SSGCID: Target Page

AcbaB.01675.a

phospholipase, patatin family

CENTER ID:	AcbaB.01675.a
ORGANISM:	Acinetobacter baumannii ATCC 17978 / NCDC KC 755
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaB.01675.a.B2.GE41677	mature protein		35	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|470.1999.peg.792
GenBank:	ABO13204.2
PGAT:	acinetobacter:A1S_2792
UniProt:	V5VA50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKQLKLWVLG LSMVLAGCQT TSHLSALKPQ TAEAYSRFVD QPFAQLKKQH KKPVVALVLG SGGARGYAHI GVIEVLEQHG IRPDFIVGTS AGSIVGAIYA SGKTPDELRD TALKMKAADV RDISIGLKGF FDGKKVEDYV NQQVNNLPLE KMKIPMYVVA TELKHGTKTV FNYGNTGQAV RASASIPSMF VPTKIGKSEY VDGGLVSPVP VEVARDLGAD VIIAVDILAQ PIYTETSNVW GLFNQNINIM QGRLAAEELQ YADVVIQPDL REKAHIFDVK GREATMKAGI DAANAKLSDI QFAIDEKIAE QNLSTDGLSA QTQ

NT Sequence

atgaaacagc ttaaattgtg ggttctcgga ctttctatgg tgttggccgg gtgccaaacc acctcacact tatctgcctt aaaaccgcaa acggcagagg catactcacg tttcgtcgat cagccttttg cacagcttaa gaagcagcat aagaaaccag tagtcgcatt agtactaggt agtggtggtg cacgaggata tgcacatatt ggtgttattg aagttttaga acagcatggt attcgtcctg atttcattgt tggaaccagt gcaggaagta ttgtcggagc aatttatgcg agtggaaaaa cgcctgatga actgcgcgat actgcattga aaatgaaagc ggctgatgtt cgtgacatca gtattggttt aaaaggtttc tttgacggca aaaaagttga agactacgta aatcagcaag taaataactt gccgctcgaa aaaatgaaaa ttccgatgta tgtggtggca acagagctaa agcatggtac caaaaccgta tttaattatg gcaatacggg gcaagccgtg agagcatcag cctcaatacc aagcatgttt gtaccgacta aaattggtaa atctgaatat gtagatggcg gtttagtcag tccggtacct gttgaagtag cccgtgattt aggagccgat gtcattattg ctgtcgatat tctggctcaa cccatttata cggaaacctc aaatgtatgg ggactcttta accagaatat taatattatg caaggacgtt tggctgccga agaacttcaa tatgcagatg tagtaattca accggattta agagaaaaag cacatatttt tgatgtaaaa ggccgtgaag caaccatgaa ggctggtata gatgcagcaa atgcaaaact gtctgatatt cagtttgcga ttgatgaaaa aatagctgaa cagaatctat ctactgacgg tttaagtgcg caaacacaat aa

Details for AcbaB.01675.a.B2.GE41677

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHYS RFVDQPFAQL KKQHKKPVVA LVLGSGGARG YAHIGVIEVL EQHGIRPDFI VGTSAGSIVG AIYASGKTPD ELRDTALKMK AADVRDISIG LKGFFDGKKV EDYVNQQVNN LPLEKMKIPM YVVATELKHG TKTVFNYGNT GQAVRASASI PSMFVPTKIG KSEYVDGGLV SPVPVEVARD LGADVIIAVD ILAQPIYTET SNVWGLFNQN INIMQGRLAA EELQYADVVI QPDLREKAHI FDVKGREATM KAGIDAANAK LSDIQFAIDE KIAEQNLSTD GLSAQTQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattactcac gtttcgtcga tcagcctttt gcacagctta agaagcagca taagaaacca gtagtcgcat tagtactagg tagtggtggt gcacgaggat atgcacatat tggtgttatt gaagttttag aacagcatgg tattcgtcct gatttcattg ttggaaccag tgcaggaagt attgtcggag caatttatgc gagtggaaaa acgcctgatg aactgcgcga tactgcattg aaaatgaaag cggctgatgt tcgtgacatc agtattggtt taaaaggttt ctttgacggc aaaaaagttg aagactacgt aaatcagcaa gtaaataact tgccgctcga aaaaatgaaa attccgatgt atgtggtggc aacagagcta aagcatggta ccaaaaccgt atttaattat ggcaatacgg ggcaagccgt gagagcatca gcctcaatac caagcatgtt tgtaccgact aaaattggta aatctgaata tgtagatggc ggtttagtca gtccggtacc tgttgaagta gcccgtgatt taggagccga tgtcattatt gctgtcgata ttctggctca acccatttat acggaaacct caaatgtatg gggactcttt aaccagaata ttaatattat gcaaggacgt ttggctgccg aagaacttca atatgcagat gtagtaattc aaccggattt aagagaaaaa gcacatattt ttgatgtaaa aggccgtgaa gcaaccatga aggctggtat agatgcagca aatgcaaaac tgtctgatat tcagtttgcg attgatgaaa aaatagctga acagaatcta tctactgacg gtttaagtgc gcaaacacaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat