SSGCID: Target Page

AcbaB.01370.a

phosphomannomutase

CENTER ID:	AcbaB.01370.a
ORGANISM:	Acinetobacter baumannii ATCC 17978 / NCDC KC 755
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaB.01370.a.B1.GE41675	full length		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AcbaB.01370.a.B1.PW38388	full length		1	456

External Resources

RESOURCE	REFERENCE ID
GenBank:	ABO10561.2
PGAT:	acinetobacter:A1S_0066

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTLTCFKAY DIRGKLGTEL NEEIAYKIGR AYGQIYKPKT VVVGCDIRLS SEALKQATIR GLNDAGVNVL DLGMTGTEEV YFAAFHLDVQ GGIEVTASHN PMDYNGMKLV RENARPISAD TGLKEIQALA ETNNFEEVSQ KGTTQSYNIL PEFVDHLLTY IEPAKIRPLK LVVNAGNGAA GHVIDAIEEK FKALNVPVEF IKIHHEADGT FPNGIPNPIL IENRDSTRNA VLEHKADMGI AWDGDFDRCF LFDEKGQFIE GYYIVGLLAQ AFLIKQSGEK IVHDPRLVWN TFDIVDEYKG VAVQSKSGHA FIKDVMREHN AVYGGEMSAH HYFRDFAYCD SGMIPWLLTI ALLSETGQSL STLVENMIAK FPCSGEINFK VADTQTTIQK IFDFYADQNP QIDRTDGVSL DFGAWRFNVR ASNTEPLLRL NIESRADRQA QPMQYYVDEL TGLIQN

NT Sequence

atgactactt taacgtgttt taaagcttat gatatccgag gcaaactcgg caccgaattg aatgaagaaa tcgcctataa aattggccgc gcttacggac aaatctataa accaaaaact gtggttgtag gttgtgatat tcgtctaagc agtgaggctt taaaacaagc gaccatccgc ggtttaaatg atgcgggtgt gaatgtgctt gatttaggca tgacgggtac tgaagaagtg tactttgccg cttttcattt agacgtacaa ggtggtatag aagtaactgc gagtcataac ccaatggact ataacggtat gaaattggtt cgagaaaatg ctcgtccaat tagtgccgat actggtttaa aagaaattca ggctttagca gagaccaata attttgaaga agttagccaa aaaggtacaa cgcaaagcta taacattttg cctgagttcg ttgaccatct actgacttat attgaacctg caaaaatccg tccattaaag ttagtggtaa acgcgggtaa tggtgcagct ggtcatgtga ttgatgccat tgaagaaaaa tttaaagcgc ttaatgtacc ggttgaattt attaaaattc atcatgaagc agatggtact ttcccaaatg gcatccctaa cccaatttta attgaaaacc gtgacagtac ccgaaatgct gttcttgagc ataaagcaga tatgggtatt gcatgggacg gcgactttga ccgctgtttc ttgttcgatg aaaaaggaca attcattgag ggctactata ttgttggcct attggctcaa gctttcttaa tcaaacagtc aggcgaaaaa attgtgcatg acccacgttt agtgtggaac acatttgata tcgtcgacga atataaaggt gttgctgtac agtcaaaatc ggggcatgca tttattaaag atgttatgcg tgaacacaat gccgtatacg gcggtgaaat gagtgcccac cactatttcc gtgattttgc ttactgtgac agtggtatga ttccttggtt actcaccatt gctctactct ctgaaacagg acaatctctc tcaacactcg ttgaaaatat gattgccaag ttcccttgta gtggtgaaat caactttaaa gtagccgata cgcaaactac gattcaaaag attttcgact tctatgccga tcaaaatcca caaattgacc gtaccgatgg tgtaagcctc gactttggcg catggcgctt taacgtccgt gcttcaaata ctgagccttt gttacgtctt aatattgaaa gccgtgcaga tcgtcaagct cagccaatgc aatattatgt agatgagttg actggattaa ttcagaacta a

Details for AcbaB.01370.a.B1.GE41675

HARVESTED ON:	6/19/2017
SEQUENCED ON:	6/22/2017
EXPECTED MW:	52kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT TLTCFKAYDI RGKLGTELNE EIAYKIGRAY GQIYKPKTVV VGCDIRLSSE ALKQATIRGL NDAGVNVLDL GMTGTEEVYF AAFHLDVQGG IEVTASHNPM DYNGMKLVRE NARPISADTG LKEIQALAET NNFEEVSQKG TTQSYNILPE FVDHLLTYIE PAKIRPLKLV VNAGNGAAGH VIDAIEEKFK ALNVPVEFIK IHHEADGTFP NGIPNPILIE NRDSTRNAVL EHKADMGIAW DGDFDRCFLF DEKGQFIEGY YIVGLLAQAF LIKQSGEKIV HDPRLVWNTF DIVDEYKGVA VQSKSGHAFI KDVMREHNAV YGGEMSAHHY FRDFAYCDSG MIPWLLTIAL LSETGQSLST LVENMIAKFP CSGEINFKVA DTQTTIQKIF DFYADQNPQI DRTDGVSLDF GAWRFNVRAS NTEPLLRLNI ESRADRQAQP MQYYVDELTG LIQN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacta ctttaacgtg ttttaaagct tatgatatcc gaggcaaact cggcaccgaa ttgaatgaag aaatcgccta taaaattggc cgcgcttacg gacaaatcta taaaccaaaa actgtggttg taggttgtga tattcgtcta agcagtgagg ctttaaaaca agcgaccatc cgcggtttaa atgatgcggg tgtgaatgtg cttgatttag gcatgacggg tactgaagaa gtgtactttg ccgcttttca tttagacgta caaggtggta tagaagtaac tgcgagtcat aacccaatgg actataacgg tatgaaattg gttcgagaaa atgctcgtcc aattagtgcc gatactggtt taaaagaaat tcaggcttta gcagagacca ataattttga agaagttagc caaaaaggta caacgcaaag ctataacatt ttgcctgagt tcgttgacca tctactgact tatattgaac ctgcaaaaat ccgtccatta aagttagtgg taaacgcggg taatggtgca gctggtcatg tgattgatgc cattgaagaa aaatttaaag cgcttaatgt accggttgaa tttattaaaa ttcatcatga agcagatggt actttcccaa atggcatccc taacccaatt ttaattgaaa accgtgacag tacccgaaat gctgttcttg agcataaagc agatatgggt attgcatggg acggcgactt tgaccgctgt ttcttgttcg atgaaaaagg acaattcatt gagggctact atattgttgg cctattggct caagctttct taatcaaaca gtcaggcgaa aaaattgtgc atgacccacg tttagtgtgg aacacatttg atatcgtcga cgaatataaa ggtgttgctg tacagtcaaa atcggggcat gcatttatta aagatgttat gcgtgaacac aatgccgtat acggcggtga aatgagtgcc caccactatt tccgtgattt tgcttactgt gacagtggta tgattccttg gttactcacc attgctctac tctctgaaac aggacaatct ctctcaacac tcgttgaaaa tatgattgcc aagttccctt gtagtggtga aatcaacttt aaagtagccg atacgcaaac tacgattcaa aagattttcg acttctatgc cgatcaaaat ccacaaattg accgtaccga tggtgtaagc ctcgactttg gcgcatggcg ctttaacgtc cgtgcttcaa atactgagcc tttgttacgt cttaatattg aaagccgtgc agatcgtcaa gctcagccaa tgcaatatta tgtagatgag ttgactggat taattcagaa ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for AcbaB.01370.a.B1.PW38388

PURIFICATION DATe:	11/7/2017
CONCENTRATION:	42.22mg/ml
OBSERVED MW:	49kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	11
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

IMAC->SEC

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACTA CTTTAACGTG TTTTAAAGCT TATGATATCC GAGGCAAACT CGGCACCGAA TTGAATGAAG AAATCGCCTA TAAAATTGGC CGCGCTTACG GACAAATCTA TAAACCAAAA ACTGTGGTTG TAGGTTGTGA TATTCGTCTA AGCAGTGAGG CTTTAAAACA AGCGACCATC CGCGGTTTAA ATGATGCGGG TGTGAATGTG CTTGATTTAG GCATGACGGG TACTGAAGAA GTGTACTTTG CCGCTTTTCA TTTAGACGTA CAAGGTGGTA TAGAAGTAAC TGCGAGTCAT AACCCAATGG ACTATAACGG TATGAAATTG GTTCGAGAAA ATGCTCGTCC AATTAGTGCC GATACTGGTT TAAAAGAAAT TCAGGCTTTA GCAGAGACCA ATAATTTTGA AGAAGTTAGC CAAAAAGGTA CAACGCAAAG CTATAACATT TTGCCTGAGT TCGTTGACCA TCTACTGACT TATATTGAAC CTGCAAAAAT CCGTCCATTA AAGTTAGTGG TAAACGCGGG TAATGGTGCA GCTGGTCATG TGATTGATGC CATTGAAGAA AAATTTAAAG CGCTTAATGT ACCGGTTGAA TTTATTAAAA TTCATCATGA AGCAGATGGT ACTTTCCCAA ATGGCATCCC TAACCCAATT TTAATTGAAA ACCGTGACAG TACCCGAAAT GCTGTTCTTG AGCATAAAGC AGATATGGGT ATTGCATGGG ACGGCGACTT TGACCGCTGT TTCTTGTTCG ATGAAAAAGG ACAATTCATT GAGGGCTACT ATATTGTTGG CCTATTGGCT CAAGCTTTCT TAATCAAACA GTCAGGCGAA AAAATTGTGC ATGACCCACG TTTAGTGTGG AACACATTTG ATATCGTCGA CGAATATAAA GGTGTTGCTG TACAGTCAAA ATCGGGGCAT GCATTTATTA AAGATGTTAT GCGTGAACAC AATGCCGTAT ACGGCGGTGA AATGAGTGCC CACCACTATT TCCGTGATTT TGCTTACTGT GACAGTGGTA TGATTCCTTG GTTACTCACC ATTGCTCTAC TCTCTGAAAC AGGACAATCT CTCTCAACAC TCGTTGAAAA TATGATTGCC AAGTTCCCTT GTAGTGGTGA AATCAACTTT AAAGTAGCCG ATACGCAAAC TACGATTCAA AAGATTTTCG ACTTCTATGC CGATCAAAAT CCACAAATTG ACCGTACCGA TGGTGTAAGC CTCGACTTTG GCGCATGGCG CTTTAACGTC CGTGCTTCAA ATACTGAGCC TTTGTTACGT CTTAATATTG AAAGCCGTGC AGATCGTCAA GCTCAGCCAA TGCAATATTA TGTAGATGAG TTGACTGGAT TAATTCAGAA CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT