AcbaB.17485.a
malonate decarboxylase, alpha subunit
| CENTER ID: | AcbaB.17485.a |
| ORGANISM: | Acinetobacter baumannii ATCC 17978 / NCDC KC 755 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.17485.a.B1.GE41684 | full length | 1 | 559 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.17485.a.B1.PW38403 | full length | 1 | 559 |
Structures
6MPR
DEPOSITED: 10/8/2018
DETERMINATION: XRay
CLONE: AcbaB.17485.a.B1.GE41684
PROTEIN: AcbaB.17485.a.B1.PW38403
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | ABO11849.1 |
| PGAT: | acinetobacter:A1S_1421 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGSVQNKER LWTKRRHAKQ LKLEMANQYT DGVVIPTQDI IKVLETLITP GDKVVLEGNN QKQADFLSRS LAQTNPDILH DLHMIMPSVG RSEHLDLFEK GIARKLDFSF AGPQSLRISQ LIEDGLLEIG AIHTYIELYS RLVVDLIPNV VLSAGFMADR QGNIYTGPST EDSPALIEPA AFSDGIVIVQ VNELVDDVSE LPRVDIPASW VDYVVVADQP FYIEPLFTRD PKHIKPVHVL MAMMAIRGIY EKHNVQSLNH GIGFNTAAIE LILPTYGESL GLKGKICRNW TLNPHPTLIP AIETGWVESV HCFGTELGME KYVAARPDVF FTGRDGALRS NRMMCQLAGQ YAVDLFIGAT LQVDGMGHSS TVTKGRLAGF GGAPNMGHDP RGRRHDTPAW LDMRLQGANE TETYLARGKK LVVQMVETFQ EGGKPTFVDR LDAIDVAKTA GLPLAPIMIY GDDVTHLLTE EGIAYLYKAS SQEERQAMIA AVAGVTSIGL TQDPKTTARL RREGLVVFPE DLGIRRTDAT RELLAAKNIA DLVTWSDGLY QPPAKFRSW
NT Sequence
atggaaggat cagttcagaa taaagaacga ttatggacaa agcggcgtca tgccaaacaa ctaaaactag agatggcaaa ccaatatacc gatggtgtgg tgattccaac tcaagacatt attaaagttt tggaaacctt aattacaccg ggtgacaaag ttgtactcga agggaataac caaaagcagg ctgatttttt atcgcgctct ttggctcaaa ccaatccaga tattttgcat gatttacata tgattatgcc gagcgttggg cgttcagaac atcttgatct gtttgaaaaa ggcattgcgc gtaaactcga tttttctttt gctggcccac aaagtttgcg tattagtcag ttaattgaag atggtttact agagattggt gcgattcata cctatatcga actatattca cgtttggtag tcgacctcat tccaaatgtg gtgctctcag cgggctttat ggcagaccgt caaggcaata tctatacagg cccaagtacc gaagactctc cagcactcat tgaacctgct gcttttagtg acggtatcgt gattgttcag gtcaatgaac tggtcgatga cgtctcagag ttaccccgtg tcgatattcc tgcttcttgg gtggattatg tggtggtggc ggatcagcca ttttatatcg aaccgttatt tactcgtgac ccgaaacaca ttaagccagt gcatgtgctt atggcgatga tggcaattcg cggaatttat gaaaagcata atgtgcaatc attaaaccat ggtattggtt ttaacactgc ggcaattgaa ctgattttac cgacctatgg tgaatcttta ggtttaaaag gaaaaatctg ccgaaactgg acactaaatc cgcatccgac cttaattcca gcgattgaaa caggttgggt tgaaagcgtg cattgttttg gtaccgaact cggtatggaa aaatatgttg ctgctcgtcc cgatgttttc tttacaggtc gtgatggcgc tttacgttcc aaccgcatga tgtgtcagct cgcaggtcag tacgccgttg atctatttat tggcgcaact ttgcaagttg atggtatggg ccattcttca actgtcacca aaggtcgttt ggctggtttc ggtggtgcac caaacatggg gcatgatcca cgcggacgtc gtcatgatac acctgcatgg ttagatatgc gtttgcaagg tgccaatgaa accgaaactt atttggcacg tggtaaaaag ttagttgtac aaatggtgga aaccttccaa gagggtggta agccaacttt tgtggatcgt ctcgatgcaa ttgatgtggc caaaaccgca ggcttgcctt tagccccgat catgatttat ggcgatgatg tgacccattt actgaccgaa gaaggcattg cttatctata taaagcaagc agccaagaag aacgtcaagc catgatagct gcggtggcag gtgtgaccag tattggttta actcaagacc cgaaaacaac agcacgttta agacgtgaag gtctggtggt attccctgaa gatttaggta ttcgtcgcac cgatgccaca cgtgaacttt tagcggcgaa aaatattgcc gacttggtga catggtcaga tggcttatat caaccacctg caaaattcag gagttggtaa
Details for AcbaB.17485.a.B1.GE41684
| HARVESTED ON: | 6/19/2017 |
| SEQUENCED ON: | 6/22/2017 |
| EXPECTED MW: | 63kDa |
| OBSERVED MW: | 63kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME GSVQNKERLW TKRRHAKQLK LEMANQYTDG VVIPTQDIIK VLETLITPGD KVVLEGNNQK QADFLSRSLA QTNPDILHDL HMIMPSVGRS EHLDLFEKGI ARKLDFSFAG PQSLRISQLI EDGLLEIGAI HTYIELYSRL VVDLIPNVVL SAGFMADRQG NIYTGPSTED SPALIEPAAF SDGIVIVQVN ELVDDVSELP RVDIPASWVD YVVVADQPFY IEPLFTRDPK HIKPVHVLMA MMAIRGIYEK HNVQSLNHGI GFNTAAIELI LPTYGESLGL KGKICRNWTL NPHPTLIPAI ETGWVESVHC FGTELGMEKY VAARPDVFFT GRDGALRSNR MMCQLAGQYA VDLFIGATLQ VDGMGHSSTV TKGRLAGFGG APNMGHDPRG RRHDTPAWLD MRLQGANETE TYLARGKKLV VQMVETFQEG GKPTFVDRLD AIDVAKTAGL PLAPIMIYGD DVTHLLTEEG IAYLYKASSQ EERQAMIAAV AGVTSIGLTQ DPKTTARLRR EGLVVFPEDL GIRRTDATRE LLAAKNIADL VTWSDGLYQP PAKFRSW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaag gatcagttca gaataaagaa cgattatgga caaagcggcg tcatgccaaa caactaaaac tagagatggc aaaccaatat accgatggtg tggtgattcc aactcaagac attattaaag ttttggaaac cttaattaca ccgggtgaca aagttgtact cgaagggaat aaccaaaagc aggctgattt tttatcgcgc tctttggctc aaaccaatcc agatattttg catgatttac atatgattat gccgagcgtt gggcgttcag aacatcttga tctgtttgaa aaaggcattg cgcgtaaact cgatttttct tttgctggcc cacaaagttt gcgtattagt cagttaattg aagatggttt actagagatt ggtgcgattc atacctatat cgaactatat tcacgtttgg tagtcgacct cattccaaat gtggtgctct cagcgggctt tatggcagac cgtcaaggca atatctatac aggcccaagt accgaagact ctccagcact cattgaacct gctgctttta gtgacggtat cgtgattgtt caggtcaatg aactggtcga tgacgtctca gagttacccc gtgtcgatat tcctgcttct tgggtggatt atgtggtggt ggcggatcag ccattttata tcgaaccgtt atttactcgt gacccgaaac acattaagcc agtgcatgtg cttatggcga tgatggcaat tcgcggaatt tatgaaaagc ataatgtgca atcattaaac catggtattg gttttaacac tgcggcaatt gaactgattt taccgaccta tggtgaatct ttaggtttaa aaggaaaaat ctgccgaaac tggacactaa atccgcatcc gaccttaatt ccagcgattg aaacaggttg ggttgaaagc gtgcattgtt ttggtaccga actcggtatg gaaaaatatg ttgctgctcg tcccgatgtt ttctttacag gtcgtgatgg cgctttacgt tccaaccgca tgatgtgtca gctcgcaggt cagtacgccg ttgatctatt tattggcgca actttgcaag ttgatggtat gggccattct tcaactgtca ccaaaggtcg tttggctggt ttcggtggtg caccaaacat ggggcatgat ccacgcggac gtcgtcatga tacacctgca tggttagata tgcgtttgca aggtgccaat gaaaccgaaa cttatttggc acgtggtaaa aagttagttg tacaaatggt ggaaaccttc caagagggtg gtaagccaac ttttgtggat cgtctcgatg caattgatgt ggccaaaacc gcaggcttgc ctttagcccc gatcatgatt tatggcgatg atgtgaccca tttactgacc gaagaaggca ttgcttatct atataaagca agcagccaag aagaacgtca agccatgata gctgcggtgg caggtgtgac cagtattggt ttaactcaag acccgaaaac aacagcacgt ttaagacgtg aaggtctggt ggtattccct gaagatttag gtattcgtcg caccgatgcc acacgtgaac ttttagcggc gaaaaatatt gccgacttgg tgacatggtc agatggctta tatcaaccac ctgcaaaatt caggagttgg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for AcbaB.17485.a.B1.PW38403
| PURIFICATION DATe: | 12/5/2017 |
| CONCENTRATION: | 30.13mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHME GSVQNKERLW TKRRHAKQLK LEMANQYTDG VVIPTQDIIK VLETLITPGD KVVLEGNNQK QADFLSRSLA QTNPDILHDL HMIMPSVGRS EHLDLFEKGI ARKLDFSFAG PQSLRISQLI EDGLLEIGAI HTYIELYSRL VVDLIPNVVL SAGFMADRQG NIYTGPSTED SPALIEPAAF SDGIVIVQVN ELVDDVSELP RVDIPASWVD YVVVADQPFY IEPLFTRDPK HIKPVHVLMA MMAIRGIYEK HNVQSLNHGI GFNTAAIELI LPTYGESLGL KGKICRNWTL NPHPTLIPAI ETGWVESVHC FGTELGMEKY VAARPDVFFT GRDGALRSNR MMCQLAGQYA VDLFIGATLQ VDGMGHSSTV TKGRLAGFGG APNMGHDPRG RRHDTPAWLD MRLQGANETE TYLARGKKLV VQMVETFQEG GKPTFVDRLD AIDVAKTAGL PLAPIMIYGD DVTHLLTEEG IAYLYKASSQ EERQAMIAAV AGVTSIGLTQ DPKTTARLRR EGLVVFPEDL GIRRTDATRE LLAAKNIADL VTWSDGLYQP PAKFRSW
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAG GATCAGTTCA GAATAAAGAA CGATTATGGA CAAAGCGGCG TCATGCCAAA CAACTAAAAC TAGAGATGGC AAACCAATAT ACCGATGGTG TGGTGATTCC AACTCAAGAC ATTATTAAAG TTTTGGAAAC CTTAATTACA CCGGGTGACA AAGTTGTACT CGAAGGGAAT AACCAAAAGC AGGCTGATTT TTTATCGCGC TCTTTGGCTC AAACCAATCC AGATATTTTG CATGATTTAC ATATGATTAT GCCGAGCGTT GGGCGTTCAG AACATCTTGA TCTGTTTGAA AAAGGCATTG CGCGTAAACT CGATTTTTCT TTTGCTGGCC CACAAAGTTT GCGTATTAGT CAGTTAATTG AAGATGGTTT ACTAGAGATT GGTGCGATTC ATACCTATAT CGAACTATAT TCACGTTTGG TAGTCGACCT CATTCCAAAT GTGGTGCTCT CAGCGGGCTT TATGGCAGAC CGTCAAGGCA ATATCTATAC AGGCCCAAGT ACCGAAGACT CTCCAGCACT CATTGAACCT GCTGCTTTTA GTGACGGTAT CGTGATTGTT CAGGTCAATG AACTGGTCGA TGACGTCTCA GAGTTACCCC GTGTCGATAT TCCTGCTTCT TGGGTGGATT ATGTGGTGGT GGCGGATCAG CCATTTTATA TCGAACCGTT ATTTACTCGT GACCCGAAAC ACATTAAGCC AGTGCATGTG CTTATGGCGA TGATGGCAAT TCGCGGAATT TATGAAAAGC ATAATGTGCA ATCATTAAAC CATGGTATTG GTTTTAACAC TGCGGCAATT GAACTGATTT TACCGACCTA TGGTGAATCT TTAGGTTTAA AAGGAAAAAT CTGCCGAAAC TGGACACTAA ATCCGCATCC GACCTTAATT CCAGCGATTG AAACAGGTTG GGTTGAAAGC GTGCATTGTT TTGGTACCGA ACTCGGTATG GAAAAATATG TTGCTGCTCG TCCCGATGTT TTCTTTACAG GTCGTGATGG CGCTTTACGT TCCAACCGCA TGATGTGTCA GCTCGCAGGT CAGTACGCCG TTGATCTATT TATTGGCGCA ACTTTGCAAG TTGATGGTAT GGGCCATTCT TCAACTGTCA CCAAAGGTCG TTTGGCTGGT TTCGGTGGTG CACCAAACAT GGGGCATGAT CCACGCGGAC GTCGTCATGA TACACCTGCA TGGTTAGATA TGCGTTTGCA AGGTGCCAAT GAAACCGAAA CTTATTTGGC ACGTGGTAAA AAGTTAGTTG TACAAATGGT GGAAACCTTC CAAGAGGGTG GTAAGCCAAC TTTTGTGGAT CGTCTCGATG CAATTGATGT GGCCAAAACC GCAGGCTTGC CTTTAGCCCC GATCATGATT TATGGCGATG ATGTGACCCA TTTACTGACC GAAGAAGGCA TTGCTTATCT ATATAAAGCA AGCAGCCAAG AAGAACGTCA AGCCATGATA GCTGCGGTGG CAGGTGTGAC CAGTATTGGT TTAACTCAAG ACCCGAAAAC AACAGCACGT TTAAGACGTG AAGGTCTGGT GGTATTCCCT GAAGATTTAG GTATTCGTCG CACCGATGCC ACACGTGAAC TTTTAGCGGC GAAAAATATT GCCGACTTGG TGACATGGTC AGATGGCTTA TATCAACCAC CTGCAAAATT CAGGAGTTGG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT