AcbaA.00166.a

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID: AcbaA.00166.a
ORGANISM: Acinetobacter baumannii ACICU
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaA.00166.a.DG11.GE00585 full length 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AcbaA.00166.a.DG11.PD00438 full length 1 301
AcbaA.00166.a.DG11.PD00466 full length 1 301

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|405416.6.peg.3548
RefSeq: YP_001848184.1
UniProt: B2I1I9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKQRTLNRV VKASGIGLHS GQKVMINFIP HTVDGGIVFR RIDLDPPVDI PANALLIQEA FMCSNLVTGD IKVGTIEHVM SAIAGLGIDN LIVEVSASEV PIMDGSAGPF IYLLMQGGLR EQDAPKKFIK ILKPVEALID DKKAIFSPHN GFQLNFTIDF DHPAFAKEYQ SATIDFSTET FVYEVSEART FGFMKDLDYL KANNLALGAS LDNAIGVDDT GVVNEEGLRF ADEFVRHKIL DAVGDLYLLG HQIIAKFDGY KSGHALNNQL LRNVQSDPSN YEIVTFDDEK DCPIPYVSVT
NT Sequence
atggtgaaac agcgtactct caatcgtgtg gtaaaagcga gtggaatagg tcttcatagc ggtcaaaaag tgatgatcaa tttcattcca cataccgtgg atggaggtat tgtatttcgc cgtatcgatt tggatccacc tgtcgatatt cctgctaatg cattgctgat tcaagaagca tttatgtgtt caaatcttgt cactggcgat attaaagtcg ggacaatcga acatgtgatg agtgcgattg ccggtttagg aatcgataac ttaattgtgg aagtgtccgc atccgaagtt ccaattatgg atggtagtgc tggtccattt atttatttgc tcatgcaagg tggcttgcgt gaacaagatg ctcctaagaa atttataaaa atattaaagc cagttgaggc tttaattgat gataaaaaag caatattcag cccgcataat ggctttcagc ttaactttac gattgatttt gatcatcctg catttgccaa agagtatcag tctgcaacta tcgatttttc tactgaaacg tttgtgtatg aagtcagtga ggcacgaact tttggtttta tgaaagactt ggattatctc aaagcaaata atttagcttt aggagcaagt ctagataatg caattggtgt agatgatacg ggtgttgtga acgaagaagg attacgattt gccgatgagt ttgttcgtca taaaatttta gatgcagttg gtgatttgta tttacttggt catcaaatta ttgccaagtt tgatggctat aaatcaggac atgccttaaa taatcagcta ttacgcaatg ttcaaagcga tccgagtaat tatgaaattg taacatttga tgacgagaaa gactgtccaa ttccatacgt gagtgtgaca taa
Details for AcbaA.00166.a.DG11.GE00585
HARVESTED ON: 3/11/2016
SEQUENCED ON: 10/25/2017
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG SMVKQRTLNR VVKASGIGLH SGQKVMINFI PHTVDGGIVF RRIDLDPPVD IPANALLIQE AFMCSNLVTG DIKVGTIEHV MSAIAGLGID NLIVEVSASE VPIMDGSAGP FIYLLMQGGL CEQDAPKKFI KILKPVEALI DDKKAIFSPH NGFQLNFTID FDHPAFAKEY QSATIDFSTE TFVYEVSEAR TFGFMKDLDY LKANNLALGA SLDNAIGVDD TGVVNEEGLR FADEFVRHKI LDAVGDLYLL GHQIIAKFDG YKSGHALNNQ LLRNVQSDPS NYEIVTFND
Validated NT Sequence
ctcgtcgttg aaagtaacga tctcgtagtt agacggatct gattgcacgt tgcgcaggag ctggttgttc agggcatgac cagatttata gccatcgaac ttagcgataa tctggtggcc cagcaaatac agatcgccta ctgcgtccag aattttgtga cgcacgaact cgtcagcgaa acgcaggccc tcctcgttta caacgccggt atcgtccaca ccaatagcat tatccagaga tgcgcccagt gccaggttgt tagccttcag ataatccagg tctttcataa agccgaaggt gcgggcttca gaaacctcat aaacgaaggt ttcagtagag aaatcgatgg tggcagactg gtattctttg gcaaaagccg ggtgatcaaa atcgatagta aagttcagtt ggaaaccgtt atgcggggag aagatggctt tcttgtcgtc tatcagggct tctaccggct tcagaatctt aatgaatttc ttcggtgcgt cttgttcaca gagaccaccc tgcattaaca gatagatgaa aggaccagca gaaccgtcca taataggaac ttcggaagcg gaaacttcaa ctatgaggtt gtcgatgccc agacccgcga tagcagacat aacgtgttcg atagtgccaa ccttaatatc accggtaacg aggttagagc acataaatgc ttcttgaatc aacagggcgt tggccgggat gtcaaccggc gggtcgaggt cgatacgacg gaaaacgata ccaccatcaa cagtatgtgg gataaagttg atcatcactt tctgaccgga gtgcaggccg atgccggacg ctttaacgac gcgattcagg gtacgctgtt tgaccatgga tccaccgatt tgttcacggt gtgcttcgat aatgtcgtta tcttccatgt ccagatcttc cggagtctgg tctgcctgaa tacggatacc gtcgtacaga aagcgcaggg aatccatttc cttgccctgg cgtttagcga atgcttccat caggcgacgc agcggggtgg tcttcttaat cttgaagaag atttcgctgc taccgtcgct aaccttcagg ttgatgtggg tctccggctt cacttccgnn nttacttcgc cgctatggtg gtggtggtgg tggga
Expected Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMVK QRTLNRVVKA SGIGLHSGQK VMINFIPHTV DGGIVFRRID LDPPVDIPAN ALLIQEAFMC SNLVTGDIKV GTIEHVMSAI AGLGIDNLIV EVSASEVPIM DGSAGPFIYL LMQGGLCEQD APKKFIKILK PVEALIDDKK AIFSPHNGFQ LNFTIDFDHP AFAKEYQSAT IDFSTETFVY EVSEARTFGF MKDLDYLKAN NLALGASLDN AIGVDDTGVV NEEGLRFADE FVRHKILDAV GDLYLLGHQI IAKFDGYKSG HALNNQLLRN VQSDPSNYEI VTFNDEKDCP IPYVSVT
Full NT Sequence (Expression Vector + Insert)
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg ctcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca tgtcccacca ccaccaccac catagcggcg aagtaaaacc ggaagtgaag ccggagaccc acatcaacct gaaggttagc gacggtagca gcgaaatctt cttcaagatt aagaagacca ccccgctgcg tcgcctgatg gaagcattcg ctaaacgcca gggcaaggaa atggattccc tgcgctttct gtacgacggt atccgtattc aggcagacca gactccggaa gatctggaca tggaagataa cgacattatc gaagcacacc gtgaacaaat cggtggatcc atggtcaaac agcgtaccct gaatcgcgtc gttaaagcgt ccggcatcgg cctgcactcc ggtcagaaag tgatgatcaa ctttatccca catactgttg atggtggtat cgttttccgt cgtatcgacc tcgacccgcc ggttgacatc ccggccaacg ccctgttgat tcaagaagca tttatgtgct ctaacctcgt taccggtgat attaaggttg gcactatcga acacgttatg tctgctatcg cgggtctggg catcgacaac ctcatagttg aagtttccgc ttccgaagtt cctattatgg acggttctgc tggtcctttc atctatctgt taatgcaggg tggtctctgt gaacaagacg caccgaagaa attcattaag attctgaagc cggtagaagc cctgatagac gacaagaaag ccatcttctc cccgcataac ggtttccaac tgaactttac tatcgatttt gatcacccgg cttttgccaa agaataccag tctgccacca tcgatttctc tactgaaacc ttcgtttatg aggtttctga agcccgcacc ttcggcttta tgaaagacct ggattatctg aaggctaaca acctggcact gggcgcatct ctggataatg ctattggtgt ggacgatacc ggcgttgtaa acgaggaggg cctgcgtttc gctgacgagt tcgtgcgtca caaaattctg gacgcagtag gcgatctgta tttgctgggc caccagatta tcgctaagtt cgatggctat aaatctggtc atgccctgaa caaccagctc ctgcgcaacg tgcaatcaga tccgtctaac tacgagatcg ttactttcaa cgacgagaag gactgtccga tcccatacgt tagcgtcacc tgataagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaacttga acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc
Details for AcbaA.00166.a.DG11.PD00438
PURIFICATION DATe: 3/21/2016
CONCENTRATION: 4.7mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM HEPES pH 7, 150mM NaCl, 2 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG SMVKQRTLNR VVKASGIGLH SGQKVMINFI PHTVDGGIVF RRIDLDPPVD IPANALLIQE AFMCSNLVTG DIKVGTIEHV MSAIAGLGID NLIVEVSASE VPIMDGSAGP FIYLLMQGGL CEQDAPKKFI KILKPVEALI DDKKAIFSPH NGFQLNFTID FDHPAFAKEY QSATIDFSTE TFVYEVSEAR TFGFMKDLDY LKANNLALGA SLDNAIGVDD TGVVNEEGLR FADEFVRHKI LDAVGDLYLL GHQIIAKFDG YKSGHALNNQ LLRNVQSDPS NYEIVTFND
Validated NT Sequence
ctcgtcgttg aaagtaacga tctcgtagtt agacggatct gattgcacgt tgcgcaggag ctggttgttc agggcatgac cagatttata gccatcgaac ttagcgataa tctggtggcc cagcaaatac agatcgccta ctgcgtccag aattttgtga cgcacgaact cgtcagcgaa acgcaggccc tcctcgttta caacgccggt atcgtccaca ccaatagcat tatccagaga tgcgcccagt gccaggttgt tagccttcag ataatccagg tctttcataa agccgaaggt gcgggcttca gaaacctcat aaacgaaggt ttcagtagag aaatcgatgg tggcagactg gtattctttg gcaaaagccg ggtgatcaaa atcgatagta aagttcagtt ggaaaccgtt atgcggggag aagatggctt tcttgtcgtc tatcagggct tctaccggct tcagaatctt aatgaatttc ttcggtgcgt cttgttcaca gagaccaccc tgcattaaca gatagatgaa aggaccagca gaaccgtcca taataggaac ttcggaagcg gaaacttcaa ctatgaggtt gtcgatgccc agacccgcga tagcagacat aacgtgttcg atagtgccaa ccttaatatc accggtaacg aggttagagc acataaatgc ttcttgaatc aacagggcgt tggccgggat gtcaaccggc gggtcgaggt cgatacgacg gaaaacgata ccaccatcaa cagtatgtgg gataaagttg atcatcactt tctgaccgga gtgcaggccg atgccggacg ctttaacgac gcgattcagg gtacgctgtt tgaccatgga tccaccgatt tgttcacggt gtgcttcgat aatgtcgtta tcttccatgt ccagatcttc cggagtctgg tctgcctgaa tacggatacc gtcgtacaga aagcgcaggg aatccatttc cttgccctgg cgtttagcga atgcttccat caggcgacgc agcggggtgg tcttcttaat cttgaagaag atttcgctgc taccgtcgct aaccttcagg ttgatgtggg tctccggctt cacttccgnn nttacttcgc cgctatggtg gtggtggtgg tggga
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMVK QRTLNRVVKA SGIGLHSGQK VMINFIPHTV DGGIVFRRID LDPPVDIPAN ALLIQEAFMC SNLVTGDIKV GTIEHVMSAI AGLGIDNLIV EVSASEVPIM DGSAGPFIYL LMQGGLCEQD APKKFIKILK PVEALIDDKK AIFSPHNGFQ LNFTIDFDHP AFAKEYQSAT IDFSTETFVY EVSEARTFGF MKDLDYLKAN NLALGASLDN AIGVDDTGVV NEEGLRFADE FVRHKILDAV GDLYLLGHQI IAKFDGYKSG HALNNQLLRN VQSDPSNYEI VTFNDEKDCP IPYVSVT
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGGTCAAAC AGCGTACCCT GAATCGCGTC GTTAAAGCGT CCGGCATCGG CCTGCACTCC GGTCAGAAAG TGATGATCAA CTTTATCCCA CATACTGTTG ATGGTGGTAT CGTTTTCCGT CGTATCGACC TCGACCCGCC GGTTGACATC CCGGCCAACG CCCTGTTGAT TCAAGAAGCA TTTATGTGCT CTAACCTCGT TACCGGTGAT ATTAAGGTTG GCACTATCGA ACACGTTATG TCTGCTATCG CGGGTCTGGG CATCGACAAC CTCATAGTTG AAGTTTCCGC TTCCGAAGTT CCTATTATGG ACGGTTCTGC TGGTCCTTTC ATCTATCTGT TAATGCAGGG TGGTCTCTGT GAACAAGACG CACCGAAGAA ATTCATTAAG ATTCTGAAGC CGGTAGAAGC CCTGATAGAC GACAAGAAAG CCATCTTCTC CCCGCATAAC GGTTTCCAAC TGAACTTTAC TATCGATTTT GATCACCCGG CTTTTGCCAA AGAATACCAG TCTGCCACCA TCGATTTCTC TACTGAAACC TTCGTTTATG AGGTTTCTGA AGCCCGCACC TTCGGCTTTA TGAAAGACCT GGATTATCTG AAGGCTAACA ACCTGGCACT GGGCGCATCT CTGGATAATG CTATTGGTGT GGACGATACC GGCGTTGTAA ACGAGGAGGG CCTGCGTTTC GCTGACGAGT TCGTGCGTCA CAAAATTCTG GACGCAGTAG GCGATCTGTA TTTGCTGGGC CACCAGATTA TCGCTAAGTT CGATGGCTAT AAATCTGGTC ATGCCCTGAA CAACCAGCTC CTGCGCAACG TGCAATCAGA TCCGTCTAAC TACGAGATCG TTACTTTCAA CGACGAGAAG GACTGTCCGA TCCCATACGT TAGCGTCACC TGATAAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTTGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TAGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CTGCTCATGT TTGACAGCTT ATC
Details for AcbaA.00166.a.DG11.PD00466
PURIFICATION DATe: 10/14/2016
CONCENTRATION: 5.95mg/ml
OBSERVED MW: 44kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25mM HEPES pH 7.5, 150mM NaCl, 2mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 85
Protocol Notes
notes unavailable
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG SMVKQRTLNR VVKASGIGLH SGQKVMINFI PHTVDGGIVF RRIDLDPPVD IPANALLIQE AFMCSNLVTG DIKVGTIEHV MSAIAGLGID NLIVEVSASE VPIMDGSAGP FIYLLMQGGL CEQDAPKKFI KILKPVEALI DDKKAIFSPH NGFQLNFTID FDHPAFAKEY QSATIDFSTE TFVYEVSEAR TFGFMKDLDY LKANNLALGA SLDNAIGVDD TGVVNEEGLR FADEFVRHKI LDAVGDLYLL GHQIIAKFDG YKSGHALNNQ LLRNVQSDPS NYEIVTFND
Validated NT Sequence
ctcgtcgttg aaagtaacga tctcgtagtt agacggatct gattgcacgt tgcgcaggag ctggttgttc agggcatgac cagatttata gccatcgaac ttagcgataa tctggtggcc cagcaaatac agatcgccta ctgcgtccag aattttgtga cgcacgaact cgtcagcgaa acgcaggccc tcctcgttta caacgccggt atcgtccaca ccaatagcat tatccagaga tgcgcccagt gccaggttgt tagccttcag ataatccagg tctttcataa agccgaaggt gcgggcttca gaaacctcat aaacgaaggt ttcagtagag aaatcgatgg tggcagactg gtattctttg gcaaaagccg ggtgatcaaa atcgatagta aagttcagtt ggaaaccgtt atgcggggag aagatggctt tcttgtcgtc tatcagggct tctaccggct tcagaatctt aatgaatttc ttcggtgcgt cttgttcaca gagaccaccc tgcattaaca gatagatgaa aggaccagca gaaccgtcca taataggaac ttcggaagcg gaaacttcaa ctatgaggtt gtcgatgccc agacccgcga tagcagacat aacgtgttcg atagtgccaa ccttaatatc accggtaacg aggttagagc acataaatgc ttcttgaatc aacagggcgt tggccgggat gtcaaccggc gggtcgaggt cgatacgacg gaaaacgata ccaccatcaa cagtatgtgg gataaagttg atcatcactt tctgaccgga gtgcaggccg atgccggacg ctttaacgac gcgattcagg gtacgctgtt tgaccatgga tccaccgatt tgttcacggt gtgcttcgat aatgtcgtta tcttccatgt ccagatcttc cggagtctgg tctgcctgaa tacggatacc gtcgtacaga aagcgcaggg aatccatttc cttgccctgg cgtttagcga atgcttccat caggcgacgc agcggggtgg tcttcttaat cttgaagaag atttcgctgc taccgtcgct aaccttcagg ttgatgtggg tctccggctt cacttccgnn nttacttcgc cgctatggtg gtggtggtgg tggga
Expressed Protein Sequence
MSHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMVK QRTLNRVVKA SGIGLHSGQK VMINFIPHTV DGGIVFRRID LDPPVDIPAN ALLIQEAFMC SNLVTGDIKV GTIEHVMSAI AGLGIDNLIV EVSASEVPIM DGSAGPFIYL LMQGGLCEQD APKKFIKILK PVEALIDDKK AIFSPHNGFQ LNFTIDFDHP AFAKEYQSAT IDFSTETFVY EVSEARTFGF MKDLDYLKAN NLALGASLDN AIGVDDTGVV NEEGLRFADE FVRHKILDAV GDLYLLGHQI IAKFDGYKSG HALNNQLLRN VQSDPSNYEI VTFNDEKDCP IPYVSVT
Full NT Sequence (Expression Vector + Insert)
ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG CTCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA TGTCCCACCA CCACCACCAC CATAGCGGCG AAGTAAAACC GGAAGTGAAG CCGGAGACCC ACATCAACCT GAAGGTTAGC GACGGTAGCA GCGAAATCTT CTTCAAGATT AAGAAGACCA CCCCGCTGCG TCGCCTGATG GAAGCATTCG CTAAACGCCA GGGCAAGGAA ATGGATTCCC TGCGCTTTCT GTACGACGGT ATCCGTATTC AGGCAGACCA GACTCCGGAA GATCTGGACA TGGAAGATAA CGACATTATC GAAGCACACC GTGAACAAAT CGGTGGATCC ATGGTCAAAC AGCGTACCCT GAATCGCGTC GTTAAAGCGT CCGGCATCGG CCTGCACTCC GGTCAGAAAG TGATGATCAA CTTTATCCCA CATACTGTTG ATGGTGGTAT CGTTTTCCGT CGTATCGACC TCGACCCGCC GGTTGACATC CCGGCCAACG CCCTGTTGAT TCAAGAAGCA TTTATGTGCT CTAACCTCGT TACCGGTGAT ATTAAGGTTG GCACTATCGA ACACGTTATG TCTGCTATCG CGGGTCTGGG CATCGACAAC CTCATAGTTG AAGTTTCCGC TTCCGAAGTT CCTATTATGG ACGGTTCTGC TGGTCCTTTC ATCTATCTGT TAATGCAGGG TGGTCTCTGT GAACAAGACG CACCGAAGAA ATTCATTAAG ATTCTGAAGC CGGTAGAAGC CCTGATAGAC GACAAGAAAG CCATCTTCTC CCCGCATAAC GGTTTCCAAC TGAACTTTAC TATCGATTTT GATCACCCGG CTTTTGCCAA AGAATACCAG TCTGCCACCA TCGATTTCTC TACTGAAACC TTCGTTTATG AGGTTTCTGA AGCCCGCACC TTCGGCTTTA TGAAAGACCT GGATTATCTG AAGGCTAACA ACCTGGCACT GGGCGCATCT CTGGATAATG CTATTGGTGT GGACGATACC GGCGTTGTAA ACGAGGAGGG CCTGCGTTTC GCTGACGAGT TCGTGCGTCA CAAAATTCTG GACGCAGTAG GCGATCTGTA TTTGCTGGGC CACCAGATTA TCGCTAAGTT CGATGGCTAT AAATCTGGTC ATGCCCTGAA CAACCAGCTC CTGCGCAACG TGCAATCAGA TCCGTCTAAC TACGAGATCG TTACTTTCAA CGACGAGAAG GACTGTCCGA TCCCATACGT TAGCGTCACC TGATAAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTTGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTTGA ACAACACTCA ACCCTATCTC GGGCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TAGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CTGCTCATGT TTGACAGCTT ATC