AcbaB.10201.a
Methionine--tRNA ligase (EC 6.1.1.10) (Methionyl-tRNA synthetase)
| CENTER ID: | AcbaB.10201.a |
| ORGANISM: | Acinetobacter baumannii ATCC 17978 / NCDC KC 755 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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You may order multiple materials at a time at no cost to you, as this contract is funded
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.10201.a.A1.GE39848 | full length | 1 | 687 | ||
| AcbaB.10201.a.A2.GE39849 | truncation 1 | 1 | 546 | ||
| AcbaB.10201.a.A3.GE39850 | truncation 2 | 1 | 570 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AcbaB.10201.a.A1.PW37926 | full length | 1 | 687 | ||
| AcbaB.10201.a.A2.PW37927 | truncation 1 | 1 | 546 | ||
| AcbaB.10201.a.A2.PW37928 | truncation 1 | 1 | 546 |
Structures
5URB
DEPOSITED: 2/10/2017
DETERMINATION: XRay
CLONE: AcbaB.10201.a.A2.GE39849
PROTEIN: AcbaB.10201.a.A2.PW37928
Publications by SSGCID
Towards a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PMID: 31930600; PMCID: PMC7020997
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| GenBank: | ABO11218.2 |
| PATRIC ID: | fig|470.1314.peg.566 |
| PGAT: | acinetobacter:A1S_0778 |
| UniProt: | A0A0D5YKJ7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKILVTNAL PYANGPIHMG HLLGYIQADI WVRAMRAMGH DVTYVCADDA HGTAIMLRAE ANGISPEEQI ANVQKEHIRD FDGFGVHFDH YDSTHSDANK ARSTDIYIKN REAGNIAVRP VTQLFDPEKG MFLSDRFIKG TCPKCKSEDQ YGDSCEVCGT TYNATELLNP RSTLSGATPV EKSSDHYFFK LPNFAEYLQK WTRDEGRLPL SIANKLDEWF EAGLADWDIS RDAPYFGFEI PDAPNKYFYV WVDAPIGYMS SFENYIKTKR PDLNFDDFWK KDSQNEVYHF IGKDIVYFHA LFWPAMLEGA NYRTPTGLFV NGFLTVNGQK MSKSRGTFIK AETYLQHLNP EYLRYYFASK LSDKVEDSDL NLDDFVQKVN SDLVGKVVNI ASRCAKFINS SFNNTLSSTC AESDLVQSFI DAGDSIAAAY EAREFSTAIR EIMALADRAN QYIDEKKPWA LAKQEGQEQQ VLDVCSVGIN LFRQLAVYLA PVLPTLAQQV QDFLKLESFD FESRKQILVS HEIAQFQPLM QRVDPKAVAA MVDASKESLG TPAPQATKAA KKEKSAEKKA APTPAVGEAE IIGIEDFLKV DLRVAQVVEA GTVEGSDKLL QLTLDVGEAE PRNVFSGIRS QYAPEDLKGK LVVMVANLAP RKMRFGISNG MVLAAGNGEG IFIISPDSGA KPGDKVS
NT Sequence
gtgcgtaaaa ttttagtcac caatgccctc ccttatgcca atggtccaat tcatatgggt catttactcg gttatatcca ggcagacatc tgggttcgtg ccatgcgggc aatgggtcat gacgtgactt atgtatgtgc ggatgatgct cacggtacag caattatgct acgtgccgaa gcaaatggta ttagcccaga agagcaaatc gcgaatgttc aaaaagaaca catccgtgat tttgacggtt ttggcgtaca tttcgatcat tatgattcaa ctcatagtga tgcaaacaaa gctcgttcaa cagatatcta tattaaaaac cgtgaagccg gaaatattgc agttcgacct gtaactcaat tatttgatcc ggaaaaaggt atgttcttgt ctgaccgttt cattaagggt acctgtccaa aatgtaagtc agaggatcaa tacggcgact catgtgaagt gtgtggtaca acttataatg cgactgagtt actcaacccg cgttcgactt taagtggtgc aacaccagtt gaaaaatcat cagatcacta tttcttcaag cttccaaact ttgctgaata tttgcaaaaa tggacccgcg atgaaggtcg cttacctctt tcaattgcta acaaattgga tgaatggttt gaagcaggtt tagcagactg ggatatttct cgtgatgctc cttattttgg ttttgaaatt ccggatgcgc caaataaata tttctatgtg tgggtagatg ccccaatcgg ttatatgtct agttttgaaa actacatcaa aactaagcgc ccagacttga actttgatga tttctggaag aaagatagtc aaaatgaggt ttaccacttt attggtaaag acattgttta cttccatgct ttattctggc ctgcgatgct tgaaggggcg aactaccgca ctccaacagg cttattcgta aatggtttct tgactgtaaa tggacaaaaa atgtctaagt ctcgcggtac tttcattaaa gccgagacgt acttacaaca tctaaaccca gagtatttac gctattactt tgcttctaaa ctttcagata aagttgaaga ctctgacttg aaccttgatg actttgtgca gaaagtaaat tcagatttag tcggtaaagt agttaatatt gctagtcgct gtgctaagtt cattaacagc agctttaaca atacgctatc aagtacatgt gctgaatctg acttagtaca aagctttatt gatgctggtg attcaattgc tgctgcatat gaagcgcgtg aattctctac ggcgatccgt gaaattatgg cgcttgctga ccgcgcaaac caatatattg atgagaaaaa accttgggct cttgctaaac aagaaggtca agaacaacaa gttcttgatg tatgctctgt cggtatcaac ttattccgtc aattagcagt gtacttagct ccagttctgc caactttagc tcagcaagtt caagatttct taaaacttga aagcttcgat ttcgagtcac gtaaacaaat tcttgtaagc catgagattg cacagttcca acctcttatg cagcgtgttg atccaaaagc tgttgcagcc atggttgatg cttctaaaga gtctttaggt actcctgcgc ctcaagcgac taaagcagcg aaaaaagaga aatctgccga gaaaaaagca gcccctactc cagcagttgg tgaagctgaa attattggta ttgaagactt cttaaaagtt gacttacgtg ttgctcaggt cgttgaggca ggtacagttg agggttcaga taaattactt caactgacgt tagacgtagg tgaagctgaa ccacgtaatg tatttagtgg tattcgtagt caatatgcgc cagaagacct aaaaggaaaa ttggtcgtta tggtggctaa ccttgcacca cgtaaaatgc gttttggtat ttcaaacggt atggtacttg ctgccggaaa tggtgaaggt attttcatta tttcaccaga tagcggtgca aaacctggcg ataaagtttc ttaa
Details for AcbaB.10201.a.A1.GE39848
| HARVESTED ON: | 1/26/2016 |
| SEQUENCED ON: | 2/12/2016 |
| EXPECTED MW: | 79kDa |
| OBSERVED MW: | 79kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASKESL GTPAPQATKA AKKEKSAEKK AAPTPAVGEA EIIGIEDFLK VDLRVAQVVE AGTVEGSDKL LQLTLDVGEA EPRNVFSGIR SQYAPEDLKG KLVVMVANLA PRKMRFGISN GMVLAAGNGE GIFIISPDSG AKPGDKVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcgtaaaatt ttagtcacca atgccctccc ttatgccaat ggtccaattc atatgggtca tttactcggt tatatccagg cagacatctg ggttcgtgcc atgcgggcaa tgggtcatga cgtgacttat gtatgtgcgg atgatgctca cggtacagca attatgctac gtgccgaagc aaatggtatt agcccagaag agcaaatcgc gaatgttcaa aaagaacaca tccgtgattt tgacggtttt ggcgtacatt tcgatcatta tgattcaact catagtgatg caaacaaagc tcgttcaaca gatatctata ttaaaaaccg tgaagccgga aatattgcag ttcgacctgt aactcaatta tttgatccgg aaaaaggtat gttcttgtct gaccgtttca ttaagggtac ctgtccaaaa tgtaagtcag aggatcaata cggcgactca tgtgaagtgt gtggtacaac ttataatgcg actgagttac tcaacccgcg ttcgacttta agtggtgcaa caccagttga aaaatcatca gatcactatt tcttcaagct tccaaacttt gctgaatatt tgcaaaaatg gacccgcgat gaaggtcgct tacctctttc aattgctaac aaattggatg aatggtttga agcaggttta gcagactggg atatttctcg tgatgctcct tattttggtt ttgaaattcc ggatgcgcca aataaatatt tctatgtgtg ggtagatgcc ccaatcggtt atatgtctag ttttgaaaac tacatcaaaa ctaagcgccc agacttgaac tttgatgatt tctggaagaa agatagtcaa aatgaggttt accactttat tggtaaagac attgtttact tccatgcttt attctggcct gcgatgcttg aaggggcgaa ctaccgcact ccaacaggct tattcgtaaa tggtttcttg actgtaaatg gacaaaaaat gtctaagtct cgcggtactt tcattaaagc cgagacgtac ttacaacatc taaacccaga gtatttacgc tattactttg cttctaaact ttcagataaa gttgaagact ctgacttgaa ccttgatgac tttgtgcaga aagtaaattc agatttagtc ggtaaagtag ttaatattgc tagtcgctgt gctaagttca ttaacagcag ctttaacaat acgctatcaa gtacatgtgc tgaatctgac ttagtacaaa gctttattga tgctggtgat tcaattgctg ctgcatatga agcgcgtgaa ttctctacgg cgatccgtga aattatggcg cttgctgacc gcgcaaacca atatattgat gagaaaaaac cttgggctct tgctaaacaa gaaggtcaag aacaacaagt tcttgatgta tgctctgtcg gtatcaactt attccgtcaa ttagcagtgt acttagctcc agttctgcca actttagctc agcaagttca agatttctta aaacttgaaa gcttcgattt cgagtcacgt aaacaaattc ttgtaagcca tgagattgca cagttccaac ctcttatgca gcgtgttgat ccaaaagctg ttgcagccat ggttgatgct tctaaagagt ctttaggtac tcctgcgcct caagcgacta aagcagcgaa aaaagagaaa tctgccgaga aaaaagcagc ccctactcca gcagttggtg aagctgaaat tattggtatt gaagacttct taaaagttga cttacgtgtt gctcaggtcg ttgaggcagg tacagttgag ggttcagata aattacttca actgacgtta gacgtaggtg aagctgaacc acgtaatgta tttagtggta ttcgtagtca atatgcgcca gaagacctaa aaggaaaatt ggtcgttatg gtggctaacc ttgcaccacg taaaatgcgt tttggtattt caaacggtat ggtacttgct gccggaaatg gtgaaggtat tttcattatt tcaccagata gcggtgcaaa acctggcgat aaagtttctt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for AcbaB.10201.a.A2.GE39849
| HARVESTED ON: | 1/26/2016 |
| SEQUENCED ON: | 2/12/2016 |
| EXPECTED MW: | 64kDa |
| OBSERVED MW: | 62kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcgtaaaatt ttagtcacca atgccctccc ttatgccaat ggtccaattc atatgggtca tttactcggt tatatccagg cagacatctg ggttcgtgcc atgcgggcaa tgggtcatga cgtgacttat gtatgtgcgg atgatgctca cggtacagca attatgctac gtgccgaagc aaatggtatt agcccagaag agcaaatcgc gaatgttcaa aaagaacaca tccgtgattt tgacggtttt ggcgtacatt tcgatcatta tgattcaact catagtgatg caaacaaagc tcgttcaaca gatatctata ttaaaaaccg tgaagccgga aatattgcag ttcgacctgt aactcaatta tttgatccgg aaaaaggtat gttcttgtct gaccgtttca ttaagggtac ctgtccaaaa tgtaagtcag aggatcaata cggcgactca tgtgaagtgt gtggtacaac ttataatgcg actgagttac tcaacccgcg ttcgacttta agtggtgcaa caccagttga aaaatcatca gatcactatt tcttcaagct tccaaacttt gctgaatatt tgcaaaaatg gacccgcgat gaaggtcgct tacctctttc aattgctaac aaattggatg aatggtttga agcaggttta gcagactggg atatttctcg tgatgctcct tattttggtt ttgaaattcc ggatgcgcca aataaatatt tctatgtgtg ggtagatgcc ccaatcggtt atatgtctag ttttgaaaac tacatcaaaa ctaagcgccc agacttgaac tttgatgatt tctggaagaa agatagtcaa aatgaggttt accactttat tggtaaagac attgtttact tccatgcttt attctggcct gcgatgcttg aaggggcgaa ctaccgcact ccaacaggct tattcgtaaa tggtttcttg actgtaaatg gacaaaaaat gtctaagtct cgcggtactt tcattaaagc cgagacgtac ttacaacatc taaacccaga gtatttacgc tattactttg cttctaaact ttcagataaa gttgaagact ctgacttgaa ccttgatgac tttgtgcaga aagtaaattc agatttagtc ggtaaagtag ttaatattgc tagtcgctgt gctaagttca ttaacagcag ctttaacaat acgctatcaa gtacatgtgc tgaatctgac ttagtacaaa gctttattga tgctggtgat tcaattgctg ctgcatatga agcgcgtgaa ttctctacgg cgatccgtga aattatggcg cttgctgacc gcgcaaacca atatattgat gagaaaaaac cttgggctct tgctaaacaa gaaggtcaag aacaacaagt tcttgatgta tgctctgtcg gtatcaactt attccgtcaa ttagcagtgt acttagctcc agttctgcca actttagctc agcaagttca agatttctta aaacttgaaa gcttcgattt cgagtcacgt aaacaaattc ttgtaagcca tgagattgca cagttccaac ctcttatgca gcgtgttgat ccaaaagctg ttgcagccat ggttgatgct tctaaataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for AcbaB.10201.a.A3.GE39850
| HARVESTED ON: | 1/26/2016 |
| SEQUENCED ON: | 2/12/2016 |
| EXPECTED MW: | 67kDa |
| OBSERVED MW: | 67kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASKESL GTPAPQATKA AKKEKSAEKK A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcgtaaaatt ttagtcacca atgccctccc ttatgccaat ggtccaattc atatgggtca tttactcggt tatatccagg cagacatctg ggttcgtgcc atgcgggcaa tgggtcatga cgtgacttat gtatgtgcgg atgatgctca cggtacagca attatgctac gtgccgaagc aaatggtatt agcccagaag agcaaatcgc gaatgttcaa aaagaacaca tccgtgattt tgacggtttt ggcgtacatt tcgatcatta tgattcaact catagtgatg caaacaaagc tcgttcaaca gatatctata ttaaaaaccg tgaagccgga aatattgcag ttcgacctgt aactcaatta tttgatccgg aaaaaggtat gttcttgtct gaccgtttca ttaagggtac ctgtccaaaa tgtaagtcag aggatcaata cggcgactca tgtgaagtgt gtggtacaac ttataatgcg actgagttac tcaacccgcg ttcgacttta agtggtgcaa caccagttga aaaatcatca gatcactatt tcttcaagct tccaaacttt gctgaatatt tgcaaaaatg gacccgcgat gaaggtcgct tacctctttc aattgctaac aaattggatg aatggtttga agcaggttta gcagactggg atatttctcg tgatgctcct tattttggtt ttgaaattcc ggatgcgcca aataaatatt tctatgtgtg ggtagatgcc ccaatcggtt atatgtctag ttttgaaaac tacatcaaaa ctaagcgccc agacttgaac tttgatgatt tctggaagaa agatagtcaa aatgaggttt accactttat tggtaaagac attgtttact tccatgcttt attctggcct gcgatgcttg aaggggcgaa ctaccgcact ccaacaggct tattcgtaaa tggtttcttg actgtaaatg gacaaaaaat gtctaagtct cgcggtactt tcattaaagc cgagacgtac ttacaacatc taaacccaga gtatttacgc tattactttg cttctaaact ttcagataaa gttgaagact ctgacttgaa ccttgatgac tttgtgcaga aagtaaattc agatttagtc ggtaaagtag ttaatattgc tagtcgctgt gctaagttca ttaacagcag ctttaacaat acgctatcaa gtacatgtgc tgaatctgac ttagtacaaa gctttattga tgctggtgat tcaattgctg ctgcatatga agcgcgtgaa ttctctacgg cgatccgtga aattatggcg cttgctgacc gcgcaaacca atatattgat gagaaaaaac cttgggctct tgctaaacaa gaaggtcaag aacaacaagt tcttgatgta tgctctgtcg gtatcaactt attccgtcaa ttagcagtgt acttagctcc agttctgcca actttagctc agcaagttca agatttctta aaacttgaaa gcttcgattt cgagtcacgt aaacaaattc ttgtaagcca tgagattgca cagttccaac ctcttatgca gcgtgttgat ccaaaagctg ttgcagccat ggttgatgct tctaaagagt ctttaggtac tcctgcgcct caagcgacta aagcagcgaa aaaagagaaa tctgccgaga aaaaagcata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for AcbaB.10201.a.A1.PW37926
| PURIFICATION DATe: | 7/11/2016 |
| CONCENTRATION: | 33.5mg/ml |
| OBSERVED MW: | 78kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASKESL GTPAPQATKA AKKEKSAEKK AAPTPAVGEA EIIGIEDFLK VDLRVAQVVE AGTVEGSDKL LQLTLDVGEA EPRNVFSGIR SQYAPEDLKG KLVVMVANLA PRKMRFGISN GMVLAAGNGE GIFIISPDSG AKPGDKVS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GCGTAAAATT TTAGTCACCA ATGCCCTCCC TTATGCCAAT GGTCCAATTC ATATGGGTCA TTTACTCGGT TATATCCAGG CAGACATCTG GGTTCGTGCC ATGCGGGCAA TGGGTCATGA CGTGACTTAT GTATGTGCGG ATGATGCTCA CGGTACAGCA ATTATGCTAC GTGCCGAAGC AAATGGTATT AGCCCAGAAG AGCAAATCGC GAATGTTCAA AAAGAACACA TCCGTGATTT TGACGGTTTT GGCGTACATT TCGATCATTA TGATTCAACT CATAGTGATG CAAACAAAGC TCGTTCAACA GATATCTATA TTAAAAACCG TGAAGCCGGA AATATTGCAG TTCGACCTGT AACTCAATTA TTTGATCCGG AAAAAGGTAT GTTCTTGTCT GACCGTTTCA TTAAGGGTAC CTGTCCAAAA TGTAAGTCAG AGGATCAATA CGGCGACTCA TGTGAAGTGT GTGGTACAAC TTATAATGCG ACTGAGTTAC TCAACCCGCG TTCGACTTTA AGTGGTGCAA CACCAGTTGA AAAATCATCA GATCACTATT TCTTCAAGCT TCCAAACTTT GCTGAATATT TGCAAAAATG GACCCGCGAT GAAGGTCGCT TACCTCTTTC AATTGCTAAC AAATTGGATG AATGGTTTGA AGCAGGTTTA GCAGACTGGG ATATTTCTCG TGATGCTCCT TATTTTGGTT TTGAAATTCC GGATGCGCCA AATAAATATT TCTATGTGTG GGTAGATGCC CCAATCGGTT ATATGTCTAG TTTTGAAAAC TACATCAAAA CTAAGCGCCC AGACTTGAAC TTTGATGATT TCTGGAAGAA AGATAGTCAA AATGAGGTTT ACCACTTTAT TGGTAAAGAC ATTGTTTACT TCCATGCTTT ATTCTGGCCT GCGATGCTTG AAGGGGCGAA CTACCGCACT CCAACAGGCT TATTCGTAAA TGGTTTCTTG ACTGTAAATG GACAAAAAAT GTCTAAGTCT CGCGGTACTT TCATTAAAGC CGAGACGTAC TTACAACATC TAAACCCAGA GTATTTACGC TATTACTTTG CTTCTAAACT TTCAGATAAA GTTGAAGACT CTGACTTGAA CCTTGATGAC TTTGTGCAGA AAGTAAATTC AGATTTAGTC GGTAAAGTAG TTAATATTGC TAGTCGCTGT GCTAAGTTCA TTAACAGCAG CTTTAACAAT ACGCTATCAA GTACATGTGC TGAATCTGAC TTAGTACAAA GCTTTATTGA TGCTGGTGAT TCAATTGCTG CTGCATATGA AGCGCGTGAA TTCTCTACGG CGATCCGTGA AATTATGGCG CTTGCTGACC GCGCAAACCA ATATATTGAT GAGAAAAAAC CTTGGGCTCT TGCTAAACAA GAAGGTCAAG AACAACAAGT TCTTGATGTA TGCTCTGTCG GTATCAACTT ATTCCGTCAA TTAGCAGTGT ACTTAGCTCC AGTTCTGCCA ACTTTAGCTC AGCAAGTTCA AGATTTCTTA AAACTTGAAA GCTTCGATTT CGAGTCACGT AAACAAATTC TTGTAAGCCA TGAGATTGCA CAGTTCCAAC CTCTTATGCA GCGTGTTGAT CCAAAAGCTG TTGCAGCCAT GGTTGATGCT TCTAAAGAGT CTTTAGGTAC TCCTGCGCCT CAAGCGACTA AAGCAGCGAA AAAAGAGAAA TCTGCCGAGA AAAAAGCAGC CCCTACTCCA GCAGTTGGTG AAGCTGAAAT TATTGGTATT GAAGACTTCT TAAAAGTTGA CTTACGTGTT GCTCAGGTCG TTGAGGCAGG TACAGTTGAG GGTTCAGATA AATTACTTCA ACTGACGTTA GACGTAGGTG AAGCTGAACC ACGTAATGTA TTTAGTGGTA TTCGTAGTCA ATATGCGCCA GAAGACCTAA AAGGAAAATT GGTCGTTATG GTGGCTAACC TTGCACCACG TAAAATGCGT TTTGGTATTT CAAACGGTAT GGTACTTGCT GCCGGAAATG GTGAAGGTAT TTTCATTATT TCACCAGATA GCGGTGCAAA ACCTGGCGAT AAAGTTTCTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA
Details for AcbaB.10201.a.A2.PW37927
| PURIFICATION DATe: | 7/11/2016 |
| CONCENTRATION: | 23.5mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GCGTAAAATT TTAGTCACCA ATGCCCTCCC TTATGCCAAT GGTCCAATTC ATATGGGTCA TTTACTCGGT TATATCCAGG CAGACATCTG GGTTCGTGCC ATGCGGGCAA TGGGTCATGA CGTGACTTAT GTATGTGCGG ATGATGCTCA CGGTACAGCA ATTATGCTAC GTGCCGAAGC AAATGGTATT AGCCCAGAAG AGCAAATCGC GAATGTTCAA AAAGAACACA TCCGTGATTT TGACGGTTTT GGCGTACATT TCGATCATTA TGATTCAACT CATAGTGATG CAAACAAAGC TCGTTCAACA GATATCTATA TTAAAAACCG TGAAGCCGGA AATATTGCAG TTCGACCTGT AACTCAATTA TTTGATCCGG AAAAAGGTAT GTTCTTGTCT GACCGTTTCA TTAAGGGTAC CTGTCCAAAA TGTAAGTCAG AGGATCAATA CGGCGACTCA TGTGAAGTGT GTGGTACAAC TTATAATGCG ACTGAGTTAC TCAACCCGCG TTCGACTTTA AGTGGTGCAA CACCAGTTGA AAAATCATCA GATCACTATT TCTTCAAGCT TCCAAACTTT GCTGAATATT TGCAAAAATG GACCCGCGAT GAAGGTCGCT TACCTCTTTC AATTGCTAAC AAATTGGATG AATGGTTTGA AGCAGGTTTA GCAGACTGGG ATATTTCTCG TGATGCTCCT TATTTTGGTT TTGAAATTCC GGATGCGCCA AATAAATATT TCTATGTGTG GGTAGATGCC CCAATCGGTT ATATGTCTAG TTTTGAAAAC TACATCAAAA CTAAGCGCCC AGACTTGAAC TTTGATGATT TCTGGAAGAA AGATAGTCAA AATGAGGTTT ACCACTTTAT TGGTAAAGAC ATTGTTTACT TCCATGCTTT ATTCTGGCCT GCGATGCTTG AAGGGGCGAA CTACCGCACT CCAACAGGCT TATTCGTAAA TGGTTTCTTG ACTGTAAATG GACAAAAAAT GTCTAAGTCT CGCGGTACTT TCATTAAAGC CGAGACGTAC TTACAACATC TAAACCCAGA GTATTTACGC TATTACTTTG CTTCTAAACT TTCAGATAAA GTTGAAGACT CTGACTTGAA CCTTGATGAC TTTGTGCAGA AAGTAAATTC AGATTTAGTC GGTAAAGTAG TTAATATTGC TAGTCGCTGT GCTAAGTTCA TTAACAGCAG CTTTAACAAT ACGCTATCAA GTACATGTGC TGAATCTGAC TTAGTACAAA GCTTTATTGA TGCTGGTGAT TCAATTGCTG CTGCATATGA AGCGCGTGAA TTCTCTACGG CGATCCGTGA AATTATGGCG CTTGCTGACC GCGCAAACCA ATATATTGAT GAGAAAAAAC CTTGGGCTCT TGCTAAACAA GAAGGTCAAG AACAACAAGT TCTTGATGTA TGCTCTGTCG GTATCAACTT ATTCCGTCAA TTAGCAGTGT ACTTAGCTCC AGTTCTGCCA ACTTTAGCTC AGCAAGTTCA AGATTTCTTA AAACTTGAAA GCTTCGATTT CGAGTCACGT AAACAAATTC TTGTAAGCCA TGAGATTGCA CAGTTCCAAC CTCTTATGCA GCGTGTTGAT CCAAAAGCTG TTGCAGCCAT GGTTGATGCT TCTAAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA
Details for AcbaB.10201.a.A2.PW37928
| PURIFICATION DATe: | 7/11/2016 |
| CONCENTRATION: | 40mg/ml |
| OBSERVED MW: | 64kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRKILVTNA LPYANGPIHM GHLLGYIQAD IWVRAMRAMG HDVTYVCADD AHGTAIMLRA EANGISPEEQ IANVQKEHIR DFDGFGVHFD HYDSTHSDAN KARSTDIYIK NREAGNIAVR PVTQLFDPEK GMFLSDRFIK GTCPKCKSED QYGDSCEVCG TTYNATELLN PRSTLSGATP VEKSSDHYFF KLPNFAEYLQ KWTRDEGRLP LSIANKLDEW FEAGLADWDI SRDAPYFGFE IPDAPNKYFY VWVDAPIGYM SSFENYIKTK RPDLNFDDFW KKDSQNEVYH FIGKDIVYFH ALFWPAMLEG ANYRTPTGLF VNGFLTVNGQ KMSKSRGTFI KAETYLQHLN PEYLRYYFAS KLSDKVEDSD LNLDDFVQKV NSDLVGKVVN IASRCAKFIN SSFNNTLSST CAESDLVQSF IDAGDSIAAA YEAREFSTAI REIMALADRA NQYIDEKKPW ALAKQEGQEQ QVLDVCSVGI NLFRQLAVYL APVLPTLAQQ VQDFLKLESF DFESRKQILV SHEIAQFQPL MQRVDPKAVA AMVDASK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGGT GCGTAAAATT TTAGTCACCA ATGCCCTCCC TTATGCCAAT GGTCCAATTC ATATGGGTCA TTTACTCGGT TATATCCAGG CAGACATCTG GGTTCGTGCC ATGCGGGCAA TGGGTCATGA CGTGACTTAT GTATGTGCGG ATGATGCTCA CGGTACAGCA ATTATGCTAC GTGCCGAAGC AAATGGTATT AGCCCAGAAG AGCAAATCGC GAATGTTCAA AAAGAACACA TCCGTGATTT TGACGGTTTT GGCGTACATT TCGATCATTA TGATTCAACT CATAGTGATG CAAACAAAGC TCGTTCAACA GATATCTATA TTAAAAACCG TGAAGCCGGA AATATTGCAG TTCGACCTGT AACTCAATTA TTTGATCCGG AAAAAGGTAT GTTCTTGTCT GACCGTTTCA TTAAGGGTAC CTGTCCAAAA TGTAAGTCAG AGGATCAATA CGGCGACTCA TGTGAAGTGT GTGGTACAAC TTATAATGCG ACTGAGTTAC TCAACCCGCG TTCGACTTTA AGTGGTGCAA CACCAGTTGA AAAATCATCA GATCACTATT TCTTCAAGCT TCCAAACTTT GCTGAATATT TGCAAAAATG GACCCGCGAT GAAGGTCGCT TACCTCTTTC AATTGCTAAC AAATTGGATG AATGGTTTGA AGCAGGTTTA GCAGACTGGG ATATTTCTCG TGATGCTCCT TATTTTGGTT TTGAAATTCC GGATGCGCCA AATAAATATT TCTATGTGTG GGTAGATGCC CCAATCGGTT ATATGTCTAG TTTTGAAAAC TACATCAAAA CTAAGCGCCC AGACTTGAAC TTTGATGATT TCTGGAAGAA AGATAGTCAA AATGAGGTTT ACCACTTTAT TGGTAAAGAC ATTGTTTACT TCCATGCTTT ATTCTGGCCT GCGATGCTTG AAGGGGCGAA CTACCGCACT CCAACAGGCT TATTCGTAAA TGGTTTCTTG ACTGTAAATG GACAAAAAAT GTCTAAGTCT CGCGGTACTT TCATTAAAGC CGAGACGTAC TTACAACATC TAAACCCAGA GTATTTACGC TATTACTTTG CTTCTAAACT TTCAGATAAA GTTGAAGACT CTGACTTGAA CCTTGATGAC TTTGTGCAGA AAGTAAATTC AGATTTAGTC GGTAAAGTAG TTAATATTGC TAGTCGCTGT GCTAAGTTCA TTAACAGCAG CTTTAACAAT ACGCTATCAA GTACATGTGC TGAATCTGAC TTAGTACAAA GCTTTATTGA TGCTGGTGAT TCAATTGCTG CTGCATATGA AGCGCGTGAA TTCTCTACGG CGATCCGTGA AATTATGGCG CTTGCTGACC GCGCAAACCA ATATATTGAT GAGAAAAAAC CTTGGGCTCT TGCTAAACAA GAAGGTCAAG AACAACAAGT TCTTGATGTA TGCTCTGTCG GTATCAACTT ATTCCGTCAA TTAGCAGTGT ACTTAGCTCC AGTTCTGCCA ACTTTAGCTC AGCAAGTTCA AGATTTCTTA AAACTTGAAA GCTTCGATTT CGAGTCACGT AAACAAATTC TTGTAAGCCA TGAGATTGCA CAGTTCCAAC CTCTTATGCA GCGTGTTGAT CCAAAAGCTG TTGCAGCCAT GGTTGATGCT TCTAAATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA