AEVLLLDPYT DRDKLDKTLK QLLQLEPNSP TYLNAYAYTL ALQNRRLKEA RQYAEQALEY APEQASILDT LGYIAFLQND YEAAAEALGK AYELSHNINI GIRYAKALYM QGSLTQFSAV LQQLKQKHAN DPQLQQLDAL ILPTSAKKS

NT Sequence

atgaatagcc gtattaattt taacggtgct tcgataagac agtattctac cacattctta ttgttaggta gcatgtcctc atatgtctat gcacgtccag tgcaagagac ctatgccgtg cattcatttg atcaggcttt acagcagagt atggttgctg aatttgcctt agcttatgac gatattccca cagcactgca caattacaca gtgcttgcga taaaaagcaa ctcaacaaca attaaacagc gtgcattaga cgttgcactt gaatataacg acttacaagc tgccctaaat attgcaacac attgggttgt tcaagagcct aaagatgtcc ctgctctatt ttatttatcg catattgcac taaaaacaca cgaatatcag cttgctgccg agactttaga taaaatttta aaaatagatc caactgccga tcttgaacaa attttggcaa gcattgcacc tgaaaatgca caggatcgag aagttttatt aaccgcttta cgttctagtg cagagaaaga caacccatcc atcttagcgc ttattgccaa cctagaagca caaaatggtc agttagaacc agccttaagt accattaatc gcgcactacg ccgtcgccct aaagtgacca gctttatctt aatgaaagcc aatttactaa tcgcactcag tgatcaggaa ggtgctttaa aatggtatgc aaaatcaagc cgtaaatata agaaaaacct agatattcgc ctagccgaag tacgttacct catccagatt aatcaacccc agcttgctct tgaaaaatta gagaaaatta ttgaaagcaa tcctcatgct gaagaggctt tatttattgc tggtttaacc agtattgacc ttaagcaata tgacaaagca gaacaatatc tggttgattt acgcaactct gcaaaatatc aaaatgaggc gtattactac ttagcgatta atgctgaaag aaaacaacat tatgaaactg ctaaagctta ctaccgttta gtcgatggta gtttgtatat tgtttcaaga cgcaatctca ttgcgatcta tgataaacaa gaaaacttac acgacgcttt acgcttttta acccaagagc gagtcaatta tcctcagcat gcaagctttc tttaccaagc tcaagctgaa attttaaaga agatgggcaa taaaaaagct gcattaaatt tattagatga agcgatcaaa aacttaccag atgatccgga gctcatctat gcagaagttt tattattaga cccttatact gaccgcgata aactcgataa aacactcaaa caattattgc agttagaacc caatagccca acgtatctaa acgcctatgc ttatacatta gctttgcaaa atcgtaggct taaggaagca cgtcaatatg ctgaacaggc tttagaatat gccccagaac aagcttctat tctggatacg ctaggctata tcgcattttt acaaaatgat tatgaagcgg ctgcggaagc tttaggcaaa gcatatgaac taagtcataa cataaatatt ggcattcgtt acgctaaggc actttatatg caaggatcat taactcagtt cagtgccgtg ttacaacaac tgaaacaaaa gcacgcaaat gatccacaat tacaacaact tgatgcgtta attttaccta catctgcaaa aaagagttaa

Details for AcbaB.19551.a.B2.GE41701

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	62kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	39

Validated AA Sequence

MVAEFALAYD DIPTALHNYT VLAIKSNSTT IKQRALDVAL EYNDLQAALN IATHWVVQEP KDVPALFYLS HIALKTHEYQ LAAETLDKIL KIDPTADLEQ ILASIAPENA QDREVLLTAL RSSAEKDNPS ILALIANLEA QNGQLEPALS TINRALRRRP KVTSFILMKA NLLIALSDQE GALKWYAKSS XKYKKNLDIR LAEVRYLIQI NQPXXCS

Validated NT Sequence

gcatcaagtt gttgtaattg tggatcattt gnnnnctttt gtttcagttg ttgtaacacg gcactgaact gagttaatga tccttgcata taaagtgcct tagcgtaacg aatgccaata tttatgttat gacttagttc atatgctttg cctaaagctt ccgcagccgc ttcataatca ttttgtaaaa atgcgatata gcctagcgta tccagaatag aagcttgttc tggggcatat tctaaagcct gttcagcata ttgacgtgct tccttaagcc tacgattttg caaagctaat gtataagcat aggcgtttag atacgttggg ctattgggtt ctaactgcaa taattgtttg agtgttttat cgagtttatc gcggtcagta taagggtcta ataataaaac ttctgcatag atgagctccg gatcatctgg taagtttttg atcgcttcat ctaataaatt taatgcagct tttttattgc ccatcttctt taaaatttca gcttgagctt ggtaaagaaa gcttgcatgc tgaggataat tgactcgctc ttgggttaaa aagcgtaaag cgtcgtgtaa gttttcttgt ttatcataga tcgcaatgag attgcgtctt gaaacaatat acaaactacc atcgactaaa cnntagnnnn ctttagcagt ttcataatgt tgttttcttt cagcattaat cgctaagtag taatacgcct cattttgata ttttgcagag ttgcgtaaat nnacnngata ttgttctgct ttgtcatatt gcttannnnn nnnnnnnnnt nnnnnagcaa taaataaagc ctctnnngca tgaggattgc tttcaataat tttcnnnnat ttttcaagag caannnnngg gttgattaat ctggatgagg taacgtactt cggctaggcg aatatctagg tttttcttat atttacngct tgattttgca taccatttta aagcaccttc ctgatcactg agtgcgatta gtaaattggc tttcattaag ataaagctgg tcactttagg gcgacggcgt agtgcgcgat taatggtact taaggctggt tctaactgac cattttgtgc ttctaggttg gcaataagcg ctaagatgga tgggttgtct ttctctgcac tagaacgtaa agcggttaat aaaacttctc gatcctgtgc attttcaggt gcaatgcttg ccaaaatttg ttcaagatcg gcagttggat ctatttttaa aattttatct aaagtctcgg cagcaagctg atattcgtgt gtttttagtg caatatgcga taaataaaat agagcaggga catctttagg ctcttgaaca acccaatgtg ttgcaatatt tagggcagct tgtaagtcgt tatattcaag tgcaacgtct aatgcacgct gtttaattgt tgttgagttg ctttttatcg caagcactgt gtaattgtgc agtgctgtgg gaatatcgtc ataagctaag gcaaattcag caaccatact ctgctgtaaa gcctgatcaa atgaa

Expected Protein Sequence

MAHHHHHHRP VQETYAVHSF DQALQQSMVA EFALAYDDIP TALHNYTVLA IKSNSTTIKQ RALDVALEYN DLQAALNIAT HWVVQEPKDV PALFYLSHIA LKTHEYQLAA ETLDKILKID PTADLEQILA SIAPENAQDR EVLLTALRSS AEKDNPSILA LIANLEAQNG QLEPALSTIN RALRRRPKVT SFILMKANLL IALSDQEGAL KWYAKSSRKY KKNLDIRLAE VRYLIQINQP QLALEKLEKI IESNPHAEEA LFIAGLTSID LKQYDKAEQY LVDLRNSAKY QNEAYYYLAI NAERKQHYET AKAYYRLVDG SLYIVSRRNL IAIYDKQENL HDALRFLTQE RVNYPQHASF LYQAQAEILK KMGNKKAALN LLDEAIKNLP DDPELIYAEV LLLDPYTDRD KLDKTLKQLL QLEPNSPTYL NAYAYTLALQ NRRLKEARQY AEQALEYAPE QASILDTLGY IAFLQNDYEA AAEALGKAYE LSHNINIGIR YAKALYMQGS LTQFSAVLQQ LKQKHANDPQ LQQLDALILP TSAKKS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgtccag tgcaagagac ctatgccgtg cattcatttg atcaggcttt acagcagagt atggttgctg aatttgcctt agcttatgac gatattccca cagcactgca caattacaca gtgcttgcga taaaaagcaa ctcaacaaca attaaacagc gtgcattaga cgttgcactt gaatataacg acttacaagc tgccctaaat attgcaacac attgggttgt tcaagagcct aaagatgtcc ctgctctatt ttatttatcg catattgcac taaaaacaca cgaatatcag cttgctgccg agactttaga taaaatttta aaaatagatc caactgccga tcttgaacaa attttggcaa gcattgcacc tgaaaatgca caggatcgag aagttttatt aaccgcttta cgttctagtg cagagaaaga caacccatcc atcttagcgc ttattgccaa cctagaagca caaaatggtc agttagaacc agccttaagt accattaatc gcgcactacg ccgtcgccct aaagtgacca gctttatctt aatgaaagcc aatttactaa tcgcactcag tgatcaggaa ggtgctttaa aatggtatgc aaaatcaagc cgtaaatata agaaaaacct agatattcgc ctagccgaag tacgttacct catccagatt aatcaacccc agcttgctct tgaaaaatta gagaaaatta ttgaaagcaa tcctcatgct gaagaggctt tatttattgc tggtttaacc agtattgacc ttaagcaata tgacaaagca gaacaatatc tggttgattt acgcaactct gcaaaatatc aaaatgaggc gtattactac ttagcgatta atgctgaaag aaaacaacat tatgaaactg ctaaagctta ctaccgttta gtcgatggta gtttgtatat tgtttcaaga cgcaatctca ttgcgatcta tgataaacaa gaaaacttac acgacgcttt acgcttttta acccaagagc gagtcaatta tcctcagcat gcaagctttc tttaccaagc tcaagctgaa attttaaaga agatgggcaa taaaaaagct gcattaaatt tattagatga agcgatcaaa aacttaccag atgatccgga gctcatctat gcagaagttt tattattaga cccttatact gaccgcgata aactcgataa aacactcaaa caattattgc agttagaacc caatagccca acgtatctaa acgcctatgc ttatacatta gctttgcaaa atcgtaggct taaggaagca cgtcaatatg ctgaacaggc tttagaatat gccccagaac aagcttctat tctggatacg ctaggctata tcgcattttt acaaaatgat tatgaagcgg ctgcggaagc tttaggcaaa gcatatgaac taagtcataa cataaatatt ggcattcgtt acgctaaggc actttatatg caaggatcat taactcagtt cagtgccgtg ttacaacaac tgaaacaaaa gcacgcaaat gatccacaat tacaacaact tgatgcgtta attttaccta catctgcaaa aaagagttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for AcbaB.19551.a.B2.PW38401

PURIFICATION DATe:	12/5/2017
CONCENTRATION:	11.64mg/ml
OBSERVED MW:	53kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	39

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCGTCCAG TGCAAGAGAC CTATGCCGTG CATTCATTTG ATCAGGCTTT ACAGCAGAGT ATGGTTGCTG AATTTGCCTT AGCTTATGAC GATATTCCCA CAGCACTGCA CAATTACACA GTGCTTGCGA TAAAAAGCAA CTCAACAACA ATTAAACAGC GTGCATTAGA CGTTGCACTT GAATATAACG ACTTACAAGC TGCCCTAAAT ATTGCAACAC ATTGGGTTGT TCAAGAGCCT AAAGATGTCC CTGCTCTATT TTATTTATCG CATATTGCAC TAAAAACACA CGAATATCAG CTTGCTGCCG AGACTTTAGA TAAAATTTTA AAAATAGATC CAACTGCCGA TCTTGAACAA ATTTTGGCAA GCATTGCACC TGAAAATGCA CAGGATCGAG AAGTTTTATT AACCGCTTTA CGTTCTAGTG CAGAGAAAGA CAACCCATCC ATCTTAGCGC TTATTGCCAA CCTAGAAGCA CAAAATGGTC AGTTAGAACC AGCCTTAAGT ACCATTAATC GCGCACTACG CCGTCGCCCT AAAGTGACCA GCTTTATCTT AATGAAAGCC AATTTACTAA TCGCACTCAG TGATCAGGAA GGTGCTTTAA AATGGTATGC AAAATCAAGC CGTAAATATA AGAAAAACCT AGATATTCGC CTAGCCGAAG TACGTTACCT CATCCAGATT AATCAACCCC AGCTTGCTCT TGAAAAATTA GAGAAAATTA TTGAAAGCAA TCCTCATGCT GAAGAGGCTT TATTTATTGC TGGTTTAACC AGTATTGACC TTAAGCAATA TGACAAAGCA GAACAATATC TGGTTGATTT ACGCAACTCT GCAAAATATC AAAATGAGGC GTATTACTAC TTAGCGATTA ATGCTGAAAG AAAACAACAT TATGAAACTG CTAAAGCTTA CTACCGTTTA GTCGATGGTA GTTTGTATAT TGTTTCAAGA CGCAATCTCA TTGCGATCTA TGATAAACAA GAAAACTTAC ACGACGCTTT ACGCTTTTTA ACCCAAGAGC GAGTCAATTA TCCTCAGCAT GCAAGCTTTC TTTACCAAGC TCAAGCTGAA ATTTTAAAGA AGATGGGCAA TAAAAAAGCT GCATTAAATT TATTAGATGA AGCGATCAAA AACTTACCAG ATGATCCGGA GCTCATCTAT GCAGAAGTTT TATTATTAGA CCCTTATACT GACCGCGATA AACTCGATAA AACACTCAAA CAATTATTGC AGTTAGAACC CAATAGCCCA ACGTATCTAA ACGCCTATGC TTATACATTA GCTTTGCAAA ATCGTAGGCT TAAGGAAGCA CGTCAATATG CTGAACAGGC TTTAGAATAT GCCCCAGAAC AAGCTTCTAT TCTGGATACG CTAGGCTATA TCGCATTTTT ACAAAATGAT TATGAAGCGG CTGCGGAAGC TTTAGGCAAA GCATATGAAC TAAGTCATAA CATAAATATT GGCATTCGTT ACGCTAAGGC ACTTTATATG CAAGGATCAT TAACTCAGTT CAGTGCCGTG TTACAACAAC TGAAACAAAA GCACGCAAAT GATCCACAAT TACAACAACT TGATGCGTTA ATTTTACCTA CATCTGCAAA AAAGAGTTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT