AcbaB.10611.a
Alcohol dehydrogenase
| CENTER ID: | AcbaB.10611.a |
| ORGANISM: | Acinetobacter baumannii ATCC 17978 / NCDC KC 755 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.10611.a.B1.GE41679 | full length | 1 | 342 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.10611.a.B1.PW38387 | full length | 1 | 342 |
Structures
6C49
DEPOSITED: 1/11/2018
DETERMINATION: XRay
CLONE: AcbaB.10611.a.B1.GE41679
PROTEIN: AcbaB.10611.a.B1.PW38387
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | ABO13825.2 |
| PATRIC ID: | fig|470.1575.peg.42 |
| PGAT: | acinetobacter:A1S_3436 |
| UniProt: | A0A1E3MC83 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNNTIQAYA AMQAGEKLVP YKFDAGELQP HQVEVKVEYC GLCHSDISVL NNEWHSTVYP VVAGHEIIGR IVALGSEAKG LQIGQRVGIG WTAESCQACD ECIGGQQVLC TGENIATIVG HAGGFADKVR AGWQWAIPLP EDLDPESAGP LLCGGITVFD PLLKHKIQAT HHVGVIGIGG LGHIAIKLLK AWGCEITAFS SNPDKTEELK AMGADHVVNS RDTEAVKAQK GKFDLLLSTV NVTLNWRAFI STLAPNGSLH FLGLTLEPVP VSVGSLIDGA KSVTGSPTGS PAALRQLLKF AARKKIAPQI ELFPMSQLNE AIERLHSGQA RYRIVLKADF AE
NT Sequence
atgagcaata atacgattca ggcttatgct gcaatgcagg caggtgaaaa actggtccca tacaaatttg atgcaggtga attacaaccg catcaggttg aggtaaaagt tgaatactgt ggtttgtgtc attctgatat ttctgtttta aataatgaat ggcactcaac cgtgtaccct gtagttgcag gccatgaaat tattggacga attgttgctt taggctcaga agccaaagga ctacaaattg ggcaacgtgt cggtattggc tggaccgccg aaagttgcca agcctgtgat gagtgtattg gcggtcagca agtactttgt acgggcgaaa atatcgcaac aatcgtggga catgcgggtg gctttgccga taaagttcgt gcaggctggc aatgggccat tccccttccc gaagatttag acccagaaag tgctggtccc ctactttgcg gcggaattac cgtttttgac ccattactca aacacaaaat acaggcaaca catcatgtgg gtgtgattgg tattggtggt ttgggtcata tagccattaa gcttcttaaa gcgtggggct gtgaaattac ggcgtttagt tctaacccag ataaaaccga agaactcaaa gcaatgggag ccgaccatgt ggtgaatagc cgcgatacag aagcggtaaa agctcaaaag ggtaaatttg atttattgct gagtaccgta aatgtgactt taaattggcg tgctttcatt tctacgctag caccaaacgg ttctttgcac tttttaggac ttacactaga acctgttcct gtttctgtag gttctctcat tgatggtgcc aaatcagtaa ctggttcacc tacaggttca cctgccgctc tacgccaact tttaaagttt gccgcacgca aaaaaattgc gcctcaaatt gagctatttc caatgtcaca gctcaatgaa gcaattgaac gtctacactc aggtcaagca cgctaccgca ttgtgcttaa agcagacttc gcagaataa
Details for AcbaB.10611.a.B1.GE41679
| HARVESTED ON: | 6/19/2017 |
| SEQUENCED ON: | 6/22/2017 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS NNTIQAYAAM QAGEKLVPYK FDAGELQPHQ VEVKVEYCGL CHSDISVLNN EWHSTVYPVV AGHEIIGRIV ALGSEAKGLQ IGQRVGIGWT AESCQACDEC IGGQQVLCTG ENIATIVGHA GGFADKVRAG WQWAIPLPED LDPESAGPLL CGGITVFDPL LKHKIQATHH VGVIGIGGLG HIAIKLLKAW GCEITAFSSN PDKTEELKAM GADHVVNSRD TEAVKAQKGK FDLLLSTVNV TLNWRAFIST LAPNGSLHFL GLTLEPVPVS VGSLIDGAKS VTGSPTGSPA ALRQLLKFAA RKKIAPQIEL FPMSQLNEAI ERLHSGQARY RIVLKADFAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ataatacgat tcaggcttat gctgcaatgc aggcaggtga aaaactggtc ccatacaaat ttgatgcagg tgaattacaa ccgcatcagg ttgaggtaaa agttgaatac tgtggtttgt gtcattctga tatttctgtt ttaaataatg aatggcactc aaccgtgtac cctgtagttg caggccatga aattattgga cgaattgttg ctttaggctc agaagccaaa ggactacaaa ttgggcaacg tgtcggtatt ggctggaccg ccgaaagttg ccaagcctgt gatgagtgta ttggcggtca gcaagtactt tgtacgggcg aaaatatcgc aacaatcgtg ggacatgcgg gtggctttgc cgataaagtt cgtgcaggct ggcaatgggc cattcccctt cccgaagatt tagacccaga aagtgctggt cccctacttt gcggcggaat taccgttttt gacccattac tcaaacacaa aatacaggca acacatcatg tgggtgtgat tggtattggt ggtttgggtc atatagccat taagcttctt aaagcgtggg gctgtgaaat tacggcgttt agttctaacc cagataaaac cgaagaactc aaagcaatgg gagccgacca tgtggtgaat agccgcgata cagaagcggt aaaagctcaa aagggtaaat ttgatttatt gctgagtacc gtaaatgtga ctttaaattg gcgtgctttc atttctacgc tagcaccaaa cggttctttg cactttttag gacttacact agaacctgtt cctgtttctg taggttctct cattgatggt gccaaatcag taactggttc acctacaggt tcacctgccg ctctacgcca acttttaaag tttgccgcac gcaaaaaaat tgcgcctcaa attgagctat ttccaatgtc acagctcaat gaagcaattg aacgtctaca ctcaggtcaa gcacgctacc gcattgtgct taaagcagac ttcgcagaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for AcbaB.10611.a.B1.PW38387
| PURIFICATION DATe: | 11/6/2017 |
| CONCENTRATION: | 22.95mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
IMAC->SEC
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMS NNTIQAYAAM QAGEKLVPYK FDAGELQPHQ VEVKVEYCGL CHSDISVLNN EWHSTVYPVV AGHEIIGRIV ALGSEAKGLQ IGQRVGIGWT AESCQACDEC IGGQQVLCTG ENIATIVGHA GGFADKVRAG WQWAIPLPED LDPESAGPLL CGGITVFDPL LKHKIQATHH VGVIGIGGLG HIAIKLLKAW GCEITAFSSN PDKTEELKAM GADHVVNSRD TEAVKAQKGK FDLLLSTVNV TLNWRAFIST LAPNGSLHFL GLTLEPVPVS VGSLIDGAKS VTGSPTGSPA ALRQLLKFAA RKKIAPQIEL FPMSQLNEAI ERLHSGQARY RIVLKADFAE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA ATAATACGAT TCAGGCTTAT GCTGCAATGC AGGCAGGTGA AAAACTGGTC CCATACAAAT TTGATGCAGG TGAATTACAA CCGCATCAGG TTGAGGTAAA AGTTGAATAC TGTGGTTTGT GTCATTCTGA TATTTCTGTT TTAAATAATG AATGGCACTC AACCGTGTAC CCTGTAGTTG CAGGCCATGA AATTATTGGA CGAATTGTTG CTTTAGGCTC AGAAGCCAAA GGACTACAAA TTGGGCAACG TGTCGGTATT GGCTGGACCG CCGAAAGTTG CCAAGCCTGT GATGAGTGTA TTGGCGGTCA GCAAGTACTT TGTACGGGCG AAAATATCGC AACAATCGTG GGACATGCGG GTGGCTTTGC CGATAAAGTT CGTGCAGGCT GGCAATGGGC CATTCCCCTT CCCGAAGATT TAGACCCAGA AAGTGCTGGT CCCCTACTTT GCGGCGGAAT TACCGTTTTT GACCCATTAC TCAAACACAA AATACAGGCA ACACATCATG TGGGTGTGAT TGGTATTGGT GGTTTGGGTC ATATAGCCAT TAAGCTTCTT AAAGCGTGGG GCTGTGAAAT TACGGCGTTT AGTTCTAACC CAGATAAAAC CGAAGAACTC AAAGCAATGG GAGCCGACCA TGTGGTGAAT AGCCGCGATA CAGAAGCGGT AAAAGCTCAA AAGGGTAAAT TTGATTTATT GCTGAGTACC GTAAATGTGA CTTTAAATTG GCGTGCTTTC ATTTCTACGC TAGCACCAAA CGGTTCTTTG CACTTTTTAG GACTTACACT AGAACCTGTT CCTGTTTCTG TAGGTTCTCT CATTGATGGT GCCAAATCAG TAACTGGTTC ACCTACAGGT TCACCTGCCG CTCTACGCCA ACTTTTAAAG TTTGCCGCAC GCAAAAAAAT TGCGCCTCAA ATTGAGCTAT TTCCAATGTC ACAGCTCAAT GAAGCAATTG AACGTCTACA CTCAGGTCAA GCACGCTACC GCATTGTGCT TAAAGCAGAC TTCGCAGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT