AcbaB.10201.a
Methionine--tRNA ligase (EC 6.1.1.10) (Methionyl-tRNA synthetase)
CENTER ID:
AcbaB.10201.a
ORGANISM:
Acinetobacter baumannii ATCC 17978 / NCDC KC 755
ASSOCIATED DISEASE:
CURRENT STATUS:
in PDB
COMMUNITY REQUEST:
True
NIH RISK GROUP:
2
SELECT AGENT:
False
NIH PRIORITY
pathogens category:
unclassified
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO
AA
START
AA
STOP
ORDER
MATERIAL
AcbaB.10201.a.A1.GE39848
full length
info
1
687
AcbaB.10201.a.A1.GE39848
AcbaB.10201.a.A2.GE39849
truncation 1
info
1
546
AcbaB.10201.a.A2.GE39849
AcbaB.10201.a.A3.GE39850
truncation 2
info
1
570
AcbaB.10201.a.A3.GE39850
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis . Get sequence information
using the button in the "info" column.
Proteins
CENTER REFERENCE ID
DOMAIN/REGION DESCRIPTION
INFO
AA START
AA STOP
ORDER MATERIAL
AcbaB.10201.a.A1.PW37926
full length
info
1
687
PW37926
AcbaB.10201.a.A2.PW37927
truncation 1
info
1
546
PW37927
AcbaB.10201.a.A2.PW37928
truncation 1
info
1
546
PW37928
Structures
5URB
DEPOSITED: 2/10/2017
DETERMINATION: XRay
CLONE: AcbaB.10201.a.A2.GE39849
PROTEIN: AcbaB.10201.a.A2.PW37928
Publications by SSGCID
Toward a structome of Acinetobacter baumannii drug targets
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Lorimer DD, Myler PJ, Phan I, Staker B, Subramanian S, Van Voorhis WC, Craig JC, Chun IKH, Barrett KF, Prentice RA, Tillery L, Shek P
Protein Sci. - 2020
volume 29, issue 3, pages 789-802
PDB:
4YL5 ,
4YWR ,
4ZIY ,
4ZJU ,
4ZN6 ,
4ZR8 ,
5BT8 ,
5CC8 ,
5CM7 ,
5CY4 ,
5DD7 ,
5E3I ,
5EKS ,
5EO6 ,
5HA4 ,
5IDV ,
5J3B ,
5KHA ,
5U7S ,
5UDF ,
5URB ,
5VMB ,
5VMK ,
5VPU ,
6AMY ,
6AMZ ,
6BNG ,
6BQY ,
6BQZ ,
6C49 ,
6CAU ,
6D6K ,
6MFM ,
6MPR ,
6MRF
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKILVTNAL PYANGPIHMG HLLGYIQADI WVRAMRAMGH DVTYVCADDA HGTAIMLRAE ANGISPEEQI ANVQKEHIRD FDGFGVHFDH YDSTHSDANK ARSTDIYIKN REAGNIAVRP VTQLFDPEKG MFLSDRFIKG TCPKCKSEDQ YGDSCEVCGT TYNATELLNP RSTLSGATPV EKSSDHYFFK LPNFAEYLQK WTRDEGRLPL SIANKLDEWF EAGLADWDIS RDAPYFGFEI PDAPNKYFYV WVDAPIGYMS SFENYIKTKR PDLNFDDFWK KDSQNEVYHF IGKDIVYFHA LFWPAMLEGA NYRTPTGLFV NGFLTVNGQK MSKSRGTFIK AETYLQHLNP EYLRYYFASK LSDKVEDSDL NLDDFVQKVN SDLVGKVVNI ASRCAKFINS SFNNTLSSTC AESDLVQSFI DAGDSIAAAY EAREFSTAIR EIMALADRAN QYIDEKKPWA LAKQEGQEQQ VLDVCSVGIN LFRQLAVYLA PVLPTLAQQV QDFLKLESFD FESRKQILVS HEIAQFQPLM QRVDPKAVAA MVDASKESLG TPAPQATKAA KKEKSAEKKA APTPAVGEAE IIGIEDFLKV DLRVAQVVEA GTVEGSDKLL QLTLDVGEAE PRNVFSGIRS QYAPEDLKGK LVVMVANLAP RKMRFGISNG MVLAAGNGEG IFIISPDSGA KPGDKVS
NT Sequence
gtgcgtaaaa ttttagtcac caatgccctc ccttatgcca atggtccaat tcatatgggt catttactcg gttatatcca ggcagacatc tgggttcgtg ccatgcgggc aatgggtcat gacgtgactt atgtatgtgc ggatgatgct cacggtacag caattatgct acgtgccgaa gcaaatggta ttagcccaga agagcaaatc gcgaatgttc aaaaagaaca catccgtgat tttgacggtt ttggcgtaca tttcgatcat tatgattcaa ctcatagtga tgcaaacaaa gctcgttcaa cagatatcta tattaaaaac cgtgaagccg gaaatattgc agttcgacct gtaactcaat tatttgatcc ggaaaaaggt atgttcttgt ctgaccgttt cattaagggt acctgtccaa aatgtaagtc agaggatcaa tacggcgact catgtgaagt gtgtggtaca acttataatg cgactgagtt actcaacccg cgttcgactt taagtggtgc aacaccagtt gaaaaatcat cagatcacta tttcttcaag cttccaaact ttgctgaata tttgcaaaaa tggacccgcg atgaaggtcg cttacctctt tcaattgcta acaaattgga tgaatggttt gaagcaggtt tagcagactg ggatatttct cgtgatgctc cttattttgg ttttgaaatt ccggatgcgc caaataaata tttctatgtg tgggtagatg ccccaatcgg ttatatgtct agttttgaaa actacatcaa aactaagcgc ccagacttga actttgatga tttctggaag aaagatagtc aaaatgaggt ttaccacttt attggtaaag acattgttta cttccatgct ttattctggc ctgcgatgct tgaaggggcg aactaccgca ctccaacagg cttattcgta aatggtttct tgactgtaaa tggacaaaaa atgtctaagt ctcgcggtac tttcattaaa gccgagacgt acttacaaca tctaaaccca gagtatttac gctattactt tgcttctaaa ctttcagata aagttgaaga ctctgacttg aaccttgatg actttgtgca gaaagtaaat tcagatttag tcggtaaagt agttaatatt gctagtcgct gtgctaagtt cattaacagc agctttaaca atacgctatc aagtacatgt gctgaatctg acttagtaca aagctttatt gatgctggtg attcaattgc tgctgcatat gaagcgcgtg aattctctac ggcgatccgt gaaattatgg cgcttgctga ccgcgcaaac caatatattg atgagaaaaa accttgggct cttgctaaac aagaaggtca agaacaacaa gttcttgatg tatgctctgt cggtatcaac ttattccgtc aattagcagt gtacttagct ccagttctgc caactttagc tcagcaagtt caagatttct taaaacttga aagcttcgat ttcgagtcac gtaaacaaat tcttgtaagc catgagattg cacagttcca acctcttatg cagcgtgttg atccaaaagc tgttgcagcc atggttgatg cttctaaaga gtctttaggt actcctgcgc ctcaagcgac taaagcagcg aaaaaagaga aatctgccga gaaaaaagca gcccctactc cagcagttgg tgaagctgaa attattggta ttgaagactt cttaaaagtt gacttacgtg ttgctcaggt cgttgaggca ggtacagttg agggttcaga taaattactt caactgacgt tagacgtagg tgaagctgaa ccacgtaatg tatttagtgg tattcgtagt caatatgcgc cagaagacct aaaaggaaaa ttggtcgtta tggtggctaa ccttgcacca cgtaaaatgc gttttggtat ttcaaacggt atggtacttg ctgccggaaa tggtgaaggt attttcatta tttcaccaga tagcggtgca aaacctggcg ataaagtttc ttaa
Details for AcbaB.10201.a.A1.GE39848
HARVESTED ON:
1/26/2016
SEQUENCED ON:
2/12/2016
EXPECTED MW:
79kDa
OBSERVED MW:
79kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for AcbaB.10201.a.A2.GE39849
HARVESTED ON:
1/26/2016
SEQUENCED ON:
2/12/2016
EXPECTED MW:
64kDa
OBSERVED MW:
62kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for AcbaB.10201.a.A3.GE39850
HARVESTED ON:
1/26/2016
SEQUENCED ON:
2/12/2016
EXPECTED MW:
67kDa
OBSERVED MW:
67kDa
ANTIBIOTIC MARKER:
ampicillin
COUNT OF EXPRESSION COLONIES:
Many (50-100)
TOTAL EXPRESSION LEVEL:
High Expression
SOLUBLE EXPRESSION LEVEL
High Expression
EXPRESSION HOST:
data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for AcbaB.10201.a.A1.PW37926
PURIFICATION DATe:
7/11/2016
CONCENTRATION:
33.5mg/ml
OBSERVED MW:
78kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
11
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for AcbaB.10201.a.A2.PW37927
PURIFICATION DATe:
7/11/2016
CONCENTRATION:
23.5mg/ml
OBSERVED MW:
64kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
15
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable
Details for AcbaB.10201.a.A2.PW37928
PURIFICATION DATe:
7/11/2016
CONCENTRATION:
40mg/ml
OBSERVED MW:
64kDa
EXPRESSION LEVEL:
High Expression
PROTEIN PURIFICATION BUFFER:
25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:
data unavailable
VIAL COUNT (approx.):
3
VIAL VOLUME:
200µl
PERCENT IDENTITY:
data unavailable
PERCENT COVERAGE:
data unavailable